A5074475274- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Microbial metabolism and enzyme function
- Coral and Marine Ecosystems Studies
- Wastewater Treatment and Nitrogen Removal
- Aquatic Ecosystems and Phytoplankton Dynamics
- Biocrusts and Microbial Ecology
- Anaerobic Digestion and Biogas Production
- Marine and coastal ecosystems
- Marine Toxins and Detection Methods
- Marine Biology and Ecology Research
- Space Science and Extraterrestrial Life
- Dark Matter and Cosmic Phenomena
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Ocean Acidification Effects and Responses
- Alcohol Consumption and Health Effects
- Plant responses to water stress
- Metalloenzymes and iron-sulfur proteins
- Aquaculture disease management and microbiota
- Neuroscience of respiration and sleep
- Marine Bivalve and Aquaculture Studies
San Diego State University
2018-2025
Minot State University
2017-2022
University of California, San Diego
2018
University of Minnesota
2017
University of Concepción
2017
Pontificia Universidad Católica de Chile
2011-2014
High-throughput RNA sequencing offers broad opportunities to explore the Earth virome. Mining 5,150 diverse metatranscriptomes uncovered >2.5 million virus contigs. Analysis of >330,000 RNA-dependent polymerases (RdRPs) shows that this expansion corresponds a 5-fold increase known diversity. Gene content analysis revealed multiple protein domains previously not found in viruses and implicated virus-host interactions. Extended RdRP phylogeny supports monophyly five established phyla reveals...
Metagenomes encode an enormous diversity of proteins, reflecting a multiplicity functions and activities
Species of Microcystis are the most common bloom-forming cyanobacteria in several countries. Despite extensive studies regarding production bioactive cyanopeptides this genus, there limited data on isolated strains from Brazil. Three sp. were Salto Grande Reservoir (LTPNA01, 08 and 09) investigated for presence mcy genes, microcystins other cyanopeptides. Microcystin microginin was confirmed two isolates using high-resolution tandem mass spectrometry after electrospray ionization...
The toxin producing nitrogen-fixing heterocystous freshwater cyanobacterium Cylindrospermopsis raciborskii recently radiated from its endemic tropical environment into sub-tropical and temperate regions, a radiation likely to be favored by ability fix dinitrogen (diazotrophy). Although most cyanobacteria differentiate regularly spaced intercalary heterocysts along their trichomes when combined nitrogen sources are depleted, C. differentiates only two terminal (one at each trichome end) that...
Considering the increasing interest in understanding biotic component of methane removal from our atmosphere, it becomes essential to study physiological characteristics and genomic potential methanotroph isolates, especially their traits allowing them adapt elevated growth temperatures. The genetic signatures Methylocaldum species have been detected many terrestrial aquatic ecosystems. A small set representatives this genus has isolated maintained culture. is commonly described as...
Cylindrospermopsis raciborskii CS-505 is an invasive freshwater filamentous cyanobacterium that when grown diazotrophically may develop trichomes of up to 100 vegetative cells while differentiating only two end heterocysts, the sole sites for their N2-fixation process. We examined diazotrophic growth and intercellular transfer mechanisms in C. CS-505. Subjecting cultures a combined-nitrogen-free medium elicit N2 fixation, trichome length remained unaffected rates decreased. The structures...
Hypersaline environments represent some of the most challenging settings for life on Earth. Extremely halophilic microorganisms have been selected to colonize and thrive in these extreme by virtue a broad spectrum adaptations counter high salinity osmotic stress. Although there is substantial data microbial taxonomic diversity ecosystems their primary osmoadaptation mechanisms, less known about how hypersaline shape genomes inhabitants at functional level. In this study, we analyzed...
Pseudoalteromonas luteoviolacea is a globally distributed marine bacterium that stimulates the metamorphosis of animal larvae, an important bacteria-animal interaction can promote recruitment animals to benthic ecosystems. Recently, different P. isolates have been shown produce two stimulatory factors induce tubeworm and coral metamorphosis; Metamorphosis-Associated Contractile structures (MACs) tetrabromopyrrole (TBP) respectively. However, it remains unclear what proportion possess genes...
ABSTRACT Cáhuil Lagoon in central Chile harbors distinct microbial communities various solar salterns that are arranged as interconnected ponds with increasing salt concentrations. Here, we report the metagenome of 3.0- to 0.2-µm fraction community present a crystallizer pond 34% salinity.
Considering the increasing interest in understanding biotic component of methane removal from our atmosphere, it becomes essential to study physiological characteristics and genomic potential methanotroph isolates, especially their traits allowing them adapt elevated growth temperatures. The genetic signatures Methylocaldum species have been detected many terrestrial aquatic ecosystems. A small set representatives this genus has isolated maintained culture. is commonly described as...
Abstract Phytoplankton is responsible for most primary production in Antarctica, but the short timescale dynamics of its size structure and composition are poorly described understood. The abundance phytoplankton Fildes Bay, western Antarctic Peninsula, was followed 12 days during summer using a range methods, including fractionation chlorophyll, microscopy, flow cytometry terminal-restriction fragment length polymorphism (T-RFLP) plastid 16S rRNA gene. A rapid increase biomass cell occurred...
The largest known bacteria, Thiomargarita spp., have yet to be isolated in pure culture, but their large size allows for individual cells monitored time course experiments or individually sorted omics-based investigations. Here we investigated the metabolism of spp. by using a novel application tetrazolium-based dye that measures oxidoreductase activity. When coupled with microscopy, staining tetrazolium-formazan metabolic responses tracked absence observable cell division. Additionally,...
Here, we report the draft genome sequences of strains HS012 and HS039, which were isolated from cnidarian polyps that had recently undergone metamorphosis. Genomic analyses place these within Phaeobacter Leisingera genera, members Roseobacter group.
Abstract Until recently, the cellular envelopes of bacteria were regarded as static and rigid relative to those eukaryotes. While investigating peptidoglycan synthesis in populations giant sulfur bacteria, Candidatus Thiomargarita spp., we observed internal vesicle-like features (VLFs). VLFs, imaged following active incorporation D-amino acids, appear begin invaginations delaminations envelope. Staining with wheat germ agglutinin confirmed presence while polymyxin B revealed that outer...
ABSTRACT The largest known bacteria, Thiomargarita spp., have yet to be isolated in pure culture, but their large size allows for individual cells followed time course experiments, or individually sorted ‘omics-based investigations. Here we report a novel application of tetrazolium-based dye that measures the flux reductase production from catabolic pathways investigate metabolic activity spp. When coupled microscopy, staining with tetrazolium-formazan responses tracked absence observable...