A5029174102- Gut microbiota and health
- Reproductive tract infections research
- Metabolomics and Mass Spectrometry Studies
- Ferroptosis and cancer prognosis
- Mass Spectrometry Techniques and Applications
- Probiotics and Fermented Foods
- Cancer, Lipids, and Metabolism
- Drug Transport and Resistance Mechanisms
- Cholesterol and Lipid Metabolism
- Urinary Tract Infections Management
- Analytical Chemistry and Chromatography
- Pituitary Gland Disorders and Treatments
- Protist diversity and phylogeny
- Semiconductor materials and devices
- Marine Sponges and Natural Products
- Drug-Induced Hepatotoxicity and Protection
- Cell death mechanisms and regulation
- Soil Moisture and Remote Sensing
- Dental Trauma and Treatments
- Polyamine Metabolism and Applications
- Microbial Community Ecology and Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Seaweed-derived Bioactive Compounds
- Phytoestrogen effects and research
- Sexual Differentiation and Disorders
Helmholtz Zentrum München
2013-2023
Center for Environmental Health
2019
Chemnitz University of Technology
2006
Leibniz Institute for Solid State and Materials Research
2005
Ferroptosis is an iron-dependent form of regulated cell death linking iron, lipid, and glutathione levels to degenerative processes tumor suppression. By performing a genome-wide activation screen, we identified cohort genes antagonizing ferroptotic death, including GTP cyclohydrolase-1 (GCH1) its metabolic derivatives tetrahydrobiopterin/dihydrobiopterin (BH4/BH2). Synthesis BH4/BH2 by GCH1-expressing cells caused lipid remodeling, suppressing ferroptosis selectively preventing depletion...
Global HIV-1 treatment would benefit greatly from safe herbal medicines with scientifically validated novel anti-HIV-1 activities. The root extract the medicinal plant Pelargonium sidoides (PS) is licensed in Germany as medicine EPs®7630, numerous clinical trials supporting its safety humans. Here we provide evidence multiple cell culture experiments that PS displays potent activity. We show protects peripheral blood mononuclear cells and macrophages infection various X4 R5 tropic strains,...
Ferroptosis is a regulated cell death modality that occurs upon iron-dependent lipid peroxidation. Recent research has identified many regulators induce or inhibit ferroptosis; yet, regulatory processes and networks remain to be elucidated. In this study, we performed chemical genetics screen using small molecules with known mode of action two agonists the nuclear receptor Farnesoid X Receptor (FXR) suppress ferroptosis, but not apoptosis necroptosis. We demonstrate in liver cells high FXR...
Chlamydia trachomatis is an obligate intracellular pathogen responsible for loss of eyesight through trachoma and millions cases annually sexually transmitted diseases. The bacteria develop within a membrane-bounded inclusion. They lack enzymes several biosynthetic pathways, including those to make some phospholipids, exploit their host compensate. Three-dimensional fluorescence microscopy demonstrates that small organelles the host, peroxisomes, are translocated into inclusion found...
We present concepts of complexity, and complex chemistry in systems subjected to biotic abiotic transformations, introduce analytical possibilities disentangle chemical complexity into its elementary parts as a global integrated approach termed <italic>systems analytics</italic>.
The Chlamydiae are a highly successful group of obligate intracellular bacteria, whose members remarkably diverse, ranging from major pathogens humans and animals to symbionts ubiquitous protozoa. While their infective developmental stage, the elementary body (EB), has long been accepted be completely metabolically inert, it recently shown sustain some activities, including uptake amino acids protein biosynthesis. In current study, we performed an in-depth characterization metabolic...
Isoprene is a small lipophilic molecule with important functions in plant protection against abiotic stresses. Here, we studied the lipid composition of thylakoid membranes and chloroplast ultrastructure isoprene-emitting (IE) nonisoprene-emitting (NE) poplar (Populus × canescens). We demonstrated that total amount monogalactosyldiacylglycerols, digalactosyldiacylglycerols, phospholipids, fatty acids reduced chloroplasts when isoprene biosynthesis blocked. A significantly lower unsaturated...
