A5069123275- Neonatal Respiratory Health Research
- Congenital heart defects research
- Tracheal and airway disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Congenital Diaphragmatic Hernia Studies
- Diabetes and associated disorders
- Cardiac Fibrosis and Remodeling
- RNA modifications and cancer
- Liver physiology and pathology
- Cardiac and Coronary Surgery Techniques
- Tissue Engineering and Regenerative Medicine
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Virus-based gene therapy research
- Wnt/β-catenin signaling in development and cancer
- Organ Transplantation Techniques and Outcomes
- Cancer Genomics and Diagnostics
- Congenital Heart Disease Studies
University of Chinese Academy of Sciences
2019-2025
Center for Excellence in Molecular Cell Science
2019-2025
Chinese Academy of Sciences
2019-2023
Shanghai Institutes for Biological Sciences
2019-2020
Abstract Following severe liver injury, when hepatocyte-mediated regeneration is impaired, biliary epithelial cells (BECs) can transdifferentiate into functional hepatocytes. However, the subset of BECs with such facultative tissue stem cell potential, as well mechanisms enabling transdifferentiation, remains elusive. Here we identify a transitional progenitor (TLPC), which originates from and differentiates hepatocytes during injury. By applying dual genetic lineage tracing approach,...
Alveolar type 2 (AT2) cells are stem of the alveolar epithelia. Previous genetic lineage tracing studies reported multiple cellular origins for AT2 after injury. However, conventional based on Cre-loxP has limitation non-specific labeling. Here, we introduced a dual recombinase-mediated intersectional approach, enabling precise investigation during lung homeostasis, injury, and repair. We found AT1 cells, being terminally differentiated, did not contribute to injury Distinctive yet...
Lung injury activates epithelial stem or progenitor cells for alveolar repair and regeneration. Unraveling the origin fate of injury-induced progenitors is crucial elucidating lung mechanisms. Here, we report that p63-expressing emerge upon bleomycin-induced mouse injury. Single-cell RNA sequencing clonal analysis reveal these p63+ proliferate rapidly differentiate into type 1 2 through different trajectories. Dual recombinase-mediated sequential genetic-lineage tracing demonstrates...
Unraveling cell fate plasticity during tissue homeostasis and repair can reveal actionable insights for stem biology regenerative medicine. In the pancreas, it remains controversial whether lineage transdifferentiation among exocrine cells occur under pathophysiological conditions. Here, to address this question, we used a dual recombinase-mediated genetic system that enables simultaneous tracing of pancreatic acinar ductal using two distinct reporters, avoiding "ectopic" labeling by...
The developing heart is composed of cardiomyocytes and noncardiomyocytes since the early stage. It generally believed that including cardiac progenitors contribute to new looping heart. However, it remains unclear what cellular dynamics nonmyocyte cardiomyocyte conversion are when lineage segregation occurs during development. also unknown whether contributes neonatal regeneration.We quantify in embryonic hearts determine 2 cell lineages segregate Moreover, we directly test if generated a...
Genetic lineage tracing is widely used to study organ development and tissue regeneration. Multicolor reporters are a powerful platform for simultaneously tracking discrete cell populations. Here, combining Dre-rox Cre-loxP systems, we generated new dual-recombinase reporter system, called Rosa26 traffic light ( R26-TLR ), monitor red, green, yellow fluorescence. Using this system with the three distinct fluorescent combined on one allele, found that readouts of two recombinases Cre Dre...
Abstract The adult pancreatic ducts have long been proposed to contain rare progenitors, some of which expressing Ngn3, that generate new beta cells in endocrine-islet homeostasis. Due their postulated rarity and the lack definitive markers, existence or absence ductal endocrine progenitors remains unsettled despite many studies. Genetic lineage tracing Ngn3 + with currently available CreER drivers has complicated by off-target labeling pre-existing cells. Here, using...
Genetic lineage tracing is widely used to study organ development and tissue regeneration. Multicolor reporters are a powerful platform for simultaneously tracking discrete cell populations. Here, combining Dre-rox Cre-loxP systems, we generated new dual-recombinase reporter system, called Rosa26 traffic light (R26-TLR), monitor red, green, yellow fluorescence. Using this system with the three distinct fluorescent combined on one allele, found that readouts of two recombinases Cre Dre...
Cellular proliferation is a basic process during organ development, tissue homeostasis, and/or disease progression. Likewise, after injury typically multiple cell lineages respond to various cues and proliferate initiate repair remodeling of the injured tissue. Unravelling specific role one type its lineage in context whole organism regeneration progression would provide valuable information on these processes. Here we reported new genetic system inhibit tissue-specific manner. We generated...
Abstract Lung injury activates epithelial stem or progenitor cells for alveolar repair and regeneration. However, the origin fate of injury-induced progenitors are poorly defined. Here, we report that p63-expressing emerge upon bleomycin-induced lung injury. These p63 + proliferate rapidly differentiate into type 1 (AT1) 2 (AT2) through distinct trajectories. Dual recombinase-mediated sequential genetic lineage tracing reveals originate from airway secretory subsequently generate cells....