A5027524304- Inflammatory mediators and NSAID effects
- RNA Interference and Gene Delivery
- X-ray Diffraction in Crystallography
- Ferrocene Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Crystallization and Solubility Studies
- Chemical Synthesis and Analysis
- Eicosanoids and Hypertension Pharmacology
- Click Chemistry and Applications
- Antimicrobial Peptides and Activities
- Nitric Oxide and Endothelin Effects
- Estrogen and related hormone effects
- Catalytic Cross-Coupling Reactions
- Diabetes Treatment and Management
- Steroid Chemistry and Biochemistry
- Pharmacogenetics and Drug Metabolism
- Metal complexes synthesis and properties
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chronic Kidney Disease and Diabetes
- PI3K/AKT/mTOR signaling in cancer
- Birth, Development, and Health
- Caveolin-1 and cellular processes
- Sulfur-Based Synthesis Techniques
- Clinical Nutrition and Gastroenterology
- Biochemical and Structural Characterization
Universidad de Murcia
2020
Hospital General Universitario Morales Meseguer
2019
University of Girona
2012-2017
Hospital de Sant Pau
2001-2017
Institut d'Investigacions Biomèdiques de Barcelona
2012-2014
Universitat Autònoma de Barcelona
2007
Hospital Del Mar
2004
Puigvert Foundation
2001
Centro de Biología Molecular Severo Ochoa
2000
Abstract —In a previous work, we postulated that endothelial cells possess only the following 2 enzymes involved in prostanoid synthesis: cyclooxygenase and prostacyclin synthase. The present work focused on investigating expression of prostaglandin (PG) E synthase (PGES) vascular cells. After incubation smooth muscle (SMCs) human umbilical vein (HUVECs) with [ 14 C]arachidonic acid, profile synthesis was assessed by HPLC. Untransformed PGH released evaluated as difference formation PGF 2α...
The copper-catalyzed arylation of nucleophiles has been established as an efficient methodology for the formation C–C and C–heteroatom bonds. Considering advances during last two decades, ligand choice plays a key role in such transformations can strongly influence catalytic efficiency. applicability these Ullmann-type coupling reactions regarding orthogonal selectivity different functional groups constitutes challenging subject current synthetic strategies. Herein, we report useful toolkit...
Prostaglandin (PG)E2 is relevant in tumor biology, and interactions between stroma cells dramatically influence progression. We tested the hypothesis that cross-talk head neck squamous cell carcinoma (HNSCC) fibroblasts could substantially enhance PGE2 biosynthesis. observed an enhanced production of cocultures HNSCC lines fibroblasts, which was consistent with upregulation COX-2 microsomal PGE-synthase-1 (mPGES-1) fibroblasts. In cultured endothelial cells, medium from treated...
Unraveling the mechanistic details of copper-catalyzed arylation nucleophiles (Ullmann-type couplings) is a very challenging task. It matter intense debate whether it radical-based process or an organometallic redox-based process. The ancillary ligand choice in Ullmann-type couplings plays key role such transformations and can strongly influence catalytic efficiency as well mechanism. Here, we show how predesigned tridentate pincer-like catalyst undergoes deactivation pathway through...
<bold>BP16</bold>is a non-toxic cell-penetrating peptide with high cellular uptake<italic>in vitro</italic>. This is an efficient vector for the delivery of therapeutic agents into cells, as has been shown anticancer drug chlorambucil.
The conjugation of redox-active complexes that can function as chemical nucleases to cationic tetrapeptides is pursued in this work order explore the expected synergistic effect between these two elements DNA oxidative cleavage. Coordination biologically relevant first row metal ions, such Zn(II) or Cu(II), containing tetradentate ligands 1,4-dimethyl-7-(2-pyridylmethyl)-1,4,7-triazacyclononane (Me2PyTACN) and (2S,2S′)-1,1′-bis(pyrid-2-ylmethyl)-2,2′-bipyrrolidine ((S,S′)-BPBP) have been...
Inflammatory bowel disease (IBD) is associated with a considerable burden to the patient and society. However, current data on IBD incidence are limited because of paucity nationwide epidemiological studies, heterogeneous designs, low number participating centers sample size. The EpidemIBD study large-scale investigation provide an accurate assessment in Spain, as well treatment patterns outcomes.This multicenter, population-based cohort included patients aged >18 years (Crohn's disease,...
