A5089127172- Congenital heart defects research
- Congenital Heart Disease Studies
- Cardiomyopathy and Myosin Studies
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Cardiac Structural Anomalies and Repair
- Coronary Artery Anomalies
- Cardiac Valve Diseases and Treatments
- Physiological and biochemical adaptations
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Conditions and Treatments
- Cardiac Arrhythmias and Treatments
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Cardiac Fibrosis and Remodeling
- Cancer, Hypoxia, and Metabolism
- Nitric Oxide and Endothelin Effects
- Connexins and lens biology
- Birth, Development, and Health
- RNA modifications and cancer
- Cardiac Imaging and Diagnostics
- Marine animal studies overview
- Cardiac Ischemia and Reperfusion
- Photoreceptor and optogenetics research
- Hormonal Regulation and Hypertension
Charles University
2016-2025
Czech Academy of Sciences
2016-2025
Czech Academy of Sciences, Institute of Physiology
2016-2025
King's College London
2016
Centro Cardiologico Monzino
2016
Institute of Experimental Cardiology
2016
Imperial College London
2016
University of Florence
2016
Manchester Metropolitan University
2016
Medical University of South Carolina
2000-2012
The combination of optical clarity and large scale mutants makes the zebrafish vital for developmental biologists. However, there is no comprehensive reference morphology function this animal. Since study gene expression must be integrated with structure function, we undertook a longitudinal to define cardiac physiology developing zebrafish. Our studies included 48-hr, 5-day, 2-week, 4-week, 3-month post-fertilization We measured ventricular body wet weights, performed morphologic analysis...
Adult myocardium adapts to changing functional demands by hyper- or hypotrophy while the developing heart reacts hypoplasia. How embryonic myocardial architecture adjusts experimentally altered loading is not known. We subjected chick hearts mechanically study its influence upon ventricular myoarchitecture. Chick were conotruncal banding (increased afterload model), left atrial ligation clipping, creating a combined model of increased preload in right ventricle and decreased ventricle....
Zebrafish and Xenopus have become popular model organisms for studying vertebrate development of many organ systems, including the heart. However, it is not clear whether single ventricular hearts these species possess any equivalent specialized conduction system found in higher vertebrates. Isolated adult zebrafish ( Danio rerio) African toads laevis) were stained with voltage-sensitive dye optically mapped spontaneous paced rhythms followed by histological examination focusing on...
The His-Purkinje system (HPS) is a network of conduction cells responsible for coordinating the contraction ventricles. Earlier studies using bipolar electrodes indicated that functional maturation HPS in chick embryo marked by topological shift sequence activation ventricle. Namely, at around completion septation, an immature base-to-apex ventricular was reported to convert apex-to-base pattern characteristic mature heart. Previously, we have proposed hemodynamics and/or mechanical...
Noncompaction cardiomyopathy is characterized by the presence of extensive trabeculations, which could lead to heart failure and malignant arrhythmias. How trabeculations resolve form compact myocardium poorly understood. Elucidation this process critical understanding pathophysiology noncompaction disease. Here we use genetic lineage tracing mark Nppa+ or Hey2+ cardiomyocytes as trabecular components ventricular wall. We find that cardiomyocytes, respectively, from endocardial epicardial...
Endocardial cushions are critical to maintain unidirectional blood flow under constantly increasing hemodynamic forces, but the interrelationship between endocardial cushion structure and mechanics of atrioventricular junction function is poorly understood. Atrioventricular (AV) canal motions velocities embryonic chicks at Hamburger Hamilton (HH) stages 17, 21, 25 were quantified using ultrasonography. Similar zebrafish heart, HH17 AV segment functions like a suction pump, with expanding in...
Hemodynamics influence cardiac development, and alterations in blood flow may lead to impaired growth malformations. The developing myocardium adapts augmented workload by increasing cell number (hyperplasia). aim of this study was determine the ventricular preload on fetal myocyte proliferation manipulation intracardiac shunting at atrial level. We hypothesized that partial clipping right appendage would increase left ventricle and, turn, an chamber volume myocardial mass based...
The molecular mechanisms regulating sympathetic innervation of the heart during embryogenesis and its importance for cardiac development function remain to be fully elucidated. We generated mice in which conditional knockout (CKO) Hif1a gene encoding transcription factor hypoxia-inducible 1α (HIF-1α) is mediated by an Islet1-Cre transgene expressed outflow tract, right ventricle atrium, pharyngeal mesoderm, peripheral neurons, hindlimbs. These Hif1aCKO demonstrate significantly decreased...
Abstract Patterns of DNA synthesis in the developing mouse heart between ED7.5–18.5 were studied by a combination thymidine and bromodeoxyuridine labeling techniques. From earliest stages, we found zones slow myocyte proliferation at both venous arterial poles heart, as well atrioventricular region. The index was distinctly higher nonmyocardial populations (endocardium, epicardium, cardiac cushions). Ventricular trabeculae showed lower proliferative activity than ventricular compact layer...
Abstract Hypoplastic left heart syndrome (HLHS) is a rare but deadly congenital malformation, which can be created experimentally in the chick embryo by atrial ligation (LAL). The goal of this study was to examine cellular changes leading profound remodeling ventricular myocardial architecture that occurs model. Hypoplasia structures produced after 3H‐thymidine prelabeling partial LAL at stage 24, thereby reducing its volume, and redistributing blood preferentially developing right ventricle...
Impulse-conducting Purkinje fibers differentiate from myocytes during embryogenesis. The conversion of contractile into conduction cells is induced by the stretch/pressure-induced factor, endothelin (ET). Active ET produced via proteolytic processing its precursor ET-converting enzyme 1 (ECE1) and triggers signaling binding to receptors. In embryonic chick heart, receptors are expressed all myocytes, but ECE1 predominantly in endothelial coronary arteries endocardium along which fiber...
Abstract The chick embryo has long been a favorite model system for morphologic and physiologic studies of the developing heart, largely because its easy visualization amenability to experimental manipulations. However, this advantage is diminished after 5 days incubation, when rapidly growing chorioallantoic membranes reduce visibility embryo. Using high‐frequency ultrasound, we show that embryonic cardiovascular structures can be readily visualized throughout period Stages 9–39. At most...
The sinus venous myocardium, comprising the sinoatrial node (SAN) and horns (SH), is a region subject to congenital malformations cardiac arrhythmias. It differentiates from symmetric bilateral mesenchymal precursors, but morphological, molecular, functional left/right differences are progressively established through development. role of laterality gene Pitx2 in this process unknown. We aimed elucidate molecular events driving patterning venosus (SV) myocardium by using myocardial knockout...
Background:The aim of our study was to investigate the relationship between cardiac magnetic resonance (CMR)-derived native T1 relaxation time and myocardial extracellular volume (ECV) fraction extent diffuse fibrosis (DMF) on targeted left ventricular (LV) biopsy.Methods Results:The population consisted 40 patients (age 63±8 years, 65% male) undergoing valve and/or ascending aorta surgery for severe aortic stenosis (77.5%), root dilatation (7.5%) or regurgitation (15%). The assessed in...