A5049512511- Congenital heart defects research
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Barrier Structure and Function Studies
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Congenital Heart Disease Studies
- Cardiovascular Health and Disease Prevention
- Congenital Diaphragmatic Hernia Studies
- Hippo pathway signaling and YAP/TAZ
- ATP Synthase and ATPases Research
- Cancer-related molecular mechanisms research
- Cardiovascular Disease and Adiposity
- Pluripotent Stem Cells Research
- Sexual Differentiation and Disorders
- Neonatal Respiratory Health Research
- Diet and metabolism studies
- Renal and Vascular Pathologies
- TGF-β signaling in diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Cerebrovascular and genetic disorders
Universitat de Barcelona
2014-2024
Institute for Research in Biomedicine
2018-2023
Faculty (United Kingdom)
2015
Western General Hospital
2011
MRC Institute of Genetics and Molecular Medicine
2009-2011
MRC Human Genetics Unit
2009
Mario Negri Institute for Pharmacological Research
2000-2005
University of Insubria
2001
Junctional adhesion molecule (JAM) is an integral membrane protein that has been reported to colocalize with the tight junction molecules occludin, ZO-1, and cingulin. However, evidence for association of JAM these missing. Transfection Chinese hamster ovary cells (either alone or in combination occludin) resulted enhanced junctional localization both endogenous ZO-1 cotransfected occludin. Additionally, was coprecipitated detergent-insoluble fraction Caco-2 epithelial cells. A putative...
Claudin-1 is an integral membrane protein component of tight junctions. The Snail family transcription factors are repressors that play a central role in the epithelial-mesenchymal transition, process occurs during cancer progression. and Slug members direct E-cadherin act by binding to specific E-boxes its proximal promoter. In present study, we demonstrate overexpression or causes decrease transepithelial electrical resistance. Overexpression MDCK (Madin-Darby canine kidney) cells...
Epicardial-derived signals are key regulators of cardiac embryonic development. An important part these is known to relate a retinoic acid (RA) receptor-dependent mechanism. RA potent morphogen synthesised by Raldh enzymes, Raldh2 being the predominant one in mesodermal tissues. Despite importance epicardial retinoid signalling heart, molecular mechanisms controlling transcription remain unknown. In current study, we show that Wt1-null cells display decreased expression both vivo and vitro....
The blood-brain barrier (BBB) ensures the homeostasis of brain microenvironment, mostly through complex tight junctions between endothelial cells that prevent passage hydrophilic molecules from blood to and vice versa. A recent study has shown in vivo systemic administration erythropoietin (Epo) protects against injury. Using an vitro model bovine BBB, we observed expression Epo receptor is modulated by its ligand hypoxic stimuli such as vascular growth factor (VEGF) treatment. In addition,...
TDP-43 is a DNA/RNA-binding protein that regulates gene expression and its malfunction in neurons has been causally associated with multiple neurodegenerative disorders. Although progress made understanding the functions of neurons, little known about role endothelial cells (ECs), angiogenesis vascular function. Using inducible EC-specific knockout mice, we showed required for sprouting angiogenesis, barrier integrity blood vessel stability. Postnatal deletion leaded to retinal...
We report here that junctional adhesion molecule (JAM) interacts with calcium/calmodulin-dependent serine protein kinase (CASK), a related to membrane-associated guanylate kinases. In Caco-2 cells, JAM and CASK were coprecipitated found colocalize at intercellular contacts along the lateral surface of plasma membrane. Association requires PSD95/dlg/ZO-1 (PDZ) domain putative PDZ-binding motif Phe-Leu-Val(COOH) in cytoplasmic tail JAM. Temporal dissociation localization two proteins suggests...
Article23 July 2021Open Access Transparent process Mfn2 localization in the ER is necessary for its bioenergetic function and neuritic development Sergi Casellas-Díaz Department of Cell Biology, Physiology Immunology, Celltec-UB, University Barcelona, Spain Institute Neurosciences, Search more papers by this author Raquel Larramona-Arcas orcid.org/0000-0001-8427-8007 Guillem Riqué-Pujol Paula Tena-Morraja orcid.org/0000-0002-7617-3392 Claudia Müller-Sánchez orcid.org/0000-0002-3654-2683 Marc...
