Yannick Van Herck
A5037605420- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- CAR-T cell therapy research
- Long-Term Effects of COVID-19
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- Adenosine and Purinergic Signaling
- Phagocytosis and Immune Regulation
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Cutaneous Melanoma Detection and Management
- SARS-CoV-2 and COVID-19 Research
- Glioma Diagnosis and Treatment
- Sepsis Diagnosis and Treatment
- T-cell and B-cell Immunology
- Axon Guidance and Neuronal Signaling
- Melanoma and MAPK Pathways
- Renal Transplantation Outcomes and Treatments
- Advanced Breast Cancer Therapies
- 3D Printing in Biomedical Research
Universitair Ziekenhuis Leuven
2020-2025
KU Leuven
2018-2024
VIB-KU Leuven Center for Cancer Biology
2020
Abstract How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild 26 critical in comparison BALs non-COVID-19 pneumonia normal lung. We use pseudotime inference build T-cell monocyte-to-macrophage trajectories model gene expression changes along them. In COVID-19, CD8 + resident-memory (T RM ) CD4...
Epidemiological and clinical reports indicate that SARS-CoV-2 virulence hinges upon the triggering of an aberrant host immune response, more so than on direct virus-induced cellular damage. To elucidate immunopathology underlying COVID-19 severity, we perform cytokine multiplex profiling in patients. We show hypercytokinemia differs from interferon-gamma-driven storm macrophage activation syndrome, is pronounced critical versus mild-moderate COVID-19. Systems modelling levels paired with...
To better understand intrinsic resistance to immune checkpoint blockade (ICB), we established a comprehensive view of the cellular architecture treatment-naive melanoma ecosystem and studied its evolution under ICB. Using single-cell, spatial multi-omics, showed that tumor microenvironment promotes emergence complex transcriptomic landscape. Melanoma cells harboring mesenchymal-like (MES) state, population known confer targeted therapy, were significantly enriched in early on-treatment...
Abstract Rejection remains the main cause of premature graft loss after kidney transplantation, despite use potent immunosuppression. This highlights need to better understand composition and cell-to-cell interactions alloreactive inflammatory infiltrate. Here, we performed droplet-based single-cell RNA sequencing 35,152 transcriptomes from 16 transplant biopsies with varying phenotypes severities rejection without rejection, identified cell-type specific gene expression signatures for...
Clinically relevant immunological biomarkers that discriminate between diverse hypofunctional states of tumor-associated CD8 + T cells remain disputed. Using multiomics analysis cell features across multiple patient cohorts and tumor types, we identified niche–dependent exhausted other types states. in “supportive” niches, like melanoma or lung cancer, exhibited reactivity–driven exhaustion (CD8 EX ). These included a proficient effector memory phenotype, an expanded receptor (TCR)...
Understanding the pathology of COVID-19 is a global research priority. Early evidence suggests that respiratory microbiome may be playing role in disease progression, yet current studies report contradictory results. Here, we examine potential confounders by analyzing upper (n = 58) and lower 35) tract well-phenotyped patients controls combining sequencing, viral load determination, immunoprofiling. We find time intensive care unit type oxygen support, as well associated treatments such...
Abstract Objectives The pandemic spread of the coronavirus SARS‐CoV‐2 is due, in part, to immunological properties host–virus interaction. clinical presentation varies from individual individual, with asymptomatic carriers, mild‐to‐moderate‐presenting patients and severely affected patients. Variation immune response may underlie this variation. Methods Using a high‐dimensional systems immunology platform, we have analysed peripheral blood compartment 6 healthy individuals, 23...
Abstract While immune checkpoint–based immunotherapy (ICI) shows promising clinical results in patients with cancer, only a subset of responds favorably. Response to ICI is dictated by complex networks cellular interactions between malignant and nonmalignant cells. Although insights into the mechanisms that modulate pivotal antitumoral activity cytotoxic T cells (Tcy) have recently been gained, much what has learned based on single-cell analyses dissociated tumor samples, resulting lack...
In melanoma, the lymphocytic infiltrate is a prognostic parameter classified morphologically into ‘brisk’, ‘non-brisk’ and ‘absent’ entailing functional association that has never been proved. Recently, it shown populations can be very heterogeneous, anti-PD-1 immunotherapy supports activated T cells. Here, we characterize immune landscape in primary melanoma by high-dimensional single-cell multiplex analysis tissue sections (MILAN technique) followed image analysis, RT-PCR shotgun...
