A5087383028- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Pancreatic function and diabetes
- Cardiac Fibrosis and Remodeling
- Liver physiology and pathology
- Neonatal Respiratory Health Research
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
- Galectins and Cancer Biology
- Gene expression and cancer classification
- Cancer Cells and Metastasis
- Apelin-related biomedical research
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Cardiomyopathy and Myosin Studies
- Congenital Heart Disease Studies
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Cardiac Structural Anomalies and Repair
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Cardiovascular Function and Risk Factors
Westlake University
2022-2025
Anqing City Hospital
2023
Chinese Academy of Sciences
2011-2022
Center for Excellence in Molecular Cell Science
2017-2022
University of Chinese Academy of Sciences
2016-2022
Zhejiang University
2012-2022
First Affiliated Hospital Zhejiang University
2020-2021
Shanghai Institutes for Biological Sciences
2011-2020
International Peace Maternity & Child Health Hospital
2018-2019
Shanghai Jiao Tong University
2011-2019
The heart needs blood vessels, too For the newborn to grow quickly, heart's own vessels must as well. Researchers have assumed that preexisting fetal coronary expand cause this postnatal vascular growth. Instead, Tian et al. now show that, for most part, brandnew form within neonatal (see Perspective by Burns and Burns). This ability produce new after birth may one day help researchers work out how promote cardiovascular regeneration injury or disease. Science , issue p. 90 ; see also 28
Organ homeostasis is orchestrated by time- and spatially restricted cell proliferation. Studies identifying cells with superior proliferative capacities often rely on the lineage tracing of a subset populations, which introduces potential selective bias. In this work, we developed genetic system [proliferation tracer (ProTracer)] incorporating dual recombinases to seamlessly record proliferation events entire populations over time in multiple organs. mouse liver, ProTracer revealed more...
Whether the adult mammalian heart harbors cardiac stem cells for regeneration of cardiomyocytes is an important yet contentious topic in field cardiovascular regeneration. The putative myocyte cell populations recognized without specific markers, such as cardiosphere-derived cells, or with markers Sca1+, Bmi1+, Isl1+, Abcg2+ have been reported. Moreover, it remains unclear whether unknown unidentified exist and give rise to de novo heart.To address this question relying on a particular...
The mechanisms that promote the generation of new coronary vasculature during cardiac homeostasis and after injury remain a fundamental clinically important area study in cardiovascular field. Recently, it was reported mesenchymal-to-endothelial transition (MEndoT) contributes to substantial numbers endothelial cells myocardial infarction. Therefore, MEndoT has been proposed as paradigm mediating neovascularization is considered promising therapeutic target regeneration. Here, we show...
Background: Mutations in low-density lipoprotein (LDL) receptor ( LDLR ) are one of the main causes familial hypercholesterolemia, which induces atherosclerosis and has a high lifetime risk cardiovascular disease. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is an effective tool for gene editing to correct mutations thus ameliorate Methods: goal this work was determine whether vivo somatic cell through CRISPR/Cas9 delivered by adeno-associated virus...
Abstract Following severe liver injury, when hepatocyte-mediated regeneration is impaired, biliary epithelial cells (BECs) can transdifferentiate into functional hepatocytes. However, the subset of BECs with such facultative tissue stem cell potential, as well mechanisms enabling transdifferentiation, remains elusive. Here we identify a transitional progenitor (TLPC), which originates from and differentiates hepatocytes during injury. By applying dual genetic lineage tracing approach,...
Coronary arteries bring blood flow to the heart muscle. Understanding developmental program of coronary provides insights into treatment artery diseases. Multiple sources have been described as contributing including proepicardium, sinus venosus (SV), and endocardium. However, origins vessels are still under intense study. We produced a new genetic tool for studying development, an AplnCreER mouse line, which expresses inducible Cre recombinase specifically in developing vessels....
