A5017447077- Congenital heart defects research
- Congenital Heart Disease Studies
- Cardiac Valve Diseases and Treatments
- Metabolism and Genetic Disorders
- Cardiac Arrhythmias and Treatments
- Cardiomyopathy and Myosin Studies
- Connective tissue disorders research
- Tissue Engineering and Regenerative Medicine
- Coronary Artery Anomalies
- Adrenal Hormones and Disorders
- Cell Adhesion Molecules Research
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
- Dermatological and Skeletal Disorders
- Liver Disease Diagnosis and Treatment
- Cardiac electrophysiology and arrhythmias
- Biochemical and Molecular Research
- Cardiovascular Effects of Exercise
- Muscle Physiology and Disorders
- Peptidase Inhibition and Analysis
- Connexins and lens biology
- Genetic Neurodegenerative Diseases
- Diabetes and associated disorders
- Renal function and acid-base balance
- Erythrocyte Function and Pathophysiology
Medical University of South Carolina
2016-2025
University of Missouri
2023
Queen Nandi Regional Hospital
2020
University of KwaZulu-Natal
2020
Amsterdam UMC Location University of Amsterdam
1995-2013
Newcastle University
2013
New York University
2008
Icahn School of Medicine at Mount Sinai
2008
National Institutes of Health
2008
University of Pennsylvania
2000-2008
Background Some of the problems concerning origin inlet component definitive right ventricle were resolved in a previous study which we showed it to be derived exclusively from embryonic ventricle. Questions remain, however, relative contributions endocardial cushion tissue and myocardium valvar apparatus guarding atrioventricular orifice leaflets. Methods Results The formation tricuspid valve was studied by scanning electron microscopic immunohistochemical techniques. Concurrent with...
ABSTRACT Pax3 encodes a transcription factor expressed during mid-gestation in the region of dorsal neural tube that gives rise to migrating crest populations. In absence Pax3, both humans and mice develop with defects. Homozygous Splotch embryos lack die by embryonic day 13.5 cardiac defects resemble those induced ablation chick models. This has led hypothesis is required for migration. However, derivatives Pax3-expressing precursor cells have not been previously defined, within heart well...
ABSTRACT Transgenic mice were generated containing a cytomegaloviral promoter driven construct (CMV43) expressing the gap junction polylpeptide connexin 43. RNA and protein analysis confirmed that transgene was being expressed. In situ hybridization of embryo sections revealed expression targeted to dorsal neural tube in subpopulations crest cells. This pattern identical seen transgenic harboring other constructs by (Kothary, R., Barton, S. C., Franz, T., Norris, M. L., Hettle, Surani, A. H....
Abstract The histogenesis of the separation between atrial and ventricular myocardium at atrioventricular junction in developing human heart has been investigated immunohistochemically by using monoclonal antibodies specific for cushion tissue, mesenchymal cells, myocardium, primary ring. It was found that insulation muscle masses atrium ventricle is established fusion tissues sulcus (located epicardial side junctional myocardium) with those cushions endocardial myocardium). This process...
Abstract A monoclonal antibody raised against an extract from the G anglion N odosum of chick and designated GIN2 proves to bind specifically a subpopulation cardiomyocytes in embryonic human heart. In youngest stage examined (Carnegie 14, i. e., 4 1/2 weeks development) these GIN2‐expressing cells are localized myocardium that surrounds foramen between left right ventricle. lesser curvature cardiac loop this “primary” ring occupies lower part wall atrioventricular canal. During subsequent...
The His-Purkinje system (HPS) is a network of conduction cells responsible for coordinating the contraction ventricles. Earlier studies using bipolar electrodes indicated that functional maturation HPS in chick embryo marked by topological shift sequence activation ventricle. Namely, at around completion septation, an immature base-to-apex ventricular was reported to convert apex-to-base pattern characteristic mature heart. Previously, we have proposed hemodynamics and/or mechanical...
Mitral valve prolapse (MVP) affects 1 in 40 people and is the most common indication for mitral surgery. MVP can cause arrhythmias, heart failure, sudden cardiac death, to date, causes of this disease are poorly understood. We now demonstrate that defects primary cilia genes their regulated pathways familial sporadic nonsyndromic cases. Our expression studies genetic ablation experiments confirmed a role regulating ECM deposition during development. Loss development resulted progressive...
Abstract —The minK gene encodes a 129-amino acid peptide the expression of which modulates function cardiac delayed rectifier currents ( I Kr and Ks ), mutations in are now recognized as one cause congenital long-QT syndrome. We have generated minK- deficient mice bacterial lacZ has been substituted for coding region such that β-galactosidase is controlled by endogenous regulatory elements. In myocytes isolated from wild-type neonatal mice, rarely recorded, while common. (–/–) myocytes,...
Connexin 43 (Cx43) knockout mice and transgenic (CMV43) overexpressing the Cx43 gap junction gene exhibit heart defects involving conotruncus right ventricle. Based on phenotype transgene expression pattern, we previously proposed that may reflect a role for junctions in modulation of cardiac neural crest development. To further elucidate mechanism by which these arise, fetal structure function were examined magnetic resonance microscopy Doppler echocardiography. Magnetic E14.5 fetuses...
BACKGROUND The mechanics involved in development of the inlet component morphologically right ventricle are, as yet, undecided. Some argue that this is derived from descending limb ventricular loop, and apical trabecular components muscular septum have separate developmental origins. Others state entirety grows ascending septum, apart its outer component, has a unitary origin. We now material human embryos at our disposal, which, we believe, solves conundrum. METHODS AND RESULTS used...