Since the first outbreak in Hubei, China December 2019, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has rapidly spread across globe. The broadly prescribed anti-CD20 monoclonal antibody rituximab induces B-cell depletion and may affect anti-viral immunity, including development of SARS-CoV-2 antibodies, risk re-infection, impaired vaccine efficacy.1 Recently, Hueso et al.2 reported on protracted coronavirus disease 2019 (COVID-19) patients with profound lymphopenia. In their...
Abstract Objectives Emerging evidence of dysregulation the myeloid cell compartment urges investigations on neutrophil characteristics in coronavirus disease 2019 (COVID‐19). We isolated neutrophils from blood COVID‐19 patients receiving general ward care and hospitalised at intensive units (ICUs) to explore kinetics circulating factors important for migration activation. Methods Multicolour flow cytometry was exploited analysis differentiation activation markers. Multiplex ELISA...
COVID-19 is characterised by a broad spectrum of clinical and pathological features. Natural killer (NK) cells play an important role in innate immune responses to viral infections. Here, we analysed the phenotype activity NK blood patients using flow cytometry, single-cell RNA-sequencing (scRNA-seq), cytotoxic killing assay. In plasma patients, quantified main cytokines chemokines. Our cohort comprises hospitalised low-care ward unit (WARD), with severe disease symptoms intensive care units...
A bstract The stromal compartment of the tumour microenvironment consists a heterogeneous set tissue-resident and tumour-infiltrating cells, which are profoundly moulded by cancer cells. An outstanding question is to what extent this heterogeneity similar between cancers affecting different organs. Here, we profile 233,591 single cells from patients with lung, colorectal, ovary breast (n=36) construct pan-cancer blueprint cell using single-cell RNA protein-based technologies. We identify 68...
Immunotherapies, in particular immune checkpoint blockade (ICB), have improved the clinical outcome of cancer patients, although many fail to mount a durable response. Several resistance mechanisms been identified, but our understanding requirements for robust ICB response is incomplete. We engineered an MHC I/antigen: TCR-matched panel human NSCLC and T cells identify tumor cell-intrinsic cell mechanisms. The top differentially expressed gene resistant was SERPINB9. This serine protease...
Abstract Glioblastoma remains a highly malignant, inevitably recurring brain tumor with dismal prognosis. Tumor progression is influenced by cellular interactions between the and its microenvironment, yet quantitative data on these interactions, particularly under standard of care therapy, sparse. We utilized spatial, single-cell, multiome profiling to map therapy-induced changes in architecture >700 paired glioblastoma samples derived from 101 patients at diagnosis recurrence. This...
Abstract Corona virus disease 2019 (COVID-19) has been associated with a wide range of divergent pathologies, and risk severe is reported to be increased by similarly broad co-morbidities. The present study investigated blood metabolites in order elucidate how infection acute respiratory syndrome coronavirus 2 can lead such variety pathologies what common ground they share. COVID-19 patient samples were taken at hospital admission two Belgian cohorts, third cohort that included longitudinal...
ABSTRACT How innate and adaptive lung immune responses to SARS-CoV-2 synchronize during COVID-19 pneumonitis regulate disease severity is poorly established. To address this, we applied single-cell profiling bronchoalveolar lavages from 44 patients with mild or critical versus non-COVID-19 pneumonia as control. Viral RNA-tracking delineated the infection phenotype epithelial cells, but positioned mainly neutrophils at forefront of viral clearance activity COVID-19. In disease, could execute...
Abstract Primary resistance drastically limits the clinical success of immune checkpoint blockade (ICB) in melanoma. Resistance to ICB may also develop when tumours relapse after targeted therapy. To identify cancer cell-intrinsic mechanisms driving ICB, we generated single-cell RNA-sequencing (scRNA-seq) data from a prospective longitudinal cohort patients on therapy, including an early time point obtained only one cycle treatment. Comparing these with murine scRNA-seq datasets, established...
Abstract Cytotoxic T cell (CTL) infiltration of the tumor carries potential to limit cancer progression, but their exclusion by immunosuppressive microenvironment hampers efficiency immunotherapy. Here, we show that expression axon guidance molecule Plexin-A4 (Plxna4) in CTLs, especially effector/memory CD8+ cells, is induced upon T-cell activation, sustained circulation, reduced when entering bed. Therefore, deleted Plxna4 and observed Plxna4-deficient CTLs acquired improved homing capacity...