There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial (ECs) necessary for the accumulation of PMN around a tumour. This work reports ability two PMN-sensitive, human, permanent cell lines-colorectal adenocarcinoma (HT-29) pharyngeal squamous-cell carcinoma (FaDu) cells-to act as inflammatory foci. PMNs were cytotoxic to both lines, adhesion being important in this effect. The released appreciable...
Abstract A microwave‐assisted solid‐phase Suzuki–Miyaura reaction has been employed for the synthesis of 5‐arylhistidine‐containing peptides. In particular, sequences containing a 5‐arylhistidine at 1‐ or 4‐positions have designed based on lead antimicrobial The cross‐coupling involved arylation resin‐bound 5‐bromohistidine with an arylboronic acid in solution under microwave irradiation. This protocol is compatible common protecting groups used peptide chemistry. resulting biaryl linear...
The undecapeptide KKLFKKILKKL-NH2 (BP16) is a non-toxic cell-penetrating peptide (CPP) that mainly internalized into cancer cells through clathrin dependent endocytic mechanism and localizes in late endosomes. Moreover, this CPP able to enhance the cellular uptake of chlorambucil (CLB) improving its cytotoxicity. In work, we further explored properties BP16 those arginine analogue BP308. We investigated influence on cytotoxicity conjugating CLB at N- or C-terminal end these undecapeptides....
Abstract Prostacyclin ( PGI 2 ) plays a role in cancer progression but the mechanism is currently poorly understood. Additionally, no data are available about prognostic value of pathway head and neck squamous cell carcinoma HNSCC therapy. We evaluated expression patients. production synthase PGIS expression, terms mRNA RT‐PCR protein (immunoblotting), were lower tumour samples than non‐tumoural mucosa, whereas, as expected, COX ‐2 was increased samples. Using local control after...
The purpose of this study is to establish renal resistive index (RRI) normal kidneys in puppies aged from newborn 20 weeks age and determine the at which RRI reaches adult dog values. Six healthy intact beagles six 1 day after birth were used. In dogs, ultrasonographic scans performed once, puppies, studies on first 1, 2, 3, 4, 6, 8, 12, 16, age. was obtained interlobular arcuate arteries each kidney. There no statistical differences between values right left kidney nor intrarenal...
Peptide conjugates incorporating a red-ox active aminopyridine ligand bound to the cell-penetrating peptide <bold>BP16</bold> display high cytotoxicity.
Prostanoids play a critical role in clinical areas such as inflammation, thrombosis, immune response, and cancer. Although some studies suggest that there are genes determine variability of prostanoid-related phenotypes, the genetic influence on these traits has not been evaluated.The relative contributions environmental influences to prostanoid biosynthetic pathway-related cyclooxygenase isoenzymes, microsomal-PGE-synthase-1 TxA-synthase expression, thromboxane-A(2) prostaglandin-E(2)...
The effect of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide (cPTIO), a nitric oxide (NO) scavenger that yields nitrogen dioxide (NO2) in rat endotoxemia model was investigated. Endotoxin (lipopolysaccharide [LPS]) increased NO synthase (NOS) activity and inducible NOS expression measured lung plasma levels nitrite/nitrate, 6-oxo-prostaglandin (PG) F1α, thromboxane B2, PGF2α,. Infusion cPTIO significantly reduced LPS-induced mean arterial blood pressure decline mortality...
Dyslipidemia, metabolic disorders and/or obesity are postulated as risk factors for pancreatic ductal adenocarcinoma (PDAC). The majority of patients with these alterations have low density lipoproteins (LDLs) increased susceptibility to become aggregated in the extracellular matrix (ECM). LDL aggregation can be efficiently inhibited by low-density lipoprotein receptor-related protein 1 (LRP1)-based peptides. objectives this work were: (i) determine if LDLs affect intracellular cholesteryl...
Overnight exposure to interleukin (IL)-1beta caused a dramatic hyporesponsiveness phenylephrine, increased nitric oxide (NO) and prostacyclin production, induced cycloxygenase-2 expression in rat aortic rings. Using different inhibitors, we found that this hyporeactivity was mediated by NO, prostacyclin, activation of charybdotoxin-sensitive K(+) channels. The latter independent the presence endothelium NO prostanoid synthesis during challenge with phenylephrine. Activation channels probably...