A5025618652- Sulfur Compounds in Biology
- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Renin-Angiotensin System Studies
- Eicosanoids and Hypertension Pharmacology
- Cardiac electrophysiology and arrhythmias
- Cardiac Ischemia and Reperfusion
- Cardiac Fibrosis and Remodeling
- Cardiomyopathy and Myosin Studies
- RNA modifications and cancer
- Folate and B Vitamins Research
- Nuclear Receptors and Signaling
- Congenital heart defects research
- Immune cells in cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Atherosclerosis and Cardiovascular Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Mast cells and histamine
- Neuroscience and Neuropharmacology Research
- Animal testing and alternatives
- IL-33, ST2, and ILC Pathways
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Inflammatory mediators and NSAID effects
- Biochemical effects in animals
Nanjing Medical University
2016-2025
Wuxi People's Hospital
2018-2025
Harbin Medical University
2010-2025
First People’s Hospital of Zunyi
2025
Suzhou Municipal Hospital
2021-2025
Ministry of Education of the People's Republic of China
2023-2025
Ningbo University
2025
Inner Mongolia Agricultural University
2025
Monash University
2024
Dongfang Electric Corporation (China)
2024
Reproducibility is a current concern for everyone involved in the conduct and publication of biomedical research. Recent attempts testing reproducibility, particularly reproducibility project cancer biology published elife (https://elifesciences.org/collections/9b1e83d1/reproducibility-project-cancer-biology), have exposed major difficulties repeating preclinical experimental work. It thought that some these relate to uncertainty about provenance tools, lack clarity methodology use...
Hydrogen sulfide (H2S) has been shown to have powerful antioxidative and anti-inflammatory properties that can regulate multiple cardiovascular functions. However, its precise role in diabetes-accelerated atherosclerosis remains unclear. We report here H2S reduced aortic atherosclerotic plaque formation with reduction superoxide (O2 (-)) generation the adhesion molecules streptozotocin (STZ)-induced LDLr(-/-) mice but not LDLr(-/-)Nrf2(-/-) mice. In vitro, inhibited foam cell formation,...
Rationale: Endothelial injury is an initial mechanism mediating cardiovascular disease. Objective: Here, we investigated the effect of hyperhomocysteinemia on programed cell death in endothelial cells (EC). Methods and Results: We established a novel flow-cytometric gating method to define pyrotosis (Annexin V − /Propidium iodide + ). In cultured human EC, found that: (1) homocysteine lipopolysaccharide individually synergistically induced inflammatory pyroptotic noninflammatory apoptotic...
Natural products continue to be an important source of medicines and drug templates. Indeed, it has been estimated that approximately a third all Food Drug Administration (FDA)-approved drugs over the past 20 years are based on natural or their derivatives (Thomford et al., 2018). This is likely due vast chemical diversity products, which increases probability finding structurally distinct 'lead compounds' for different targets diseases (Gu 2013). For instance, Dictionary Products thus far...
Organ homeostasis is orchestrated by time- and spatially restricted cell proliferation. Studies identifying cells with superior proliferative capacities often rely on the lineage tracing of a subset populations, which introduces potential selective bias. In this work, we developed genetic system [proliferation tracer (ProTracer)] incorporating dual recombinases to seamlessly record proliferation events entire populations over time in multiple organs. mouse liver, ProTracer revealed more...
Shift work is associated with increased risk of acute myocardial infarction (AMI) and worsened prognosis. However, the mechanisms linking shift prognosis in AMI remain unclear. This study sought to investigate impact on reperfusion injury, a major determinant clinical outcomes AMI. Study patient data were obtained from database EARLY-MYO-CMR (Early Assessment Myocardial Tissue Characteristics by CMR STEMI) registry, which was prospective, multicenter registry patients ST-segment elevation...
Aortic aneurysm and aortic dissection (AAD) are life-threatening vascular diseases, with endothelium being the primary target for AAD treatment. Protein S-sulfhydration is a newly discovered posttranslational modification whose role in has not yet been defined. This study aims to investigate whether protein regulates its underlying mechanism.Protein endothelial cells (ECs) during was detected hub genes regulating homeostasis of were identified. Clinical data patients healthy controls...
