A5015317111- Wound Healing and Treatments
- Vascular Malformations and Hemangiomas
- Chemical Synthesis and Analysis
- Dermatologic Treatments and Research
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Vascular Malformations Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Body Contouring and Surgery
- Click Chemistry and Applications
- Surgical Sutures and Adhesives
- Tumors and Oncological Cases
- Mesenchymal stem cell research
- Diagnosis and Treatment of Venous Diseases
- Vascular Tumors and Angiosarcomas
- Plant Surface Properties and Treatments
- Cancer and Skin Lesions
- Reconstructive Surgery and Microvascular Techniques
- Hair Growth and Disorders
- Sarcoma Diagnosis and Treatment
- Gene expression and cancer classification
- Facial Rejuvenation and Surgery Techniques
- Laser Applications in Dentistry and Medicine
- Periodontal Regeneration and Treatments
- Central Venous Catheters and Hemodialysis
Keio University
2015-2025
Keio University Hospital
2015-2023
Cleveland Clinic Lerner College of Medicine
2014-2017
Cleveland Clinic
2012
Abstract The proteoglycan versican is implicated in growth and metastases of several cancers. Here we investigated a potential contribution stromal to tumor angiogenesis. We initially determined expression by cancer cell lines. Among these, MDA-MB231 B16F10 had none minimal contrast Lewis lung carcinoma (LLC). Notably, tumors arising from these lines higher levels than the themselves suggesting host-derived stroma. In LLC-derived tumors, both stroma expressed at high levels. Thus, can make...
Background/Objectives: The therapeutic efficacy of percutaneous sclerotherapy (PS) for venous malformations (VMs) based on volumetric magnetic resonance imaging (MRI) measurements and its association with early post-treatment coagulation markers remains unexplored. This study evaluates the 5% monoethanolamine oleate (EO)-based PS in treating difficult-to-resect VMs using MRI investigates changes markers. Methods: post-hoc analysis utilized data from a prospective, open-label, multicenter...
To evaluate the effect and safety of sclerotherapy in patients with difficult-to-resect venous malformations treated ethanolamine oleate. This investigator-initiated clinical trial employed a multicenter, single-arm design was conducted Japan. Overall, 44 were categorized into two cohorts: 22 cystic-type diffuse-type malformations, including children (<15 years old). Adult received injections 5% oleate solution, double diluted contrast or normal saline, maximum dose 0.4 mL/kg. The same...
Summary ADAMTS9 is the most conserved member of a large family secreted metalloproteases having diverse functions. Adamts9 null mice die before gastrulation, precluding investigations its roles later in embryogenesis, adult or disease models. We therefore generated floxed allele to bypass embryonic lethality. In this mutant, unidirectional loxP sites flank exons 5–8, which encode catalytic domain, including protease active site. Mice homozygous for were viable, lacked an overt phenotype, and...
Adult mammalian wounds leave visible scars, whereas skin in developing mouse fetuses are scarless until a certain point development when complete regeneration occurs, including the structure of dermis and appendages. Analysis molecular mechanisms at this transition will provide clues for achieving wound healing. The fibroblast growth factor (FGF) family is key regulator inflammation fibrosis during We aimed to determine expression role FGF members fetal ICR were surgically wounded embryonic...
Fibrotic scars are common in both human and mouse skin wounds. However, wound-induced hair neogenesis the murine wounding models often results regenerative repair response. Herein, we aimed to uncover cellular functional heterogeneity dermis between fibrotic wound healing fates.The expression matrix of single-cell RNA sequencing (scRNA-seq) data dermal cells was filtered, normalized, scaled; underwent principal components analysis; further analyzed by Uniform Manifold Approximation...
Abstract Background Fibro-adipose vascular anomaly (FAVA) is a new entity of anomalies with somatic and mosaic gain-of-function mutations the phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha ( PIK3CA ). mutation excessively activates mammalian target rapamycin (mTOR) pathway, which promotes angiogenesis lymphangiogenesis. Histologically, FAVA composed intramuscular fibrous adipose tissues venous malformation (VM). Although sirolimus known as mTOR inhibitor has good...
Abstract Unlike adult mammalian wounds, early embryonic mouse skin wounds completely regenerate and heal without scars. Analysis of the underlying molecular mechanism will provide insights into scarless wound healing. Twist2 is an important regulator hair follicle formation biological patterning; however, it unclear whether plays a role in or appendage regeneration. Here, we aimed to elucidate expression its fetal ICR fetuses were surgically wounded on day 13 (E13), E15, E17, tissue samples...
