A5042463876- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Quantum Computing Algorithms and Architecture
- NF-κB Signaling Pathways
- T-cell and B-cell Immunology
- Quantum Information and Cryptography
- Quantum Mechanics and Applications
- Listeria monocytogenes in Food Safety
- Coding theory and cryptography
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- RNA regulation and disease
- PI3K/AKT/mTOR signaling in cancer
- Protein Tyrosine Phosphatases
- Immune cells in cancer
- Gut microbiota and health
- Parallel Computing and Optimization Techniques
- Wildlife Ecology and Conservation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Polynomial and algebraic computation
- RNA and protein synthesis mechanisms
- Pharmacological Effects of Natural Compounds
- IL-33, ST2, and ILC Pathways
Max Perutz Labs
2016-2025
University of Vienna
2016-2025
Vienna Biocenter
2005-2025
Oklahoma State University
2021
Vermont Fish & Wildlife Department
2020
United States Fish and Wildlife Service
2015-2016
University Medical Center Freiburg
2014
National University of Singapore
2013-2014
Centre for Quantum Technologies
2013-2014
Hungarian Academy of Sciences
2013
Significance Measuring activity patterns of microbes in their natural environment is essential for understanding ecosystems and the multifaceted interactions microorganisms with eukaryotes. In this study, we developed a technique that allows fast nondestructive measurements microbial communities on single-cell level. Microbial were amended heavy water (D 2 O), treatment does not change available substrate pool. After incubation, physiologically active cells are rapidly identified Raman...
Polymorphonuclear leukocytes are essential for host defense to infectious diseases. CCAAT/enhancer binding protein epsilon (C/EBP epsilon) is preferentially expressed in granulocytes and lymphoid cells. Mice with a null mutation C/EBP develop normally fertile but fail generate functional neutrophils eosinophils. Opportunistic infections tissue destruction lead death by 3-5 months of age. Furthermore, end-stage mice myelodysplasia, characterized proliferation atypical that efface the bone...
Abstract Different macrophage populations were investigated for their abilities to secrete tumor necrosis factor (TNF) and lyse TNF-susceptible cells. In this way we could demonstrate that TNF-secretion, although a feature of all activated populations, is no absolute requirement the killing TNF-sensitive Wehi 164 target. Macrophage cytotoxicity against cell but not TNF-resistant P815 mastocytoma, was completely inhibitable by specific anti-TNF serum also in absence measurable secreted TNF....
Secondary bacterial pneumonia leads to increased morbidity and mortality from influenza virus infections. What causes this susceptibility, however, is not well defined. Host defense infection relies only on immune resistance mechanisms but also the ability tolerate a given level of pathogen burden. Failure either or tolerance can contribute disease severity, making it hard distinguish their relative contribution. We employ coinfection mouse model Legionella pneumophila in which we separate...
Many intestinal pathogens, including Clostridioides difficile, use mucus-derived sugars as crucial nutrients in the gut. Commensals that compete with pathogens for such are therefore ecological gatekeepers healthy guts, and attractive candidates therapeutic interventions. Nevertheless, there is a poor understanding of which commensals mucin-derived situ well their potential to impede pathogen colonization. Here, we identify mouse gut utilize monosaccharides within complex communities using...
The animal and human intestinal mucosa secretes an assortment of compounds to establish a physical barrier between the host tissue contents, separation that is vital for health. Some pathogenic microorganisms as well members commensal microbiota have been shown be able break down these secreted compounds. Our understanding host-compound degradation by has limited knowledge about simplified model systems because difficulty in studying complex ecosystem vivo. In this study, we introduce...
Abstract Cells maintain the balance between homeostasis and inflammation by adapting integrating activity of intracellular signaling cascades, including JAK-STAT pathway. Our understanding how a tailored switch from to strong receptor-dependent response is coordinated remains limited. Here, we use an integrated transcriptomic proteomic approach analyze transcription-factor binding, gene expression in vivo proximity-dependent labelling proteins living cells under homeostatic interferon...
Abstract Immune cells need to sustain a state of constant alertness over lifetime. Yet, little is known about the regulatory processes that control fluent and fragile balance called homeostasis. Here we demonstrate JAK-STAT signaling, beyond its role in immune responses, major regulator cell We investigated JAK-STAT-mediated transcription chromatin accessibility across 12 mouse models, including knockouts all STAT factors TYK2 kinase. Baseline signaling was detected CD8 + T macrophages...
Interferon-stimulated gene factor 2 (ISGF2) was purified from HeLa cells treated with alpha interferon. The factor, a single polypeptide of 56 kilodaltons (kDa), bound both to the central 9 base pairs 15-base-pair interferon-stimulated response element (ISRE) that is required for transcriptional activation genes and PRD-I regulatory beta interferon gene. ISGF2 phosphoprotein, dephosphorylation in vitro reduced its DNA-binding activity. However, conditions changed amount did not change...
The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is transcriptionally induced in cells exposed to interferon of either type I (alpha [IFN-alpha] or II (IFN-gamma). promoter the GBP was cloned and found contain an IFN-alpha-stimulated response element, which mediated IFN-alpha. On basis transfection experiments with recombinant plasmids, two different elements were delineated. Both required obtain maximal IFN-gamma: element overlapping termed IFN-gamma activation site....