A5082974246- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- MicroRNA in disease regulation
- Immune cells in cancer
- Inflammasome and immune disorders
- Autoimmune and Inflammatory Disorders Research
- Immune Response and Inflammation
- Adrenal Hormones and Disorders
- IL-33, ST2, and ILC Pathways
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- Gastrointestinal motility and disorders
- Gut microbiota and health
- Tryptophan and brain disorders
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
University of Cambridge
2023-2025
KU Leuven
2015-2024
Babraham Institute
2019-2024
VIB-KU Leuven Center for Brain & Disease Research
2017-2024
Vlaams Instituut voor Biotechnologie
2009-2022
VIB-KU Leuven Center for Microbiology
2010-2019
VIB-KU Leuven Center for Cancer Biology
2013-2017
Rega Institute for Medical Research
2015
VIB-UAntwerp Center for Molecular Neurology
2014
The University of Adelaide
2009
Murine models are a crucial component of gut microbiome research. Unfortunately, multitude genetic backgrounds and experimental setups, together with inter-individual variation, complicates cross-study comparisons global understanding the mouse microbiota landscape. Here, we investigate variability healthy five common lab strains using 16S rDNA pyrosequencing.We find initial evidence for richness-driven, strain-independent murine enterotypes that show striking resemblance to those in human,...
Regulatory T (T reg) cells are indispensable for preventing autoimmunity. Incumbent to this role is the ability of reg exert their suppressor function under inflammatory conditions. We found that cell–mediated tolerance critically dependent on Dicer-controlled microRNA (miRNA) pathway. Depletion miRNA within cell lineage resulted in fatal autoimmunity indistinguishable from cell–deficient mice. In disease-free mice lacking Dicer all or harboring both Dicer-deficient and -sufficient cells,...
Abstract How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild 26 critical in comparison BALs non-COVID-19 pneumonia normal lung. We use pseudotime inference build T-cell monocyte-to-macrophage trajectories model gene expression changes along them. In COVID-19, CD8 + resident-memory (T RM ) CD4...
A mutation in pyrin that disrupts regulation leads to autoinflammatory disease.
The brain is a site of relative immune privilege. Although CD4 T cells have been reported in the central nervous system, their presence healthy remains controversial, and function largely unknown. We used combination imaging, single cell, surgical approaches to identify CD69+ cell population both mouse human brain, distinct from circulating cells. brain-resident was derived through situ differentiation activated circulatory shaped by self-antigen peripheral microbiome. Single-cell sequencing...
Epidemiological and clinical reports indicate that SARS-CoV-2 virulence hinges upon the triggering of an aberrant host immune response, more so than on direct virus-induced cellular damage. To elucidate immunopathology underlying COVID-19 severity, we perform cytokine multiplex profiling in patients. We show hypercytokinemia differs from interferon-gamma-driven storm macrophage activation syndrome, is pronounced critical versus mild-moderate COVID-19. Systems modelling levels paired with...
The ability of immune-modulating biologics to prevent and reverse pathology has transformed recent clinical practice. Full utility in the neuroinflammation space, however, requires identification both effective targets for local immune modulation a delivery system capable crossing blood-brain barrier. characterization small population regulatory T (Treg) cells resident brain presents one such potential therapeutic target. Here, we identified interleukin 2 (IL-2) levels as limiting factor...
The lack of T cell infiltrates is a major obstacle to effective immunotherapy in cancer. Conversely, the formation tumor-associated tertiary-lymphoid-like structures (TA-TLLSs), which are local site humoral and cellular immune responses against cancers, associated with good prognosis, they have recently been detected checkpoint blockade (ICB)-responding patients. However, how these lymphoid aggregates develop remains poorly understood. By employing single-cell transcriptomics, endothelial...
Clinically relevant immunological biomarkers that discriminate between diverse hypofunctional states of tumor-associated CD8 + T cells remain disputed. Using multiomics analysis cell features across multiple patient cohorts and tumor types, we identified niche–dependent exhausted other types states. in “supportive” niches, like melanoma or lung cancer, exhibited reactivity–driven exhaustion (CD8 EX ). These included a proficient effector memory phenotype, an expanded receptor (TCR)...
The tissues are the site of many important immunological reactions, yet how immune system is controlled at these sites remains opaque. Recent studies have identified Foxp3
Inactivation of the autoimmune regulator (Aire) gene causes a rare recessive disorder, polyendocrine syndrome 1 (APS1), but it is not known if Aire-dependent tolerance mechanisms are susceptible to quantitative genetic changes thought underlie more common diseases. In mice with targeted mutation, complete loss Aire abolished expression an insulin promoter transgene in thymic epithelium, had no effect pancreatic islets or testes. Loss one copy diminished endogenous and transgene, resulting...
Regulatory Foxp3(+) T cells (T(R)) are indispensable for preventing autoimmune pathology in multiple organs and tissues. During thymic differentiation cell receptor (TCR)-ligand interactions within a certain increased affinity range, conjunction with gammac-containing cytokine signals, induce Foxp3 expression thereby commit developing thymocytes to the T(R) lineage. The contribution of distinct MHC class II-expressing accessory types process remains controversial, because unique role this...