A5058227137- Effects of Radiation Exposure
- Mitochondrial Function and Pathology
- Radiation Dose and Imaging
- Radiation Therapy and Dosimetry
- Chemotherapy-induced cardiotoxicity and mitigation
- Cancer, Hypoxia, and Metabolism
- Cardiac Imaging and Diagnostics
- Extracellular vesicles in disease
- Advanced Proteomics Techniques and Applications
- Cancer-related cognitive impairment studies
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Cardiovascular Disease and Adiposity
- Circular RNAs in diseases
- Neurogenesis and neuroplasticity mechanisms
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Anesthesia and Neurotoxicity Research
- Cytokine Signaling Pathways and Interactions
- Connexins and lens biology
- Air Quality and Health Impacts
- Photoacoustic and Ultrasonic Imaging
- Medical Imaging Techniques and Applications
Helmholtz Zentrum München
2015-2024
Technical University of Munich
2016-2023
Center for Environmental Health
2007-2015
Institute of Bioinformatics and Systems Biology
2007-2012
Federal Office for Radiation Protection
2005-2011
Stockholm University
1991-1994
University of Konstanz
1991-1992
Columbia University
1991
Uppsala University
1986-1990
Backround Radiation therapy treatment of breast cancer, Hodgkin's disease or childhood cancers expose the heart to high local radiation doses, causing an increased risk cardiovascular in survivors decades after treatment. The mechanisms that underlie damage remain poorly understood so far. Previous data show impairment mitochondrial oxidative metabolism is directly linked development disease. Methodology/Principal findings In this study, radiation-induced vivo effects on cardiac proteome and...
Radiation is a highly efficient therapy in squamous head and neck carcinoma (HNSCC) treatment. However, local recurrence metastasis are common complications. Recent evidence shows that cancer-cell-derived exosomes modify tumour cell movement metastasis. In this study, we link radiation-induced changes of to their ability promote migration recipient HNSCC cells. We demonstrate isolated from irradiated donor cells boost the motility BHY FaDu. Molecular data identified enhanced AKT-signalling,...
Chronic low-dose ionizing radiation induces cardiovascular disease in human populations but the mechanism is largely unknown. We suggested that chronic exposure may induce endothelial cell senescence associated with vascular damage vivo. investigated whether causing a change onset of cells vitro. Indeed, when exposed to continuous rate gamma (4.1 mGy/h), primary umbilical vein (HUVECs) initiated much earlier than nonirradiated control cells. changes protein expression HUVECs before and...
Epidemiological data from radiotherapy patients show the damaging effect of ionizing radiation on heart and vasculature. The endothelium is main target damage contributes essentially to development cardiac injury. However, molecular mechanisms behind radiation-induced endothelial dysfunction are not fully understood. In present study, 10-week-old C57Bl/6 mice received local X-ray doses 8 or 16 Gy were sacrificed after weeks; controls sham-irradiated. microvascular cells isolated tissue using...
Accidental nuclear scenarios lead to environmental contamination of unknown level. Immediate radiation-induced biological responses that trigger processes leading adverse health effects decades later are not well understood. A comprehensive proteomic analysis provides a promising means identify and quantify the initial damage after radiation exposure. Early changes in cardiac tissue C57BL/6 mice exposed total body irradiation were studied, using dose relevant both intentional accidental...
Epidemiological evidence suggests that low doses of ionising radiation (≤1.0 Gy) produce persistent alterations in cognition if the exposure occurs at a young age. The mechanisms underlying such are unknown. We investigated long-term effects total body gamma on neonatally exposed NMRI mice molecular and cellular level to elucidate neurodegeneration. Significant spontaneous behaviour were observed 2 4 months following single 0.5 or 1.0 Gy exposure. Alterations brain proteome, transcriptome,...
The etiology of radiation-induced cardiovascular disease (CVD) after chronic exposure to low doses ionizing radiation is only marginally understood. We have previously shown that a low-dose rate (4.1 mGy/h) causes human umbilical vein endothelial cells (HUVECs) prematurely senesce. now show dose 2.4 mGy/h also able trigger premature senescence in HUVECs, primarily indicated by loss growth potential and the appearance senescence-associated markers ß-galactosidase (SA-ß-gal) p21. In contrast,...
Formalin-fixed paraffin-embedded (FFPE) tissue has recently gained interest as an alternative to fresh/frozen for retrospective protein biomarker discovery. However, during the fixation process, proteins undergo degradation and cross-linking, making conventional analysis technologies problematic. In this study, we have compared several extraction separation methods of in FFPE tissues. Incubation sections at high temperature with a novel buffer (20 mM Tris-HCl, pH 8.8, 2% SDS, 1%...
Multiple cell types secrete exosome-like extracellular vesicles (ELVs) to the environment. Pathological conditions can produce characteristic changes vesicle cargo. We investigated if ionizing radiation is capable of inducing in protein and microRNA (miRNA) cargo ELVs.Whole blood samples from healthy donors were irradiated with 2 Gy gamma rays then peripheral mononuclear cells plasma separated residual co-cultivated for 24 h. The released ELVs collected by differential ultracentrifugation...
Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses protein cargo EVs may lead to discovery biomarkers for diseases. However, a comprehensive MS-based proteomics analysis, an optimal lysis is required. Six methods extraction from secreted by head and neck cell line BHY were compared. Commercial radioimmunoprecipitation assay (RIPA) buffer outperformed...