A5073197925- Proteins in Food Systems
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Chemical Synthesis and Analysis
- Regulation of Appetite and Obesity
- Liquid Crystal Research Advancements
- Surfactants and Colloidal Systems
- Biochemical Analysis and Sensing Techniques
- Bacteriophages and microbial interactions
- Polysaccharides Composition and Applications
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Protein Structure and Dynamics
- Polysaccharides and Plant Cell Walls
- Food composition and properties
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Fluorine in Organic Chemistry
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Phytochemicals and Antioxidant Activities
- Digestive system and related health
- Protein Hydrolysis and Bioactive Peptides
- Adipose Tissue and Metabolism
Massey University
2015-2024
Portland State University
2023
Maurice Wilkins Centre
2020
University of Virginia
2020
Palmerston North Hospital
2015-2019
Eli Lilly (United States)
2001-2010
Plant & Food Research
2009
Indiana University – Purdue University Indianapolis
1992-2005
Indiana University School of Medicine
1992-2005
University of Auckland
2003
The plant growth-repressing DELLA proteins (DELLAs) are known to represent a convergence point in integration of multiple developmental and environmental signals planta, one which is hormone gibberellic acid (GA). Binding the liganded GA receptor (GID1/GA) N-terminal domain DELLAs required for GA-induced degradation via ubiquitin-proteasome pathway, thus derepressing growth. However, conformational changes upon binding GID1/GA, key understanding precise mechanism GID1/GA-mediated DELLAs,...
Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-linked N-acetylglucosamine moieties, attached via side chain linkages to serine oxygen, cysteine sulfur. The latter linkage is novel has helped establish new type of...
Yeast cells of Candida albicans are induced by serum at 37 °C to produce germ tubes, the first step in a transition from yeast hyphal growth. Previously, it has been shown that active component is not albumin but present dialysable fraction serum. In this study, induction germ-tube formation occur even presence added exogenous nitrogen sources and therefore signalled derepression. The was purified ion-exchange, reverse-phase size-exclusion chromatography identified NMR be d -glucose. Enzymic...
The anthocyanins responsible for the red color of kiwifruit were extracted in acidified ethanol and isolated by solid phase extraction (SPE) followed preparative HPLC. Five obtained subsequently identified as delphinidin 3-[2-(xylosyl)galactoside], 3-galactoside, cyanidin 3-glucoside a combination LC-MS/MS, GC-MS, 2D NMR. Delphinidin 3-[2-(xylosyl)galactoside] 3-galactoside have not previously been reported genus Actinidia.
The synthesis and characterization of the fluxional, d(10) cyclotriphosphazene complexes, [MLCl(2)] (M = Zn, Cd, Hg; L spiro-[(1,1'-biphenyl)-2,2'-dioxy]tetrakis(4-methyl-2-pyridyloxy)cyclotriphosphazene), are described. Single-crystal X-ray structures show that zinc complex has crystallized into two crystal forms: one as a tetrahedral species, with N(2)Cl(2) donor set in which geminal pair pendant pyridyloxy nitrogen atoms binds to zinc, other trigonal-bipyramidal (tbp) one, an N(3)Cl(2)...
To investigate the assimilation and production of juice metabolites by Saccharomyces cerevisiae during winemaking, we compared metabolite profiles 63 Sauvignon blanc (SB) grape juices collected over five harvesting seasons from different locations New Zealand before after fermentation commercial wine yeast strain EC1118 at 15 °C. Metabolite were obtained using gas chromatography-mass spectrometry nuclear magnetic resonance oenological parameters determined Fourier transform infrared...
NMR was used to study the thermodynamics of cis → trans isomerization for prolyl amide bonds in compounds shown. The magnitude Kt/c C-terminal esters is greater than corresponding amides, signifying stronger backbone stereoelectronic effects esters. Increasing steric bulk N-terminal residue from Ac- Ac-Gly- favors conformation. Incorporation a Phe Pro, however, shifts equilibrium favor conformation, via stabilizing aromatic−proline interaction.
A series of eight dipeptides the general formula Ac-Phe-Pro*-NHMe was synthesized and thermodynamics cis → trans isomerization about central amide bond were studied by NMR. Pro* represents following prolines: l-proline (Pro), l-trans-4-hydroxyproline (Hyp), l-cis-4-hydroxyproline (hyp), l-cis-4-methoxyproline (hyp[OMe]), l-trans-3-hydroxyproline (3-Hyp), l-cis-3-hydroxyproline (3-hyp), l-2,3-trans-3,4-cis-3,4-dihydroxyproline (DHP), l-2,3-cis-3,4-trans-3,4-dihydroxyproline (dhp). The...
Abstract The first total synthesis of glycocin F, a uniquely diglycosylated antimicrobial peptide bearing rare S‐linked N ‐acetylglucosamine (GlcNAc) moiety in addition to an O‐linked GlcNAc, has been accomplished using native chemical ligation strategy. synthetic and naturally occurring peptides were compared by HPLC, mass spectrometry, NMR CD spectroscopy, their stability towards chymotrypsin digestion activity measured. This is the comprehensive structural functional comparison with...
A shear-thickening polysaccharide from the New Zealand Black tree fern (Cyathea medullaris, commonly known as mamaku) extracted different age fronds (stage 1: young, stage 2: fully grown and 3: old) was characterised in terms of structure rheological properties. Constituent sugar analysis
Abstract We present here the high-resolution structure of an antiparallel DNA triplex in which a monomer para-twisted intercalating nucleic acid (para-TINA: (R)-1-O-[4-(1-pyrenylethynyl)phenylmethyl]glycerol) is covalently inserted as bulge third strand triplex. TINA potent modulator hybridization properties sequences with extremely useful when conjugated G-rich oligonucleotides. The insertion para-TINA between two guanines imparts high thermal stabilization (ΔTM = 9ºC) to and enhances...
The major anthocyanins of boysenberry fruit, a cross between Rubus loganbaccus and baileyanus Britt., were isolated by preparative high-performance liquid chromatography (HPLC). structures cyanidin-3-[2-(glucosyl)glucoside] (1) cyanidin-3-[2-(glucosyl)-6-(rhamnosyl)glucoside] (2) determined NMR in 1% DCOOD/D(2)O. An unusually high chemical shift (delta 2.5) is reported for H-5' '' cyanidin-3-[2-(glucosyl)glucoside].
Abstract An acetone:water (7:3) extract obtained from the leaves of Rumex obtusifolius was fractionated into procyanidin oligomer and polymer fractions using a linear gradient simple step method on Sephadex LH‐20. The chemical characteristics were studied by 13 C‐NMR spectroscopy, acid‐catalysed degradation in presence benzyl mercaptan, matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) MS electrospray ionisation (ESI) MS. showed that fraction consisted predominantly...
Prostate apoptosis response factor-4 (Par-4) is an ubiquitously expressed pro-apoptotic and tumour suppressive protein that can both activate cell-death mechanisms inhibit pro-survival factors. Par-4 contains a highly conserved coiled-coil region serves as the primary recognition domain for large number of binding partners. also tightly regulated by aforementioned partners post-translational modifications. Biophysical data obtained in present study indicate primarily comprises intrinsically...
Optically pure L‐(+)‐C α ‐methyl‐arginine and D‐(‐)‐C were synthesized. Experimental results indicated that DL‐ C methyl ester could be resolved by trypsin, but workup posed a technical difficulty. Chemical resolution at the stage of DL‐C ‐methyl‐ornithine, followed selective guanid‐ination using N,N ′‐di‐Cbz‐S‐methylisothiourea hydrogenolysis provided effective practical method for synthesis optically ‐methyl‐arginine.