A5005615806- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Multiple Myeloma Research and Treatments
- Immune Response and Inflammation
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Inflammasome and immune disorders
- Monoclonal and Polyclonal Antibodies Research
- Redox biology and oxidative stress
- Cell Adhesion Molecules Research
- Diabetes and associated disorders
- Glycosylation and Glycoproteins Research
- Kruppel-like factors research
- Chronic Lymphocytic Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Xenotransplantation and immune response
- Immune cells in cancer
- Inflammatory Bowel Disease
- Myeloproliferative Neoplasms: Diagnosis and Treatment
QIMR Berghofer Medical Research Institute
2013-2022
Royal Prince Alfred Hospital
2018-2021
The University of Queensland
2011-2020
Concord Repatriation General Hospital
2020
Mater Research
2003-2017
Translational Research Institute
2014-2017
Mater Misericordiae Hospital
2000-2003
Westmead Hospital
2003
Princess Alexandra Hospital
2003
Christchurch Hospital
1997-2002
Oncogenic JAK mutation sensitizes myeloproliferative neoplasms to immune checkpoint inhibition.
Dichloroacetate (DCA), through the inhibition of aerobic glycolysis (the 'Warburg effect') and promotion pyruvate oxidation, induces growth reduction in many tumours is now undergoing several clinical trials. If active multiple myeloma (MM) cells, it can be potentially targeted by DCA to induce inhibition. Representative cell lines a myeloma-bearing mice were treated with DCA, alone combination bortezomib. We found that occurs approximately half MM examined, producing on average 1.86-fold...
Type 1 regulatory T (TR1) cells are Foxp3- interleukin-10 (IL-10)-producing CD4+ with potent immunosuppressive properties, but their requirements for lineage development have remained elusive. We show that TR1 constitute the most abundant population after allogeneic bone marrow transplantation (BMT), express transcription factor Eomesodermin (Eomes), and critical prevention of graft-versus-host disease. demonstrate Eomes is required cell differentiation, during which it acts in concert B...
Multiple myeloma (MM) is an age-dependent hematological malignancy. Evaluation of immune interactions that drive MM relies on in vitro experiments do not reflect the complex cellular stroma involved pathogenesis. Here we used Vk*MYC transgenic mice, which spontaneously develop MM, and demonstrated system plays a critical role control progression response to treatment. We monitored mice had been crossed with Cd226 mutant over period 3 years found CD226 limits spontaneous development. The...
Dendritic cells (DC) are potent antigen-presenting and understanding their mechanisms of antigen uptake is important for loading DC with immunotherapy. The multilectin receptors, DEC-205 macrophage mannose receptor (MMR), potential antigen-uptake receptors; therefore, we examined expression FITC–dextran by various human preparations. RT-PCR analysis detected low levels mRNA in immature blood DC, Langerhans (LC) monocyte-derived (Mo-DC). Its increased markedly upon activation, indicating that...
DEC-205 (CD205) belongs to the macrophage mannose receptor family of C-type lectin endocytic receptors and behaves as an antigen uptake/processing for dendritic cells (DC). To investigate tissue distribution in human leukocytes, we generated a series anti-human monoclonal antibodies (MMRI-5, 6 7), which recognized epitopes within lectin-like domains 1 2, MMRI-7 immunoprecipitated single ∼200 kDa band, identified by mass spectrometry. another mAb (MG38), epitope cysteine-rich fibronectin type...
We established a humanized mouse model incorporating FLT3-ligand (FLT3-L) administration after hematopoietic cell reconstitution to investigate expansion, phenotype, and function of human dendritic cells (DC). FLT3-L increased numbers CD141(+) DC, CD1c(+) and, lesser extent, plasmacytoid DC (pDC) in the blood, spleen, bone marrow mice. subsets were expanded similar degree blood with bias toward expansion subset marrow. Importantly, generated treatment mice are phenotypically functionally...
The primacy of the gastrointestinal (GI) tract in dictating outcome graft-versus-host disease (GVHD) is broadly accepted; however, mechanisms controlling this effect are poorly understood. Here, we demonstrate that GVHD markedly enhances alloantigen presentation within mesenteric lymph nodes (mLNs), mediated by donor CD103+CD11b− dendritic cells (DCs) migrate from colon under influence CCR7. Expansion and differentiation T specifically mLNs driven profound levels alloantigen, IL-12, IL-6...
// Prahlad V. Raninga 1,2 , Giovanna Di Trapani 1 Slavica Vuckovic 3 Maneet Bhatia and Kathryn F. Tonissen School of Natural Sciences, Griffith University, Nathan, QLD, Australia 2 Eskitis Institute for Drug Discovery, QIMR Berghofer Medical Research Institute, Brisbane, Correspondence to: Tonissen, email: Keywords : thioredoxin, multiple myeloma, apoptosis, NF-kB, chemoresistance Received February 10, 2015 Accepted March Published April 12, Abstract Multiple myeloma (MM) is a hematological...
Multiple myeloma (MM) is a B-cell malignancy characterized by an accumulation of abnormal clonal plasma cells in the bone marrow. Introduction proteasome-inhibitor bortezomib has improved MM prognosis and survival; however hypoxia-induced or acquired resistance remains clinical problem. This study highlighted role thioredoxin reductase 1 (TrxR1) MM. Higher TrxR1 gene expression correlated with high-risk disease, adverse overall survival, poor patients. We demonstrated that hypoxia induced...
Transplantation with autologous hematopoietic progenitors remains an important consolidation treatment for patients multiple myeloma (MM) and is thought to prolong the disease plateau phase by providing intensive cytoreduction. However, transplantation induces inflammation in context of profound lymphodepletion that may cause hitherto unexpected immunological effects. We developed preclinical models bone marrow (BMT) MM using Vk*MYC myeloma-bearing recipient mice donor were naive or...
Multiple myeloma (MM) is characterized by an accumulation of abnormal clonal plasma cells in the bone marrow. Despite recent advancements anti-myeloma therapies, MM remains incurable disease. Antioxidant molecules are upregulated many cancers, correlating with tumor proliferation, survival, and chemoresistance therefore, have been suggested as potential therapeutic targets. This study investigated cross-talk between two antioxidant molecules, thioredoxin reductase (TrxR) heme oxygenase-1...
Dendritic cells (DC) are the only antigen-presenting that can activate naïve T lymphocytes and initiate a primary immune response. They also thought to have role in tolerance. DC traffic from blood peripheral tissue where they become activated. then present antigen costimulating signals necessary an In this study, we investigated number, subsets, activation pattern of circulating intestinal patients with clinically mild ulcerative colitis (UC) or Crohn's disease.Patients were recruited, if...
Abstract The majority of allogeneic stem cell transplants are currently undertaken using G-CSF mobilized peripheral blood cells. has diverse biological effects on a broad range cells and IL-10 is key regulator many these effects. Using mixed radiation chimeras in which the hematopoietic or nonhematopoietic compartments were wild-type, IL-10−/−, G-CSFR−/−, combinations thereof we demonstrated that attenuation alloreactive T responses after mobilization required direct signaling by both IL-10....