Up to a certain developmental stage, fetus can completely regenerate wounds in the skin. To clarify mechanism of fetal skin regeneration, identifying when switches from fetal-type wound regeneration adult-type repair is necessary. We hypothesized that this switch occurs at several time points and complete requires epidermal-dermal interactions formation actin cables. compared normal morphology each stage. examined two parameters: epidermal texture dermal structure. found three-dimensional...
Treatment with growth factors is an effective method to promote wound healing. Several have been used in treatment, including basic fibroblast factor (bFGF) discovered the 1970s, platelet-derived (PDGF), epidermal (EGF), and bone morphogenetic protein (BMP). In 2001, Kaken Pharmaceutical Co., Ltd. launched Fiblast Spray Japan. This product a topical formulation containing recombinant human bFGF. It treat decubitus ulcers skin (burn leg ulcers). There increasing interest its stimulatory...
ADAM28 (a disintegrin and metalloproteinase 28) is overexpressed by carcinoma cells in non-small cell lung carcinomas (NSCLC) plays an important role cancer proliferation metastasis reactivation of insulin-like growth factor-1 (IGF-1) escaping from von Willebrand factor (VWF)-induced apoptosis through digestion IGF-binding protein-3 VWF, respectively. To aim for new target therapy NSCLC patients, we developed human neutralizing antibodies 211-12 211-14 against ADAM28, which showed IC50...
Chronic expanding haematoma (CEH) is a rare type of that enlarges slowly and continuously without coagulation. It can occur following surgery because shear stress-induced bleeding in the scar tissue between subcutaneous fat fascia. We present three cases large chronic CEH were successfully treated with triamcinolone injections. Three female patients developed at 9 months, 5 years, 6 respectively, after latissimus dorsi flap harvesting for breast reconstruction. Although condition did not...
Healthcare providers treating wounds have difficulties assessing the prognosis of patients with critical limb ischemia who had been discharged after complete healing major amputation wounds. The word "major" in "major amputation" gives impression "being more severe" than "minor amputation." Therefore, even if are healed amputation, they imagine that would be poorer minor amputation. We investigated diabetic nephropathy 2 years amputations. Those underwent dialysis as well following...
Summary: YAP (yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are part of a classical pathway that controls contact inhibition in the Hippo pathway. were recently reported to act as nuclear relays mechanical signals communicate extracellular matrix rigidity cell shape. However, role YAP/TAZ signaling keloid formation is unclear. Here, we used immunohistochemistry investigate expression nonaffected lesions. fibroblasts had greater tendency localize nucleus...
Abstract Wounds that occur in adults form scars due to fibrosis, whereas those embryos regenerate. If wound healing is mimicked adults, scarring can be reduced. We found mouse fetuses could regenerate tissues up embryonic day (E) 13, but visible remained thereafter. This regeneration pattern requires actin cable formation at the epithelial margin via activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK). Here, we investigated whether AMPK-activating effect salicylate,...
Wnt proteins secrete glycoproteins that are involved in various cellular processes to maintain homeostasis during development and adulthood. However, the expression role of wound healing have not been fully documented. Our previous studies shown that, an early-stage mouse fetus, no scarring occurred after cutaneous wounding, complete regeneration was achieved. In this study, localization a fetal-wound-healing model their associations with scar formation were analyzed. Wnt-related molecules...
Unlike adults, early developing fetuses can completely regenerate tissue, and replicating this could lead to the development of treatments reduce scarring. Mice epidermal structures, including wound healing patterns, are regenerated until embryonic day (E) 13, leaving visible scars thereafter. These patterns require actin cable formation at epithelial margin through AMP-activated protein kinase (AMPK) activation. We aimed investigate whether administration compound 13 (C13), a recently...
Cultured epidermal transplantation (JACE) is performed for giant congenital melanocytic nevus (GCMN), but there are few reports on its postoperative course and surgical content or indications. We aimed to investigate the of GCMN patients undergoing cultured autograft compare outcomes between 2 tissue resection methods.Twelve aged 0 months 8 years 9 were included in this single-center case series study. was at 19 sites patients' extremities trunks, after excision either by using an electric...
Scars developing after wrist cutting (a deliberate action of self-harm) have various patterns and are difficult to treat. In addition, they can occur at anatomically prominent sites easily recognized as caused by self-harm; thus, scars cause lifelong regrets. However, there no standard treatment guidelines for wounds inflicted through self-harm. This study aimed evaluate the effectiveness our novel technique using 90-degree rotated skin grafts, which were thinly collected a thickness 250 μm...