A5100396178- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Alzheimer's disease research and treatments
- Carbohydrate Chemistry and Synthesis
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Proteoglycans and glycosaminoglycans research
- Crystallography and molecular interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- T-cell and B-cell Immunology
- Inflammatory mediators and NSAID effects
- Cancer Cells and Metastasis
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Enzyme Production and Characterization
- Cholinesterase and Neurodegenerative Diseases
- Reproductive System and Pregnancy
- Ubiquitin and proteasome pathways
- Neuroscience and Neuropharmacology Research
- vaccines and immunoinformatics approaches
Shanghai University of Sport
2023-2025
The Ohio State University
1995-2025
Huazhong University of Science and Technology
2010-2025
Xian Central Hospital
2025
Duke University
2022-2025
University of Alberta
2013-2024
Capital Medical University
2016-2024
Tianjin Medical University Cancer Institute and Hospital
2008-2024
National Clinical Research Center for Digestive Diseases
2024
Hebei North University
2022-2024
Claudin18.2 (CLDN18.2), a gastric-specific membrane protein, has been regarded as potential therapeutic target for gastric cancer and other types. The aim of our study was to elucidate whether chimeric antigen receptor T (CAR T) cells redirected CLDN18.2 have the be used in treatment this deadly disease. CLDN18.2-specific humanized antibodies were developed using hybridoma humanization technology. CAR prepared by lentiviral vector transduction. In vitro antitumor activities cytokine...
The claudin 18.2 (CLDN18.2) antigen is frequently expressed in malignant tumors, including pancreatic ductal adenocarcinoma (PDAC). Although CLDN18.2-targeted CAR-T cells demonstrated some therapeutic efficacy PDAC patients, further improvement needed. One of the major obstacles might be abundant cancer-associated fibroblasts (CAFs) tumor microenvironment (TME). Targeting fibroblast activation protein (FAP), a vital characteristic CAFs provides potential way to overcome this obstacle. In...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolid-Phase Chemical-Enzymic Synthesis of Glycopeptides and OligosaccharidesMatthias Schuster, Peng Wang, James C. Paulson, Chi-Huey WongCite this: J. Am. Chem. Soc. 1994, 116, 3, 1135–1136Publication Date (Print):February 1, 1994Publication History Published online1 May 2002Published inissue 1 February 1994https://doi.org/10.1021/ja00082a047RIGHTS & PERMISSIONSArticle Views839Altmetric-Citations194LEARN ABOUT THESE METRICSArticle Views are the...
Genetic, clinical, histopathological and biomarker data strongly support Beta-amyloid (Aβ) induced spreading of Tau-pathology beyond entorhinal cortex (EC), as a crucial process in conversion from preclinical cognitively normal to Alzheimer's Disease (AD), while the underlying mechanism remains unclear. In vivo models have reproducibly recapitulated Aβ-induced Tau-pathology. Tau pathology was thereby also by aggregated Aβ, functionally connected brain areas, reminiscent prion-like seeding...
Prion-like seeding and propagation of Tau-pathology have been demonstrated experimentally may underlie the stereotyped progression neurodegenerative Tauopathies. However, involvement templated misfolding Tau in neuronal network dysfunction behavioral outcomes remains to be explored detail. Here we analyzed repercussions prion-like spreading via connections on function TauP301S transgenic mice. Spontaneous GABAAR-antagonist-induced activity were affected following Tau-misfolding using...
Abstract Turn-on fluorescence imaging is routinely studied; however, turn-on chemiluminescence has been rarely explored for in vivo imaging. Herein, we report the design and validation of probe ADLumin-1 as a amyloid beta (Aβ) species. Two-photon indicates that can efficiently cross blood–brain barrier provides excellent contrast Aβ plaques cerebral angiopathy. In brain shows signal from 5-month-old transgenic 5xFAD mice 1.80-fold higher than age-matched wild-type mice. Moreover, demonstrate...
Abstract Microtubule-associated protein Tau can form aggregates transmissible within the brain, correlating with progression of tauopathies in humans. The transmission requires neuron-released to interact surface receptors on target cells. However, underlying molecular mechanisms astrocytes and downstream effects are unclear. Here, using a spatially resolved proteomic mapping strategy, we show that integrin αV/β1 receptor binds recombinant human Tau, mediating entry fibrils astrocytes....
Abstract Macrophages are among the most abundant immune cells in colorectal cancer (CRC). Re-educating tumor-associated macrophages (TAMs) to switch from protumoral anti-tumoral activity is an attractive treatment strategy that warrants further investigation. However, little known about key pathway activated TAMs. In this study, infitrating CD206 + TAMs CRC were sorted and subjected RNA-seq analysis. Differentially expressed genes found be enriched unfolded protein response/endoplasmic...
