A5007385561- RNA modifications and cancer
- Effects of Radiation Exposure
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Retinal Development and Disorders
- Peptidase Inhibition and Analysis
- Chemotherapy-induced cardiotoxicity and mitigation
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Plant Stress Responses and Tolerance
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Photosynthetic Processes and Mechanisms
- RNA regulation and disease
- Retinal Diseases and Treatments
- Pesticide and Herbicide Environmental Studies
- Microbial bioremediation and biosurfactants
- Bone and Dental Protein Studies
- Pluripotent Stem Cells Research
- Plant responses to elevated CO2
- Radiation Therapy and Dosimetry
- RNA Interference and Gene Delivery
Helmholtz Zentrum München
2016-2025
Center for Environmental Health
2017-2025
Center for Integrated Protein Science Munich
2018-2020
Ferroptosis is an iron-dependent form of regulated cell death linking iron, lipid, and glutathione levels to degenerative processes tumor suppression. By performing a genome-wide activation screen, we identified cohort genes antagonizing ferroptotic death, including GTP cyclohydrolase-1 (GCH1) its metabolic derivatives tetrahydrobiopterin/dihydrobiopterin (BH4/BH2). Synthesis BH4/BH2 by GCH1-expressing cells caused lipid remodeling, suppressing ferroptosis selectively preventing depletion...
Adipocyte-derived extracellular vesicles (AdEVs) are membranous nanoparticles that convey communication from adipose tissue to other organs. Here, delineate their role as messengers with glucoregulatory nature, we paired fluorescence AdEV-tracing and SILAC-labeling (phospho)proteomics, revealed AdEVs transfer functional insulinotropic protein cargo into pancreatic β-cells. Upon transfer, AdEV proteins were subjects for phosphorylation, augmented GPCR/cAMP/PKA signaling by increasing total...
To date, the proteomic profiling of Müller cells, dominant macroglia retina, has been hampered because absence suitable enrichment methods. We established a novel protocol to isolate native, intact cells from adult murine retinae at excellent purity which retain in situ morphology and are well suited for analyses. Two different strategies sample preparation - an StageTips (iST) subcellular fractionation approach including cell surface protein were used quantitative liquid chromatography-mass...
Radiation is a highly efficient therapy in squamous head and neck carcinoma (HNSCC) treatment. However, local recurrence metastasis are common complications. Recent evidence shows that cancer-cell-derived exosomes modify tumour cell movement metastasis. In this study, we link radiation-induced changes of to their ability promote migration recipient HNSCC cells. We demonstrate isolated from irradiated donor cells boost the motility BHY FaDu. Molecular data identified enhanced AKT-signalling,...
Article9 April 2018Open Access Transparent process Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury Jesica Frik Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University Munich, Germany Institute for Stem Cell Research, Helmholtz Center Instituto de Biotecnología y Biología Molecular, UNLP-CONICET, La Plata, Argentina Search more papers by this author Juliane Merl-Pham Research Unit Protein Science, Nikolaus Plesnila Stroke...
RNA-binding proteins (RBPs) and long non-coding RNAs (lncRNAs) are key regulators of gene expression, but their joint functions in coordinating cell fate decisions poorly understood. Here we show that the expression activity RBP TDP-43 isoform lncRNA Neat1, scaffold nuclear compartment "paraspeckles," reciprocal pluripotent differentiated cells because cross-regulation. In cells, represses formation paraspeckles by enhancing polyadenylated short Neat1. also promotes pluripotency regulating...
Epidemiological data from radiotherapy patients show the damaging effect of ionizing radiation on heart and vasculature. The endothelium is main target damage contributes essentially to development cardiac injury. However, molecular mechanisms behind radiation-induced endothelial dysfunction are not fully understood. In present study, 10-week-old C57Bl/6 mice received local X-ray doses 8 or 16 Gy were sacrificed after weeks; controls sham-irradiated. microvascular cells isolated tissue using...
Ferroptosis is an iron-dependent form of cell death driven by biochemical processes that promote oxidation within the lipid compartment. Calcium (Ca2+) a signaling molecule in diverse cellular such as migration, neurotransmission, and death. Here, we uncover crucial link between ferroptosis Ca2+ through identification novel tetraspanin MS4A15. MS4A15 localizes to endoplasmic reticulum, where it blocks depleting luminal stores reprogramming membrane phospholipids ferroptosis-resistant...
Multiple cell types secrete exosome-like extracellular vesicles (ELVs) to the environment. Pathological conditions can produce characteristic changes vesicle cargo. We investigated if ionizing radiation is capable of inducing in protein and microRNA (miRNA) cargo ELVs.Whole blood samples from healthy donors were irradiated with 2 Gy gamma rays then peripheral mononuclear cells plasma separated residual co-cultivated for 24 h. The released ELVs collected by differential ultracentrifugation...
Lung fibrosis is characterized by excessive deposition of extracellular matrix (ECM), in particular collagens, fibroblasts the interstitium. Transforming growth factor-β1 (TGF-β1) alters expression many (ECM) components produced fibroblasts, but such changes ECM composition as well modulation collagen post-translational modification (PTM) levels have not been comprehensively investigated. Here, we performed mass spectrometry (MS)-based proteomics analyses to assess deposited cultured lung...
