A5018706163- Congenital heart defects research
- Renal and related cancers
- Coronary Artery Anomalies
- Ichthyology and Marine Biology
- Tissue Engineering and Regenerative Medicine
- Physiological and biochemical adaptations
- Angiogenesis and VEGF in Cancer
- Congenital Heart Disease Studies
- Zebrafish Biomedical Research Applications
- Fish Biology and Ecology Studies
- Pancreatic function and diabetes
- Congenital Diaphragmatic Hernia Studies
- Fish biology, ecology, and behavior
- Liver physiology and pathology
- Cardiac Valve Diseases and Treatments
- Medical research and treatments
- Educational theories and practices
- Cardiac Fibrosis and Remodeling
- Parasite Biology and Host Interactions
- Aquaculture Nutrition and Growth
- Congenital Anomalies and Fetal Surgery
- Developmental Biology and Gene Regulation
- Psychology Research and Bibliometrics
- Neurobiology and Insect Physiology Research
- Aortic Disease and Treatment Approaches
Universidad de Málaga
2016-2025
Andalusian Centre for Nanomedicine and Biotechnology
2015-2021
Instituto de Investigación Biomédica de Málaga
2016-2021
MRC Human Genetics Unit
2009
Institute of Genetics and Cancer
2009
Lund University
2002
Universitat de València
2002
Institut de Biologia Evolutiva
1998
University of Salford
1995
Epicardial-derived signals are key regulators of cardiac embryonic development. An important part these is known to relate a retinoic acid (RA) receptor-dependent mechanism. RA potent morphogen synthesised by Raldh enzymes, Raldh2 being the predominant one in mesodermal tissues. Despite importance epicardial retinoid signalling heart, molecular mechanisms controlling transcription remain unknown. In current study, we show that Wt1-null cells display decreased expression both vivo and vitro....
Significance Here we show, for the first time to our knowledge, that septum transversum/proepicardium (ST/PE)-derived endothelial cells are required proper coronary blood vessel morphogenesis. We used different mouse transgenic lines show ST/PE contributes endothelium and endocardium is not only developmental origin of this tissue. Our results indicate ST/PE-derived preferentially incorporate into prospective arteries capillaries but veins. Deletion epicardial regulator Wilms’ tumor...
Perlecan is a heparan-sulfate proteoglycan abundantly expressed in pericellular matrices and basement membranes during development. Inactivation of the perlecan gene mice lethal at two developmental stages: around E10 birth. We report high incidence malformations cardiac outflow tract perlecan-deficient embryos. Complete transposition great arteries was diagnosed 11 out 15 late embryos studied (73%). Three these also showed semilunar valves. Mesenchymal cells were abnormally abundant mutant...
A study about the hypothetical contribution of epicardial cells to subepicardial mesenchyme was carried out in Syrian hamster embryos 9–12 days post coitum (dpc) and chick 3–5 incubation. In epicardium subepicardium these we have immunolocated proteins cytokeratin (CK), vimentin (VIM), fibronectin (FN), two antigens related transformation endocardial into valvuloseptal mesenchyme, ES/130 JB3. embryos, CK+ mesenchymal (SEMC) were apparently migrating from primitive 9.5 dpc at atrioventricular...
(+)-aeroplysinin-1, an antibacterial brominated compound produced by certain sponges, was selected during a blind high-throughput screening for new potential antiangiogenic compounds obtained from marine organisms. In variety of experimental systems, representing the sequential events angiogenic process, aeroplysinin-1 treatment endothelial cells resulted in strong inhibitory effects. Aeroplysinin-1 inhibited growth culture and induced cell apoptosis. Capillary tube formation on Matrigel...
Abstract Coronary vessel formation is a special case in the context of embryonic vascular development. A major part coronary cellular precursors (endothelial, smooth muscle, and fibroblastic cells) derive from proepicardium epicardium what can be regarded as late event angioblastic muscle cell differentiation. Thus, morphogenesis dependent on epithelial–mesenchymal transformation epicardium. In this study, we present several novel observations about process vasculogenesis avian embryos,...
Lungs develop from paired endodermal outgrowths surrounded by a mesodermal mesenchyme. Part of this mesenchyme arises epithelial-mesenchymal transition the mesothelium that lines pulmonary buds. Previous studies have shown mesothelium-derived contributes to smooth muscle vessels, but its significance for lung morphogenesis and developmental fate are still little known. We studied issue using transgenic mouse model mWt1/IRES/GFP-Cre (Wt1 cre ) crossed with Rosa26R-EYFP reporter mouse. In...
The zinc finger transcription factor GATA4 controls specification and differentiation of multiple cell types during embryonic development. In mouse liver, Gata4 is expressed in the endodermal hepatic bud adjacent mesenchyme septum transversum. Previous studies have shown that inactivation impairs liver formation. However, whether these defects are caused by loss endoderm or transversum remains to be determined. this study, we investigated role mesenchymal activity We conditionally...
Congenital diaphragmatic hernia (CDH) is a severe birth defect. Wt1-null mouse embryos develop CDH but the mechanisms regulated by WT1 are unknown. We have generated murine model with conditional deletion of in lateral plate mesoderm, using G2 enhancer Gata4 gene as driver. 80% G2- Cre ; Wt1 fl/fl developed typical Bochdalek-type CDH. show that posthepatic mesenchymal coelomic epithelium gives rise to mesenchyme populates pleuroperitoneal folds isolating pleural cavities before migration...
In response to liver injury, hepatic stellate cells activate and acquire proliferative contractile features. The regression of fibrosis appears involve the clearance activated cells, either by apoptosis or reversion toward a quiescent-like state, process called deactivation. Thus, deactivation active has emerged as novel promising therapeutic approach for fibrosis. However, our knowledge master regulators involved in and/or activation fibrotic is still limited. transcription factor GATA4...
Background Bicuspid aortic valve is the most frequent congenital cardiac malformation in humans. However, morphogenesis of defect still unknown. Previous work showed that, Syrian hamster, bicuspid valves with sinuses arranged ventrodorsal orientation are expressions a trait variation which takes form continuous phenotypic spectrum, ranging from tricuspid no fusion ventral commissure to devoid any raphe. The present study was designed elucidate mechanism involved formation hamsters as...