The low number of postnatal endothelial progenitor cells (EPC) in the circulation limits their therapeutic application cardiovascular medicine. Processed lipoaspirate (PLA) differentiate into osteoid, adipose, muscle, and cartilaginous cells. This study examines potential PLA as a source EPCs.PLA obtained from human lipoaspirates were cultured for 1 week serum-depleted medium to form three-dimensional cell clusters (3DCC). phenotype 3DCC-derived was assessed by immunofluorescence staining...
ABSTRACT The epicardium plays a crucial role in embryonic heart development and adult repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of main markers epicardium, is essential for epicardial function. Here, we analyse transcriptomic profile epicardial-enriched cells at different stages from control epicardial-specific Wt1 knockout (Wt1KO) mice. Transcriptomic cell morphology analyses Wt1KO mice revealed defect process mutant including sustained...
The embryonic epicardium is an important source of cardiovascular precursor cells and paracrine factors that are required for adequate heart formation. Signaling pathways regulated by WT1 promote development have started to be described; however, there little information on signaling could act in a negative manner. Transcriptome analysis Wt1KO epicardial reveals unexpected role repressing the expression interferon-regulated genes involved regulation morphogenesis. Here, we showed repress...
Synaptic activity is the main energy-consuming process in central nervous system. We are beginning to understand how energy supplied and used during synaptic by neurons. However, long-term metabolic adaptations associated with a previous episode of not well understood. Herein, we show that an increases mitochondrial bioenergetics beyond duration transcriptionally inducing expression iron metabolism genes consequent enhancement cellular uptake. Iron necessary component electron transport...
Circulating endothelial progenitor cells (EPCs) promote vascular repair and maintain integrity of the monolayer. Reduced EPCs number has been associated with dysfunction in various cardiovascular diseases. Cardiovascular disease risk is higher renal transplant patients (RT) than general population. We studied proliferation RT, examined association other factors such as reduced glomerular filtration rate (GFR) LDL cholesterol. concentration was determined 94 RT 39 control subjects (C) by flow...
Mitochondria morphology constantly changes through fission and fusion processes that regulate mitochondrial function, it therefore plays a prominent role in cellular homeostasis. Cell death progression is associated with fission. Fission mediated by the mainly cytoplasmic Drp1, which activated different post-translational modifications recruited to mitochondria perform its function. Our research other studies have shown early moments of excitotoxic insult Drp1 must be nitrosylated mediate...
Junctional adhesion molecule-A (JAM-A) regulates key inflammatory responses, such as edema formation and leukocyte transmigration. Although it has been reported that the cytokine tumor necrosis factor (TNF) causes disassembly of JAM-A from intercellular junctions, mechanism not elucidated fully. Here, we report TNF enhances solubility in Triton X-100 increases amount Triton-soluble dimers at cell surface but does change total levels cellular JAM-A. Thus hypothesized redistribution junctions...
ABSTRACT Wt1 encodes a zinc finger protein that is crucial for epicardium development. Although WT1 also expressed in coronary endothelial cells (ECs), the abnormal heart development observed knockout mice mainly attributed to its functions epicardium. Here, we have generated an inducible endothelial-specific mouse model (Wt1KOΔEC). Deletion of ECs during plexus formation impaired blood vessels and myocardium RNA-Seq analysis from Wt1KOΔEC demonstrated deletion exerted major impact on...
Transforming growth factor‐β (TGFβ1) is a proangiogenic factor both, in vitro and vivo, that mainly involved the later phases of angiogenesis. In an attempt to identify genes participate this effect, we found TGFβ1 down‐regulates expression adenylate cyclase VI. addition, cAMP analogs (8‐Bromo‐cAMP) forskolin (an activator) also reduced TGFβ1‐induced angiogenesis mouse endothelial cell lines primary cultures human umbilical vein cells on collagen gels. Induction Ets‐1 plasminogen activator...
Background. Hypertension has been related to endothelial dysfunction. Patients with refractory hypertension (RH) have a reduced number of progenitor cells (EPCs). Aim. To evaluate if blood EPC levels relate endothelium‐dependent vasodilation (ED‐VD) in RH. Methods. We analyzed 29 RH confirmed by 24‐h ambulatory pressure monitoring and assessed complete clinical laboratory evaluation. EPCs were isolated from peripheral mononuclear (MNC) flow cytometry. ED‐VD was determined measuring...