A5025560028- Microbial Community Ecology and Physiology
- Marine Toxins and Detection Methods
- Aquatic Ecosystems and Phytoplankton Dynamics
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Marine and coastal ecosystems
- Photosynthetic Processes and Mechanisms
- Biocrusts and Microbial Ecology
- Microscopic Colitis
- Neuroscience and Neuropharmacology Research
- Metabolomics and Mass Spectrometry Studies
- Metal Extraction and Bioleaching
- Minerals Flotation and Separation Techniques
- Freshwater macroinvertebrate diversity and ecology
- Inflammatory Bowel Disease
- Bacteriophages and microbial interactions
- Agricultural and Food Production Studies
- Marine Sponges and Natural Products
- Environmental remediation with nanomaterials
- Neuroendocrine regulation and behavior
- Pesticide and Herbicide Environmental Studies
- Parvovirus B19 Infection Studies
- Bioactive Compounds and Antitumor Agents
- Collagen: Extraction and Characterization
Pontificia Universidad Católica de Chile
2012-2024
Centro Nacional de Biotecnología
2024
Weatherford College
2024
Hospital General Universitario Morales Meseguer
2019
University of Chile
1990-2004
Cyanobacterial morphology is diverse, ranging from unicellular spheres or rods to multicellular structures such as colonies and filaments. Multicellular species represent an evolutionary strategy differentiate compartmentalize certain metabolic functions for reproduction nitrogen (N2) fixation into specialized cell types (e.g. akinetes, heterocysts diazocytes). Only a few filamentous, differentiated cyanobacterial species, with genome sizes over 5 Mb, have been sequenced. We sequenced the...
One of the major problems in production lipids for biotechnological purposes using microalgae is maintaining a high productivity these molecules without reducing cellular biomass. High rates are usually obtained by cultivating under different stress conditions. However, many changes result lower biomass productivity. Therefore, optimization culture conditions and genetic modification techniques organisms needed to generate robust new strains profitable economic use.In this work, we describe...
Different environmental nitrogen sources play selective roles in the development of cyanobacterial blooms and noxious effects are often exacerbated when toxic cyanobacteria dominant. Cylindrospermopsis raciborskii CS-505 (heterocystous, fixing) Raphidiopsis brookii D9 (non-N2 produce nitrogenous toxins cylindrospermopsin (CYN) paralytic shellfish (PSTs), respectively. These toxin groups biosynthesized constitutively by two independent putative gene clusters, whose flanking genes target for...
Composition, carbon and nitrogen uptake, gene transcription of microbial mat communities in Porcelana neutral hot spring (Northern Chilean Patagonia) were analyzed using metagenomics, metatranscriptomics isotopically labeled (H13CO3) (15NH4Cl K15NO3) assimilation rates. The community included 31 phyla, which only Cyanobacteria Chloroflexi dominant. At 58 °C both phyla co-occurred, with similar contributions relative abundances metagenomes total transcriptional activity. 66 °C, filamentous...
Analysis of the 16S rRNA genes retrieved directiy from different environments has proven to be a powerful tool that greatly expanded our knowledge microbial diversity and phylogeny. It is shown here sequence similarity between 80 100% among rDNAs can estimated by electrophoretic migration their heteroduplexes. This was measured hybridization electrophoresis in polyacrylamide gels product obtained after PCR amplification almost entire gene bacterial species. These heteroduplexes were also...
Species of Microcystis are the most common bloom-forming cyanobacteria in several countries. Despite extensive studies regarding production bioactive cyanopeptides this genus, there limited data on isolated strains from Brazil. Three sp. were Salto Grande Reservoir (LTPNA01, 08 and 09) investigated for presence mcy genes, microcystins other cyanopeptides. Microcystin microginin was confirmed two isolates using high-resolution tandem mass spectrometry after electrospray ionization...
The toxin producing nitrogen-fixing heterocystous freshwater cyanobacterium Cylindrospermopsis raciborskii recently radiated from its endemic tropical environment into sub-tropical and temperate regions, a radiation likely to be favored by ability fix dinitrogen (diazotrophy). Although most cyanobacteria differentiate regularly spaced intercalary heterocysts along their trichomes when combined nitrogen sources are depleted, C. differentiates only two terminal (one at each trichome end) that...
Azaspiracids (AZA), a group of lipophilic phycotoxins, are produced by some species the marine dinophycean genus Azadinium. AZA have recently been detected in shellfish from Southeast Pacific, however, AZA-producing not recorded yet area. This study is first record Azadinium and poporum Pacific side South America. Three strains A. Chañaral (Northern Chile) comply to type description presence multiple pyrenoids, thecal plate details, position ventral pore located on left plate. Molecular...
For tolerating extreme desiccation, cyanobacteria are known to produce both compatible solutes at intracellular level and a copious amount of exopolysaccharides as protective coat. However, these molecules make cyanobacterial cells refractory broad spectrum cell disruption methods, hindering genome sequencing, molecular studies. In fact, few genomes already available from extremely desiccated environments such deserts. this work, we report the 5.4 Mbp draft (with 100% completeness in 105...
Cylindrospermopsis raciborskii CS-505 is an invasive freshwater filamentous cyanobacterium that when grown diazotrophically may develop trichomes of up to 100 vegetative cells while differentiating only two end heterocysts, the sole sites for their N2-fixation process. We examined diazotrophic growth and intercellular transfer mechanisms in C. CS-505. Subjecting cultures a combined-nitrogen-free medium elicit N2 fixation, trichome length remained unaffected rates decreased. The structures...
Inflammatory bowel disease (IBD) is associated with a considerable burden to the patient and society. However, current data on IBD incidence are limited because of paucity nationwide epidemiological studies, heterogeneous designs, low number participating centers sample size. The EpidemIBD study large-scale investigation provide an accurate assessment in Spain, as well treatment patterns outcomes.This multicenter, population-based cohort included patients aged >18 years (Crohn's disease,...
Extensive bacterial growth was observed when copper sulfide ores were leached with 0.6 N sulfuric acid. The population developed in this condition examined by characterization of the spacer regions between 16S and 23S rRNA genetic loci obtained after PCR amplification DNA extracted from ore. spacers had sizes found strains "Leptospirillum ferrooxidans" Thiobacillus thiooxidans, except for a larger one, approximately 560 bp long, that not any examined, including those ferrooxidans. bacteria...
Paralytic shellfish poisoning toxins (PSTs) are a family of more than 30 natural alkaloids synthesized by dinoflagellates and cyanobacteria whose toxicity in animals is mediated voltage-gated Na+ channel blocking. The export PST analogues may be through SxtF SxtM, two putative MATE (multidrug toxic compound extrusion) transporters encoded PSTs biosynthetic gene cluster (sxt). sxtM present every sxt analyzed; however, sxtF only the Cylindrospermopsis-Raphidiopsis clade. These energetically...
Background and study aims Chromoendoscopy with targeted biopsy is the technique of choice for colorectal cancer screening in longstanding inflammatory bowel disease. We aimed to analyze results a chromoendoscopy program assess possibility identifying low-risk dysplastic lesions by their endoscopic appearance order avoid histological analysis. Materials methods retrospectively reviewed chromoendoscopies performed between February 2011 June 2017 seven Spanish hospitals standardized fashion....