Exploring adaptive strategies by which microorganisms function and survive in low-energy natural environments remains a grand goal of microbiology, may help address prime challenge the 21st century: degradation man-made chemicals at low concentrations ("micropollutants"). Here we explore physiological adaptation maintenance energy requirements herbicide (atrazine)-degrading microorganism (Arthrobacter aurescens TC1) while concomitantly observing mass transfer limitations directly...
Astrocyte-to-neuron conversion is a promising avenue for neuronal replacement therapy. Neurons are particularly dependent on mitochondrial function, but how well mitochondria adapt to the new fate unknown. Here, we determined comprehensive proteome of cortical astrocytes and neurons, identifying about 150 significantly enriched proteins each cell type, including transporters, metabolic enzymes, cell-type-specific antioxidants. Monitoring their transition during reprogramming revealed late...
Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen to uninfected systemic leaves following an initial localized infection. In Arabidopsis (Arabidopsis thaliana), local infection with virulent or avirulent strains of Pseudomonas syringae pv tomato generates long-distance SAR signals travel from locally infected distant through the phloem establish this study, proteomics approach was used identify proteins accumulate in exudates...
// Omid Azimzadeh 1 , Tamara Azizova 2 Juliane Merl-Pham 3 Vikram Subramanian Mayur V. Bakshi Maria Moseeva Olga Zubkova Stefanie M. Hauck Nataša Anastasov Michael J. Atkinson 1, 4 Soile Tapio Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH), Institute of Radiation Biology, Neuherberg, Germany Southern Urals Biophysics Institute, Russian Federation, Ozyorsk, Russia Unit Protein Science, Munich, Chair Technical University Correspondence to: Tapio, email:...
Hybrid-poplar tree plantations provide a source for biofuel and biomass, but they also increase forest isoprene emissions. The consequences of increased emissions include higher rates tropospheric ozone production, increases in the lifetime methane, atmospheric aerosol all which affect global energy budget and/or lead to degradation air quality. Using RNA interference (RNAi) suppress emission, we show that this trait, is thought be required tolerance abiotic stress, not high photosynthesis...
Advancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for specimens. Steps along the method design involve determination optimization of several bioanalytical parameters such as selectivity, sensitivity, accuracy, precision. In a joint effort, eight laboratories belonging to MSCoreSys initiative including CLINSPECT-M, MSTARS, DIASyM, SMART-CARE consortia performed longitudinal round-robin study assess...
During brain development, neural stem cells (NSCs) must balance self-renewal with differentiation and ensure lineage progression. To identify novel regulators of NSCs during neurogenesis, we isolated by FACS from the mouse cerebral cortex ganglionic eminence at mid-neurogenesis, birth, when gliogenesis starts in both, but neurogenesis only continues latter region. RNA-seq ATAC-seq revealed major transcriptional chromatin changes between these stages identified TGFB-Induced Homeobox Factor 2...
Enteroviruses (EVs), such as Coxsackievirus B5 (CVB5), are linked to pancreatic beta cell autoimmunity in type 1 diabetes (T1D). CVB5 infection of cells blocks their cap-dependent translation but spares cap-independent insulin secretory granule (SG) cargoes, including major T1D autoantigens. Despite this, SG stores depleted. We show that protease 2A rapidly depletes Golgi-associated sorting factor GGA2 due its short half-life. Immunostaining pancreas sections from recent-onset donors...
Protein S-nitrosylation, the covalent binding of nitric oxide (NO) to protein cysteine residues, is one main mechanisms NO signaling in plant and animal cells. Using a combination biotin switch assay label-free LC-MS/MS analysis, we revealed S-nitroso-proteome woody model Populus x canescens. Under normal conditions, constitutively S-nitrosylated proteins poplar leaves calli comprise all aspects primary secondary metabolism. Acute ozone fumigation was applied elicit ROS-mediated changes...
Epidemiological data from patients undergoing radiotherapy for thoracic tumors clearly show the damaging effect of ionizing radiation on cardiovascular system. The long-term impairment heart function and structure after local high-dose irradiation is associated with systemic inflammatory response, contraction impairment, microvascular damage, cardiac fibrosis. goal present study was to investigate molecular mechanisms involved in this process. C57BL/6J mice received a single X-ray dose 16 Gy...
Researchers have been examining the biological function(s) of isoprene in isoprene-emitting (IE) species for two decades. There is overwhelming evidence that leaf-internal increases thermotolerance plants and protects them against oxidative stress, thus mitigating a wide range abiotic stresses. However, mechanisms stress mitigation by are still under debate. Here, we assessed impact on emission nitric oxide (NO) S-nitroso-proteome IE non-isoprene-emitting (NE) gray poplar (Populus ×...
Abstract The RNA-binding protein PURA has been implicated in the rare, monogenetic, neurodevelopmental disorder Syndrome. binds both DNA and RNA associated with various cellular functions. Only little is known about its main roles molecular pathways affected upon depletion. Here, we show that predominantly located cytoplasm, where it to thousands of mRNAs. Many these transcripts change abundance response encoded proteins suggest a role for immune responses, mitochondrial function, autophagy...
Identification of interactors is a major goal in cell biology. Not only protein-protein but also protein-carbohydrate interactions are high relevance for signal transduction biological systems. Here, we aim to identify novel interacting binding partners the β-galactoside-binding proteins galectin-1 (Gal-1) and galectin-3 (Gal-3) relevant context eye disease proliferative vitreoretinopathy (PVR). PVR one most common failures after retinal detachment surgeries characterized by migration,...