A5089847134- Bacterial biofilms and quorum sensing
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Plant Pathogenic Bacteria Studies
- Biofuel production and bioconversion
- Gut microbiota and health
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial Peptides and Activities
- Infections and bacterial resistance
- Metabolomics and Mass Spectrometry Studies
- Plant and Biological Electrophysiology Studies
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Metalloenzymes and iron-sulfur proteins
- bioluminescence and chemiluminescence research
- Probiotics and Fermented Foods
- Slime Mold and Myxomycetes Research
- Metabolism and Genetic Disorders
- Pesticide and Herbicide Environmental Studies
- Antibiotic Resistance in Bacteria
Helmholtz Zentrum München
2010-2024
Ludwig-Maximilians-Universität München
2019-2024
Genomics (United Kingdom)
2016
German Center for Diabetes Research
2014
Center for Environmental Health
2010-2012
Institute of Groundwater Ecology
1994-2007
University of Wisconsin–Madison
1985-1991
University of Bayreuth
1983
University of Tübingen
1976-1981
ABSTRACT Given that a large proportion of the bacteria colonizing roots plants is capable producing N -acyl- l -homoserine lactone (AHL) molecules, it appears likely these bacterial pheromones may serve as signals for communication between cells different species. In this study, we have developed and characterized novel Gfp-based monitor strains allow in situ visualization AHL-mediated individual plant rhizosphere. For purpose, three AHL sensor plasmids respond to spectra molecules were...
Mutants of Azospirillum brasilense Sp Cd, resistant to 5-fluorotryptophan (FT) excreted 3-indoleacetic acid (IAA), i.e., auxin, producing up 16 μg/mL which was 30 times greater than the wild-type level. Under conditions nitrogen fixation, mutants IAA 1 μg/mL, 10 more wild type. However, none FT-resistant lipoferum RG 20a high levels IAA. This probably due differences in tryptophan and biosynthetic steps between A. strains. Some Cd showed a reduced feedback inhibition anthranilate synthetase...
We used computer-assisted microscopy at single cell resolution to quantify the in situ spatial scale of N-acylhomoserine lactone (AHL)-mediated cell-to-cell communication Pseudomonas putida colonized on tomato and wheat root surfaces. The results this quantification study close-to-natural surfaces challenge conventional view a quorum group requirement high densities for type bacterial communication. In image analysis indicated that effective 'calling distance' was most frequent 4–5 μm,...
OPINION article Front. Plant Sci., 08 April 2014Sec. Pathogen Interactions https://doi.org/10.3389/fpls.2014.00131
The plant microbial fuel cell (PMFC) is a technology in which living roots provide electron donor, via rhizodeposition, to mixed community generate electricity cell. Analysis and localisation of the necessary for gaining insight into competition donor PMFC. This paper characterises anode–rhizosphere bacterial Glyceria maxima (reed mannagrass) Electrochemically active bacteria (EAB) were located on root surfaces, but they more abundant colonising graphite granular electrode. Anaerobic...
The functional interaction of outer membrane proteins E. coli can be studied using phage and colicin receptors which are essential components penetration systems. uptake ferric iron in the form ferrichrome complex requires ton A B functions coli. gene product is receptor protein for T5 required together with function by phages T1 ø80 to infect cells M antibiotic albomycin, a structural analogue ferrichrome, kill cells. necessary complexed citrate. Iron enterochelin only transported presence...
The gut microbiota generates a huge pool of unknown metabolites, and their identification characterization is key challenge in metabolomics. However, there are still gaps on the studies chemical structures. In this investigation, an unusual class bacterial sulfonolipids (SLs) detected mouse cecum, which was originally found environmental microbes. We have performed detailed molecular level lipids by combining high-resolution mass spectrometry liquid chromatography analysis. Eighteen SLs that...
Abstract Eukaryotic organisms coevolved with microbes from the environment forming holobiotic meta-genomic units. Members of host-associated microbiomes have commensalic, beneficial/symbiotic, or pathogenic phenotypes. More than 100 years ago, Lorenz Hiltner, pioneer soil microbiology, introduced term ‘Rhizosphere’ to characterize observation that a high density saprophytic, beneficial, and are attracted by root exudates. The balance between these types decide about health host. Nowadays we...
The enrichment of nitrogen-fixing bacteria from the C4-fibre plants, Spartina pectinata, Miscanthus sinensis, sacchariflorus and Pennisetum purpureum, with nitrogen-free semi-solid media led to isolation Herbaspirillum-like strains among other diazotrophic bacteria. On basis physiological properties, phylogenetic analysis comparing 16S rDNA sequences DNA-DNA hybridization experiments chromosomal DNA new isolates could be grouped together in a species proposed name Herbaspirillum frisingense...
A large collection of bacterial strains, immunotrapped from soil and the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- genotypic parameters. Strains were grouped on (inter) repetitive extragenic palindromic DNA (REP) PCR profiles at intraspecies level. Pheno- characters assessed for representatives 13 different REP groups. nine groups constituting two physiological BIOLOG clusters fell in coherent DNA-DNA reassociation group Ochrobactrum anthropi. a...
Summary Two Gram‐negative, plant growth‐promoting rhizobacteria (PGPRs), denominated as M12 and M14, were classified by 16S rDNA sequencing Burkholderia graminis species. Both strains shown to produce a variety of N ‐acyl‐homoserine lactone (AHL) quorum sensing (QS) signalling molecules. The involvement these molecules in growth promotion the induction protection against salt stress was examined. AHL production evaluated vitro thin‐layer chromatography using biosensors, identity AHLs...
The biocontrol strain Pseudomonas putida IsoF, which was isolated from a tomato rhizosphere, is known N-acyl-homoserine lactone (AHL) producer with only one LuxI/LuxR-like quorum-sensing (QS) system. production and degradation of AHLs were analysed in different growth phases the bacterium. Using analytical tools ultra performance liquid chromatography high resolution MS, it possible to determine not various synthesized over time but also their products. 3-oxo-decanoyl-homoserine found be...
The antibiotic albomycin is transported into cells of Escherichia coli K-12 by the same uptake system as iron-supplying ferrichrome complex. iron-complexing hydroxamate moieties and are structurally similar. During phase rapid iron chelators were not found in cells. In order to understand activity albomycin, it was labeled with tritium presumed antibiotically active area radioactive sulfur. While label retained cells, part sulfur taken up concentrated 500-fold within cell. incorporated...
The majority of the marine algal species, though completing their life cycle in seawater, are rarely susceptible to fouling, making them an important source quorum sensing (QS) inhibitory substances. separation and characterization QS inhibitors crucial for any potential application. Thirty macroalgae were tested inhibition activity by using Chromobacterium violaceum CV026 as reporter strain, among them, Asparagopsis taxiformis showed antibacterial, well antiquorum, activities....
In our mouse model, gastric acid-suppression is associated with antigen-specific IgE and anaphylaxis development. We repeatedly observed non-responder animals protected from food allergy. Here, we aimed to analyse reasons for this protection. Ten out of 64 mice, subjected oral ovalbumin (OVA) immunizations under acid-suppression, were non-responders without OVA-specific or IgG1 elevation, indicating protection these non-responders, allergen challenges confirmed reduced antigen uptake lack...