A5004498470- Protease and Inhibitor Mechanisms
- Proteoglycans and glycosaminoglycans research
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Muscle Physiology and Disorders
- Peptidase Inhibition and Analysis
- Connective tissue disorders research
- Neuropeptides and Animal Physiology
- Influenza Virus Research Studies
- Cytokine Signaling Pathways and Interactions
- Glycosylation and Glycoproteins Research
- HER2/EGFR in Cancer Research
- Radiopharmaceutical Chemistry and Applications
- Immune Response and Inflammation
- Hydrological Forecasting Using AI
- Signaling Pathways in Disease
- vaccines and immunoinformatics approaches
- Congenital heart defects research
- Data Mining Algorithms and Applications
- Regulation of Appetite and Obesity
- Bone health and treatments
- Prostate Cancer Treatment and Research
- Exercise and Physiological Responses
- Blood Coagulation and Thrombosis Mechanisms
- Rough Sets and Fuzzy Logic
Deakin University
2012-2024
Cleveland Clinic Lerner College of Medicine
2009-2018
The University of Queensland
2017
Medical University of South Carolina
2011
University of Bristol
2007-2010
Cleveland Clinic
2008-2009
St Vincent's Hospital
2005
O'Brien Institute
2005
The University of Melbourne
2005
Queensland University of Technology
2000-2004
Abstract Purpose: The disintegrin metalloprotease ADAM-10 is a multidomain that potentially significant in tumor progression due to its extracellular matrix-degrading properties. Previously, mRNA was detected prostate cancer (PCa) cell lines; however, the presence of protein and cellular localization, regulation, role have yet be described. We hypothesized may regulated by growth factors such as 5α-dihydrotestosterone, insulin-like factor I, epidermal factor, known modulators PCa invasion....
The extracellular matrix (ECM) provides physical scaffolding for cellular constituents and initiates biochemical biomechanical cues that are required physiological activity of living tissues. ECM enzyme ADAMTS5, a member the ADAMTS (A Disintegrin-like Metalloproteinase with Thrombospondin-1 motifs) protein family, cleaves large proteoglycans such as aggrecan, leading to destruction cartilage osteoarthritis. However, its contribution viral pathogenesis immunity is currently undefined. Here,...
Heterotopic ossification (HO) is a significant clinical problem with incompletely resolved mechanisms. Here, the secreted metalloproteinases ADAMTS7 and ADAMTS12 are shown to comprise unique proteoglycan class that protects against tendency toward HO in mouse hindlimb tendons, menisci, ligaments. Adamts7 Adamts12 mRNAs were sparsely expressed murine forelimbs but strongly coexpressed skeletal muscle, ligaments, meniscal fibrocartilage. Adamts7–/– Adamts12–/– mice, not corresponding...
The A Disintegrin-like and Metalloproteinase domain with Thrombospondin-1 motifs (ADAMTS) enzymes comprise 19 mammalian zinc-dependent metalloproteinases (metzincins) homologues in a wide range of invertebrates. ADAMTS have broad functions development diseases due to their extracellular matrix remodelling activity. Here, we report detailed characterisation evolutionary conservation across vertebrates. Using bioinformatics complemented de novo sequencing, gene sequences for were obtained from...
The chondroitin sulfate proteoglycans (CSPGs) aggrecan, versican, and brevican are large aggregating extracellular matrix molecules that inhibit axonal growth of the mature central nervous system (CNS). ADAMTS proteoglycanases, including ADAMTS4 ADAMTS5, degrade CSPGs, representing potential targets for ameliorating growth-inhibition by CSPG accumulation after CNS injury. We investigated proteolysis CSPGs in mice homozygous Adamts4 or Adamts5 null alleles spinal cord injury (SCI)....
Ovarian cancer is a leading cause of mortality in women world-wide. Considerable progress has been made to characterize the different subtypes ovarian cancer, but specific therapies remain limited and prognosis poor. Cytokine signaling via interleukin-6 receptor (IL-6R) family related receptors implicated number cancers, including those with an origin. The leptin (LEPR) structurally these utilizes similar downstream pathways. LEPR diverse roles metabolism, appetite bone formation obesity...
Extracellular matrix remodeling has emerged as an important factor in many cancers. Proteoglycans, including versican (VCAN), are regulated via cleavage by the proteolytic actions of A Disintegrin-like And Metalloproteinase domain with Thrombospondin-1 motif (ADAMTS) family members. Alterations balance between Proteoglycans and ADAMTS enzymes have been proposed to contribute cancer progression. Here, we analyzed expression ADAMTS-15 human prostate cancer, investigated effects enforced cell...
Androgen-dependent prostate cancer (PrCa) xenograft models are required to study PrCa biology in the clinically relevant vivo environment.Human tissue from a femoral bone metastasis biopsy (BM18) was grown and passaged subcutaneously through male severe combined immune-deficient (SCID) mice. Human mitochondria (hMt), specific antigen (PSA), androgen receptor (AR), cytokeratin-18 (CK-18), pan-cytokeratin, high molecular weight-cytokeratin (HMW-CK) were assessed using immunohistochemistry...
Exogenous hyaluronan is known to alter muscle precursor cell proliferation, migration, and differentiation, ultimately inhibiting myogenesis in vitro. The aim of the current study was investigate role endogenous synthesis during myogenesis. In quantitative PCR studies, genes responsible for synthesizing were found be differentially regulated growth, repair, pathology. Although all Has (Has1, Has2, Has3) these models, only Has2 gene expression consistently associated with myogenic...
The material properties of natural tissues, such as skeletal muscle, are highly sophisticated and synthetically challenging to mimic. Using biomacromolecules functionalize self-assembled peptide (SAP) hydrogels has the potential increase utility these materials by more closely reproducing cellular environment. Here, demonstrate that a conserved co-assembly pathway can retain distinct function, biocompatible derivative Fmoc-FRGDF was co-assembled with either sulfated polysaccharide, fucoidan,...
In Duchenne muscular dystrophy (DMD), a dysregulated extracellular matrix (ECM) directly exacerbates pathology. Glucocorticoids are beneficial therapeutics in DMD, and have pleiotropic effects on the composition processing of ECM proteins other biological contexts. The synthesis remodelling transitional versican-rich is necessary for myogenesis; whether glucocorticoids modulate this not known. Here, versican expression were examined hindlimb diaphragm muscles from mdx dystrophin-deficient...
The cytokine-inducible SH2 domain containing protein (CISH) is the founding member of suppressor cytokine signaling (SOCS) family negative feedback regulators and has been shown to be a physiological regulator in immune cells. This study sought investigate novel functions for CISH outside system. Mice deficient were generated analyzed using range metabolic other parameters, including response high fat diet leptin administration. knockout mice possessed decreased body showed resistance...