A5100398316- Congenital heart defects research
- Neonatal Respiratory Health Research
- Hormonal Regulation and Hypertension
- Sodium Intake and Health
- Air Quality and Health Impacts
- Nutritional Studies and Diet
- Blood Pressure and Hypertension Studies
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Circular RNAs in diseases
- Pluripotent Stem Cells Research
- Congenital Diaphragmatic Hernia Studies
- Cardiac Fibrosis and Remodeling
- Climate Change and Health Impacts
- Tracheal and airway disorders
- Health disparities and outcomes
- Liver physiology and pathology
- Renin-Angiotensin System Studies
- Pancreatic function and diabetes
- Diabetes, Cardiovascular Risks, and Lipoproteins
Capital Medical University
2016-2025
University of Chinese Academy of Sciences
2017-2025
Chinese Academy of Sciences
2016-2025
Center for Excellence in Molecular Cell Science
2017-2025
Fujian Institute of Research on the Structure of Matter
2023-2025
State Key Laboratory of Structural Chemistry
2025
Shandong Provincial QianFoShan Hospital
2017-2024
Shandong First Medical University
2022-2024
University of Benin
2024
Xiangyang Central Hospital
2024
Organ homeostasis is orchestrated by time- and spatially restricted cell proliferation. Studies identifying cells with superior proliferative capacities often rely on the lineage tracing of a subset populations, which introduces potential selective bias. In this work, we developed genetic system [proliferation tracer (ProTracer)] incorporating dual recombinases to seamlessly record proliferation events entire populations over time in multiple organs. mouse liver, ProTracer revealed more...
Whether the adult mammalian heart harbors cardiac stem cells for regeneration of cardiomyocytes is an important yet contentious topic in field cardiovascular regeneration. The putative myocyte cell populations recognized without specific markers, such as cardiosphere-derived cells, or with markers Sca1+, Bmi1+, Isl1+, Abcg2+ have been reported. Moreover, it remains unclear whether unknown unidentified exist and give rise to de novo heart.To address this question relying on a particular...
Abstract Following severe liver injury, when hepatocyte-mediated regeneration is impaired, biliary epithelial cells (BECs) can transdifferentiate into functional hepatocytes. However, the subset of BECs with such facultative tissue stem cell potential, as well mechanisms enabling transdifferentiation, remains elusive. Here we identify a transitional progenitor (TLPC), which originates from and differentiates hepatocytes during injury. By applying dual genetic lineage tracing approach,...
Alveolar type 2 (AT2) cells are stem of the alveolar epithelia. Previous genetic lineage tracing studies reported multiple cellular origins for AT2 after injury. However, conventional based on Cre-loxP has limitation non-specific labeling. Here, we introduced a dual recombinase-mediated intersectional approach, enabling precise investigation during lung homeostasis, injury, and repair. We found AT1 cells, being terminally differentiated, did not contribute to injury Distinctive yet...
Hepatocytes are functionally heterogeneous and divided into two distinct populations based on their metabolic zonation: the periportal pericentral hepatocytes. During liver injury regeneration, cellular dynamics of these remain largely elusive. Here we show that major facilitator super family domain containing 2a (Mfsd2a), previously known to maintain blood-brain barrier function, is a zonation marker. By genetic lineage tracing Mfsd2a+ hepatocytes, population decreases during homeostasis....
Monitoring of cell-cell communication in multicellular organisms is fundamental to understanding diverse biological processes such as embryogenesis and tumorigenesis. To track contacts vivo, we developed an intercellular genetic technology monitor contact trace cell histories by permanently marking between cells. In mice, engineered artificial Notch ligand into one (the sender cell) receptor another receiver cell). Contact the cells triggered a synthetic signaling that activated downstream...
BACKGROUND: The developmental diversity among smooth muscle cells (SMCs) plays a crucial role in segment-specific aortic diseases. However, traditional genetic approaches are inadequate for enabling vivo analysis of disease susceptibility associated with cellular origin. There is an urgent need to build technologies that target different origins investigate the mechanisms aortopathies, thereby facilitating development effective therapeutics. METHODS: To address this challenge, we developed...
Abstract Cardiac regeneration involves the generation of new cardiomyocytes from cycling cardiomyocytes. Understanding cell-cycle activity pre-existing provides valuable information to heart repair and regeneration. However, anatomical locations in situ dynamics remain unclear. Here we develop a genetic approach for temporally seamless recording cardiomyocyte-specific vivo. We find that majority are positioned subendocardial muscle left ventricle, especially papillary muscles. Clonal...
Background: Cardiac fibrosis is a lethal outcome of excessive formation myofibroblasts that are scar-forming cells accumulated after heart injury. It has been reported cardiac endothelial (ECs) contribute to substantial portion through mesenchymal transition (EndoMT). Recent lineage tracing studies demonstrate derived from the expansion resident fibroblasts rather than transdifferentiation ECs. However, it remains unknown whether ECs can transdifferentiate into reversibly or EndoMT genes...
Tissue-resident macrophages play essential functions in the maintenance of tissue homeostasis and repair. Recently, endocardium has been reported as a de novo hemogenic site for contribution hematopoietic cells, including cardiac macrophages, during embryogenesis. These observations challenge current consensus that hematopoiesis originates from endothelium within yolk sac dorsal aorta. Whether developing such potential requires further investigation. Here, we generated new genetic tools to...
Lung injury activates epithelial stem or progenitor cells for alveolar repair and regeneration. Unraveling the origin fate of injury-induced progenitors is crucial elucidating lung mechanisms. Here, we report that p63-expressing emerge upon bleomycin-induced mouse injury. Single-cell RNA sequencing clonal analysis reveal these p63+ proliferate rapidly differentiate into type 1 2 through different trajectories. Dual recombinase-mediated sequential genetic-lineage tracing demonstrates...
Abstract Background Chinese menopausal women comprise a large population and the in it experience symptoms many different ways. Their health related quality of life (HRQOL) is not particularly well studied. Our study intends to evaluate influence menopause on HRQOL explore other risk factors for rural China. Methods An interview was conducted from June August 2010 Beijing based cross-sectional design. 1,351 aged 40–59 were included study. measured using EuroQol Group’s 5-domain (EQ5D)...
Heart valve disease is a major clinical problem worldwide. Cardiac development and homeostasis need to be precisely controlled. Hippo signaling essential for organ tissue homeostasis, while its role in formation morphology maintenance remains unknown. VGLL4 transcription cofactor vertebrates we found it was mainly expressed interstitial cells at the post-EMT stage maintained till adult stage. Tissue specific knockout of different cell lineages revealed that only loss endothelial lineage led...
In vivo genomic engineering is instrumental for studying developmental biology and regenerative medicine. Development of novel systems with more site-specific recombinases (SSRs) that complement the commonly used Cre-loxP would be valuable precise lineage tracing genome editing. Here, we introduce a new SSR system via Nigri-nox. By generating tissue-specific Nigri knock-in its responding nox reporter mice, show Nigri-nox works efficiently in by targeting specific tissues. As orthogonal to...
Abstract During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of peritoneum pericardium invade deeply respective organs promote repair. Here we report a dual recombinase-mediated genetic system designed trace in vivo by intersectional detection two characteristic markers. Lineage tracing this method shows accumulation during lung liver injury on surface visceral...