Cardiac malformations due to aberrant development of the atrioventricular (AV) valves are among most common forms congenital heart diseases. Normally, valve mesenchyme is formed from an endothelial mesenchymal transition (EMT) cells endocardial cushions. Yes-associated protein 1 (YAP1) has been reported regulate EMT in vitro, addition its known role as a major regulator organ size and cell proliferation vertebrates, leading us hypothesize that YAP1 required for development. We tested this...
Rationale: There is persistent uncertainty regarding the developmental origins of coronary vessels, with 2 principal sources suggested as ventricular endocardium or sinus venosus (SV). These proposed implicate fundamentally distinct mechanisms vessel formation. Resolution this controversy critical for deciphering programs that result in formation vessels and has implications research on therapeutic angiogenesis. Objective: To resolve over origin vessels. Methods Results: We first generated...
Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem (CSCs), are in clinical trials to investigate their ability stimulate regeneration and repair. These studies were initially motivated the purported cardiogenic activity of these cells. Recent lineage tracing using Kit promoter drive expression inducible Cre recombinase showed that CSCs had highly limited activity, inadequate support efficient Here we reassess data investigating identity immediately after labeling. Our instant approach...
Myocardial infarction (MI) is one of the leading causes morbidity and mortality world-wide. Whether endogenous repair regenerative ability could be augmented by drug administration an important issue for generation novel therapeutic approach. Recently it was reported that in mice pretreated with thymosin beta 4 (TB4) subsequently subjected to experimental MI, a subset epicardial cells differentiated into cardiomyocytes. In clinical settings, priming TB4 prior MI impractical. Here we tested...
Abstract Under pathophysiological conditions in adults, endothelial cells (ECs) sprout from pre-existing blood vessels to form new ones by a process termed angiogenesis. During embryonic development, Apelin (APLN) is robustly expressed vascular ECs. In adult mice, however, APLN expression the vasculature significantly reduced. Here we show that reactivated ECs after ischaemia insults. models of both injury and tumor angiogenesis, find Apln-CreER genetically labels sprouting but not quiescent...
Ischemic heart disease is the leading cause of death worldwide. Myocardial infarction results in an irreversible loss cardiomyocytes with subsequent adverse remodeling and failure. Identifying new sources for promoting their formation represents a goal cardiac biology regenerative medicine. Within past decade, many types putative stem cells (CSCs) have been reported to regenerate injured myocardium by differentiating into cardiomyocytes. Some these CSCs translated from bench bed therapeutic...
Hepatocytes are functionally heterogeneous and divided into two distinct populations based on their metabolic zonation: the periportal pericentral hepatocytes. During liver injury regeneration, cellular dynamics of these remain largely elusive. Here we show that major facilitator super family domain containing 2a (Mfsd2a), previously known to maintain blood-brain barrier function, is a zonation marker. By genetic lineage tracing Mfsd2a+ hepatocytes, population decreases during homeostasis....
Although the mammalian heart can regenerate during neonatal stage, this endogenous regenerative capacity is lost with age. Importantly, replication of cardiomyocytes has been found to be key mechanism responsible for cardiac regeneration. Unraveling transcriptional regulatory network inducing cardiomyocyte will, therefore, crucial development novel therapies drive repair after injury. Here, we investigated whether transcription factor GATA4 required mouse Using cryoinjury and apical...
Noncompaction cardiomyopathy is characterized by the presence of extensive trabeculations, which could lead to heart failure and malignant arrhythmias. How trabeculations resolve form compact myocardium poorly understood. Elucidation this process critical understanding pathophysiology noncompaction disease. Here we use genetic lineage tracing mark Nppa+ or Hey2+ cardiomyocytes as trabecular components ventricular wall. We find that cardiomyocytes, respectively, from endocardial epicardial...
Elucidation of the role different cell lineages in liver could offer avenues to drive regeneration. Previous studies showed that SOX9+ hepatocytes can differentiate into ductal cells after injuries. It is unclear whether are uni- or bipotent progenitors at a single-cell level during injury. Here, we developed genetic tracing system delineate lineage potential homeostasis and Fate-mapping data these respond specifically injuries, with some contributing substantial number cells. Clonal...