Effective growth and replication of obligate intracellular pathogens depend on host cell metabolism. How this is connected to mitochondrial function has not been studied so far. Recent studies suggest that bacteria such as Chlamydia pneumoniae enhanced in a low oxygen environment, arguing for particular mechanistic role the respiration controlling progeny. Metabolic changes C. infected epithelial cells were analyzed under normoxic (O2≈20%) hypoxic conditions (O2<3%). We observed infection...
Marine algae represent an important source of novel natural products. While their bioactive potential has been studied to some extent, limited information is available on marine from the Red Sea. This study aimed at broad discovery new bioactivities a collection twelve macroalgal species Central We used imaging-based High-Content Screening (HCS) with diverse spectrum cellular markers for detailed cytological profiling fractionated algal extracts. The profiles 3 out 60 fractions clustered...
RATIONALE The ionization of neutral diacylglycerols (DAGs) by electrospray mass spectrometry (ESI‐MS) is challenging compared with other lipid classes which possess ionic head group conjugations. Although ESI‐MS the method choice in lipidomic analysis, it questionable whether all can be efficiently ionized this method. Actually, various lipids were not detected (due to poor ionization) many studies claimed comprehensively describe profiles. Since are precursors for biosynthesis most classes,...
Reduced nitrogen species are key nutrients for biological productivity in the oceans. Ammonium is often present low and growth-limiting concentrations, albeit peaks occur during collapse of algal blooms or via input from deep sea upwelling riverine inflow. Autotrophic phytoplankton exploit ammonium by storing intracellularly. In contrast, strategy heterotrophic bacterioplankton to acquire less well understood. This study revealed marine bacterium Phaeobacter inhibens DSM 17395, a Roseobacter...
Eradication of Helicobacter pylori usually consists a 7-day course triple therapy including metronidazole or amoxicillin plus clarithromycin proton pump inhibitor. We report about rare adverse event Hp eradication in patient with moderate chronic and active pangastritis. Shortly after the end treatment cholestatic hepatitis occurred which was most likely related to clarithromycin, perhaps enhanced by amoxicillin. Since liver dysfunction self-limited, no further required. In summary,...
ABSTRACT Objective Ferroptosis is a regulated cell death modality that occurs upon iron-dependent lipid peroxidation. The recent decade of research has uncovered many regulators driving ferroptosis as well cellular gatekeepers preventing ferroptosis. Yet, processes and networks remain to be elucidated. Methods results In this study, we performed chemical screen using small molecules with known mode action identified two agonists (Turofexorate Fexaramine) the nuclear receptor Farnesoid X...
<b>Background:</b> The use of probiotic bacteria has been proposed for prevention allergic diseases. However, clinical trials have given inconsistent results. This may be explained by the complexity crosstalk between bacteria, host´s microbiota and immune cells. identification a biochemically defined, bioactive substances from could circumvent these problems. We previously detected metabolite D-Tryptophan (D-Trp) that had immunomodulatory properties in <i>in vitro</i> screening assays....
<b>Background:</b> Previously, D-Tryptophan has been identified as soluble bacterial molecule with Th2-lowering activity <i>in vitro</i> from prebiotic supernatants using bioassay-guided fractionation. However, the vivo</i> effects of on Th2-mediated disease are unknown. Here, we studied oral supplementation allergic airway inflammation in mice. <b>Methods:</b> Six - eight weeks old female Balb/c mice received 50mM D-tryptophan (Sigma Aldrich, Germany) dissolved filtered drinking water or...
Dieser Beitrag legt den Fokus auf die parodontale Therapie furkationsbeteiligter Molaren. Der Therapieentscheid ist anspruchsvoll, da zahlreiche Faktoren berucksichtigt werden mussen. Therapeutisches Ziel Wiederherstellung der uneingeschrankten Plaquekontrolle in allen durch Attachmentverlust entstandenen unzuganglichen Nischen im Bereich Furkation. Die Tunnelierung stellt eine mogliche Therapieoption dar. Je nach Indik ation erfolgt sie nicht chirurgisch (geschlossenes Scaling und...