The sarco(endo)plasmic reticulum Ca<sup>2+</sup>-ATPase isoform 2 (SERCA2) gene encodes both SERCA2a, the cardiac sarcoplasmic Ca<sup>2+</sup> pump, and SERCA2b, which is expressed in all tissues. To gain a better understanding of physiological functions SERCA2, we used targeting to develop mouse promoter 5′ end were eliminated. Mating heterozygous mutant mice yielded wild-type offspring; homozygous mutants not observed. RNase protection, Western blotting, biochemical analysis heart samples...
Cardiac hypertrophy and heart failure are known to be associated with a reduction in Ca2+-ATPase pump levels of the sarcoplasmic reticulum (SR). To determine whether, what extent, alterations Ca2+ numbers can affect contraction relaxation parameters heart, we have overexpressed cardiac SR specifically mouse using alpha-myosin heavy chain promoter. Analysis 2 independent transgenic lines demonstrated that sarco(endo)plasmic isoform (SERCA2a) mRNA were increased 3.88+/-0. 4-fold...
Atherosclerosis is associated with reduced vascular hydrogen sulfide (H2 S) biosynthesis. GYY4137 a novel slow-releasing H2 S compound that may effectively mimic the time course of release in vivo. However, it not known whether affects atherosclerosis.RAW 264.7 cells and human blood monocyte-derived macrophages were incubated oxidized low density lipoprotein (ox-LDL) with/without GYY4137. ApoE(-/-) mice fed high-fat diet for 4 weeks administered 30 days. Lipid atherosclerotic lesions...
Hydrogen sulfide (H2 S) is a gaseous signal molecule with antioxidative properties. Sirtuin 3 (SIRT3) closely associated mitochondrial function and oxidative stress. The study was to investigate whether how H2 S improved myocardial hypertrophy via SIRT3-dependent manner.Neonatal rat cardiomyocytes were pretreated NaHS (50 μM) for 4 h followed by angiotensin II (Ang II, 100 nM) 24 h. SIRT3 silenced siRNA technology. promoter activity expression, cell surface, hypertrophic gene mRNA oxygen...
Oxidative stress is a key contributor to endothelial dysfunction and associated cardiovascular pathogenesis. Hydrogen sulfide (H2S) an antioxidant gasotransmitter that protects cells against oxidative stress. Sirtuin3 (SIRT3), which belongs the silent information regulator 2 (SIR2) family, important deacetylase under H2S able regulate activity of several sirtuins. The present study aims investigate role SIRT3 in effect cells.Cultured EA.hy926 were exposed hydrogen peroxide (H2O2) as model...
Elucidation of the role different cell lineages in liver could offer avenues to drive regeneration. Previous studies showed that SOX9+ hepatocytes can differentiate into ductal cells after injuries. It is unclear whether are uni- or bipotent progenitors at a single-cell level during injury. Here, we developed genetic tracing system delineate lineage potential homeostasis and Fate-mapping data these respond specifically injuries, with some contributing substantial number cells. Clonal...
Background and Aims Protein S‐sulfhydration mediated by H 2 S has been shown to play important roles in several diseases. However, its precise role liver disease the related mechanism remain unclear. Approach Results We showed that streptozotocin (STZ)–treated high‐fat diet (HFD)–treated low‐density lipoprotein receptor–negative (LDLr −/− ) mice, donor GYY4137 ameliorated injury, decreased serum alanine aminotransferase (ALT) aspartate (AST) levels, mitigated lipid deposition, reduced...
Precision medicine is a buzz word mentioned frequently in the research world currently. Indeed, initiatives such as UK's 100,000 genomes project are based on premise that better understanding of an individual's biology and genomic profile enables clinicians to serve their patients. In some patients, this approach has led diagnosis rare disease effective treatment was not obvious without genetic information. light these technological advances, it seems unconscionable "sex," which probably...
S-nitrosylation (SNO), a prototypic redox-based posttranslational modification, is involved in the pathogenesis of cardiovascular disease. The aim this study was to determine role SNO MLP (muscle LIM protein) myocardial hypertrophy, as well mechanism by which SNO-MLP modulates hypertrophic growth response pressure overload.Myocardial samples from patients and animal models exhibiting hypertrophy were examined for level using biotin-switch methods. sites further identified through liquid...