Recent evidence has highlighted the anti-inflammatory properties of constituent Green Tea Polyphenols (GTP), epigallocatechin-3-gallate (EGCG) which been suggested to exert a neuroprotective effect on Alzheimer's disease (AD). The current study aimed elucidate EGCG memory function in rats with AD.AD rat models were initially established through an injection Aβ 25-35 solution, followed by gavage at varying doses determine learning and cognitive deficits AD. Morris water maze test was...
To test the utility of "A/T/N" system in Chinese population, we study core Alzheimer's disease (AD) biomarkers a newly established cohort.
O-GlcNAcylation is an abundant posttranslational modification in the brain implicated human neurodegenerative diseases. We have exploited viable null alleles of enzymes O-GlcNAc cycling to examine role well-characterized Caenorhabditis elegans models proteotoxicity. dramatically modulated severity phenotype transgenic tauopathy, amyloid β-peptide, and polyglutamine expansion. Intriguingly, loss function transferase alleviated, whereas O-GlcNAcase enhanced, multiple disease models. The...
Nutrient-driven O-GlcNAcylation is strikingly abundant in the brain and has been linked to development neurodegenerative disease. We selectively targeted O-GlcNAcase (Oga) gene mouse define role of O-GlcNAc cycling central nervous system. Brain knockout animals exhibited dramatically increased levels pleiotropic phenotypes, including early-onset obesity, growth defects, metabolic dysregulation. Anatomical defects Oga included delayed differentiation neurogenesis as well abnormal...
Abstract Anxiety is common in patients suffering from chronic pain. Here, we report anxiety-like behaviors mouse models of pain and reveal that nNOS-expressing neurons ventromedial prefrontal cortex (vmPFC) are essential for pain-induced anxiety but not algesia, using optogenetic chemogenetic strategies. Additionally, determined excitatory projections the posterior subregion paraventricular thalamic nucleus (pPVT) provide a neuronal input drives activation vmPFC our models. Our results...
Abstract CD22, a member of Siglec family sialic acid binding proteins, has restricted expression on B cells. Antibody‐based agents targeting CD22 or CD20 lymphoma and leukemia cells exhibit clinical efficacy for treating these malignancies, but also attack normal leading to immune deficiency. Here, we report chemoenzymatic glycocalyx editing strategy introduce high‐affinity specific ligands onto NK‐92MI cytokine‐induced natural killer achieve tumor‐specific targeting. These CD22‐ligand...
Abstract With the infection rate of Bordetella pertussis at a 60‐year high, there is an urgent need for new anti‐pertussis vaccines. The lipopolysaccharide (LPS) B. attractive antigen vaccine development. presence multiple rare sugars and unusual glycosyl linkages, LPS highly challenging synthetic target. In this work, aided by molecular dynamics simulation modeling, pertussis‐LPS‐like pentasaccharide was chemically synthesized first time. conjugated with powerful carrier, bacteriophage Qβ,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnzymic synthesis of N- and O-linked glycopeptidesChi Huey Wong, Matthias Schuster, Peng Wang, Pamela SearsCite this: J. Am. Chem. Soc. 1993, 115, 14, 5893–5901Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/ja00067a001https://doi.org/10.1021/ja00067a001research-articleACS PublicationsRequest reuse permissionsArticle Views359Altmetric-Citations80LEARN...
Heparan sulfates are implicated in a wide range of biological processes. A major challenge deciphering their structure and activity relationship is the synthetic difficulties to access diverse heparan sulfate oligosaccharides with well-defined sulfation patterns. In order expedite synthesis, divergent strategy was developed. By integrating chemical synthesis two types O-sulfo transferases, seven different hexasaccharides were obtained from single hexasaccharide precursor. This approach...
Elderly populations are susceptible to critical limb ischemia (CLI), but conventional treatments cannot significantly decrease amputation and mortality. Although exercise is an effective "non-pharmacological medicine" targeting mitochondria improve skeletal muscle function, few studies have focused on the application of in CLI.Elderly male C57BL/6 mice (14 months old) were used establish a CLI model assess effect perfusion, performance recovery, apoptosis, mitochondrial turnover...
Accumulating evidence points to a critical role of the brain gut axis as an important paradigm for many central nervous system diseases. Recent studies suggest that propolis has obvious neuroprotective properties and functionality in regulating intestinal bacteria flora, hinting at potential key effect both terminals this regulation. However, currently no clear confirms effects on alcohol-induced depression. Here, we establish alcoholic depression model with C57BL/6J mice demonstrate...