A5103022016- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Soil Carbon and Nitrogen Dynamics
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Diet and metabolism studies
- Microbial bioremediation and biosurfactants
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Wastewater Treatment and Nitrogen Removal
- Diabetes and associated disorders
- Microbial Fuel Cells and Bioremediation
- Phytase and its Applications
- Environmental DNA in Biodiversity Studies
- Particle physics theoretical and experimental studies
- Milk Quality and Mastitis in Dairy Cows
- Groundwater flow and contamination studies
- Pediatric health and respiratory diseases
- Identification and Quantification in Food
- Particle Detector Development and Performance
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- Fecal contamination and water quality
- Soil and Water Nutrient Dynamics
University of Bonn
2024
Helmholtz Zentrum München
2010-2019
Technical University of Munich
2008-2018
Excellence Cluster Universe
2018
Institute of Bioinformatics and Systems Biology
2015
Senckenberg Society for Nature Research
2015
Center for Environmental Health
2008-2014
Institute of Groundwater Ecology
2004-2008
Institute of Soil Science
2008
Genes of archaea encoding homologues ammonia monooxygenases have been found on a widespread basis and in large amounts almost all terrestrial marine environments, indicating that oxidizing (AOA) might play major role nitrification Earth. However, only one pure isolate this group from environment has so far obtained, demonstrating archaeal oxidation coupled with autotrophic growth similar to the bacterial counterparts. Here we describe cultivation isolation an AOA soil. It grows or urea as...
Soil pH is a major determinant of microbial ecosystem processes and potentially driver evolution, adaptation, diversity ammonia oxidizers, which control soil nitrification. Archaea are components communities contribute significantly to oxidation in some soils. To determine whether drives evolutionary adaptation community structure archaeal sequences amoA, key functional gene oxidation, were examined soils at global, regional, local scales. Globally distributed database clustered into 18...
Phosphorus (P) is an important macronutrient for all biota on earth but similarly a finite resource. Microorganisms play both sides of the fence as they effectively mineralize organic and solubilize precipitated forms soil phosphorus conversely also take up immobilize P. Therefore, we analysed role microbes in two beech forest soils with high low P content by direct sequencing metagenomic deoxyribonucleic acid. For inorganic solubilization, significantly higher microbial potential was...
The characterization of microbial community structure via 16S rRNA gene profiling has been greatly advanced in recent years by the introduction amplicon pyrosequencing. possibility barcoding gives opportunity to massively screen multiple samples from environmental or clinical sources for details. However, an on-going debate questions reproducibility and semi-quantitative rigour pyrotag sequencing, similar early days fingerprinting. In this study we demonstrate bacterial 454 sequencing over...
The plant microbial fuel cell (PMFC) is a technology in which living roots provide electron donor, via rhizodeposition, to mixed community generate electricity cell. Analysis and localisation of the necessary for gaining insight into competition donor PMFC. This paper characterises anode–rhizosphere bacterial Glyceria maxima (reed mannagrass) Electrochemically active bacteria (EAB) were located on root surfaces, but they more abundant colonising graphite granular electrode. Anaerobic...
Background High microbial diversity in the environment has been associated with lower asthma risk, particularly children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of mucosal surfaces airways. Methods DNA from mattress dust and nasal samples 86 school age was analyzed by 454 pyrosequencing 16S rRNA gene fragments. Based on operational taxonomic units (OTUs), bacterial composition were related farm exposure status. Results Farm positively...
The development of anti-islet cell autoimmunity precedes clinical type 1 diabetes and occurs very early in life. During this period, dietary factors strongly impact on the composition gut microbiome. At same time, microbiome plays a central role infant immune system. A functional model association between diet, microbial communities, can provide important new insights regarding pathogenesis diabetes. novel approach was developed to enable analysis an aggregation level single taxon classical...
Bioavailability of electron acceptors is probably the most limiting factor in restoration anoxic, contaminated environments. The oxidation contaminants such as aromatic hydrocarbons, particularly aquifers, often depends on reduction ferric iron or sulphate. We have previously detected a highly active fringe zone beneath toluene plume at tar-oil-contaminated aquifer Germany, where specialized community contaminant degraders codominated byDesulfobulbaceae andGeobacteraceae had established....
Microbial communities play an important role in cheese ripening and determine the flavor taste of different types to a large extent. However, under adverse conditions human pathogens may colonize samples during thus cause severe outbreaks diarrhoea other diseases. Therefore present study we investigated bacterial community structure three raw ewe's milk types, which are produced without application starter cultures from two production sites based on fingerprinting combination with next...
Climate change and air pollution, including ozone is known to affect plants might also influence the ragweed pollen, carry strong allergens. We compared transcriptome of pollen produced under ambient elevated by 454-sequencing. An enzyme-linked immunosorbent assay (ELISA) was carried out for major allergen Amb a 1. Pollen surface examined scanning electron microscopy attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR), phenolics were analysed high-performance...
The Osterseen Lake district in Bavaria consists of 19 small interconnected lakes that exhibit a pronounced trophic gradient from eutrophic to oligotrophic. It therefore presents unique model system address ecological questions regarding niche adaptation and Baas Becking's long standing hypothesis "everything is everywhere, but the environment selects". Here, we present first assessment microbial diversity these lakes. We sampled August December used 454 pyrosequencing 16S rRNA amplicons...
Microbial communities in soil provide a wide range of ecosystem services. On the small scale, nutrient rich hotspots developed from activities animals or plants are important drivers for composition microbial and their functional patterns. However, subsoil, spatial heterogeneity microbes with differing lifestyles has been rarely considered so far. In this study, phylogenetic bacterial archaeal microbiome based on 16S rRNA gene pyrosequencing was investigated compartments bulk soil,...
In a field experiment, the impact of repeated application antibiotic sulfadiazine (SDZ)-contaminated pig manure was assessed on functional microbial communities involved in ammonia and nitrite oxidation root-rhizosphere complexes (RRCs) diverse plants composing pasture. We surveyed abundance ammonia-oxidizing archaea (AOA) bacteria (AOB) as well Nitrobacter- Nitrospira-like nitrite-oxidizing (NOB) by quantitative PCR, diversity amoA AOA Nitrobacter-like nxrA amplicons using...
Alkane degrading microorganisms play an important role for the bioremediation of petrogenic contaminated environments. In this study, we investigated effects compost addition on abundance and diversity bacteria harboring alkane monooxygenase gene (alkB) in oil-contaminated soil originated from industrial zone Celje, Slovenia (Technosol). Soil without any amendments (control soil) amended with two composts differing their maturation stage nutrient availability, were incubated under controlled...
Background Changes in microbial community composition the lung of patients suffering from moderate to severe COPD have been well documented. However, knowledge about specific microbiome structures human associated with CT defined abnormalities is limited. Methods Bacterial derived brush samples lungs 16 different subtypes and 9 healthy subjects was analyzed using a cultivation independent barcoding approach applying 454-pyrosequencing 16S rRNA gene fragment amplicons. Results We could show...