A5081529617- Hedgehog Signaling Pathway Studies
- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Radiation Dose and Imaging
- Cancer-related Molecular Pathways
- Connexins and lens biology
- Molecular Biology Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Genetic and rare skin diseases.
- Cancer-related cognitive impairment studies
- Cancer and Skin Lesions
- PARP inhibition in cancer therapy
- Mitochondrial Function and Pathology
- Skin Protection and Aging
- Plant Genetic and Mutation Studies
- Cancer Cells and Metastasis
- Genetic Mapping and Diversity in Plants and Animals
- Nuclear Physics and Applications
- Hippo pathway signaling and YAP/TAZ
- Chemical Reactions and Isotopes
National Agency for New Technologies, Energy and Sustainable Economic Development
2015-2024
Instituto Nacional do Câncer
2012
Ministry of Health
2012
Tecnologia Energia Ambiente Materiali (Italy)
2003-2010
ENEA Casaccia Research Centre
2004-2009
University of Wisconsin–Madison
1994
Istituto Superiore di Sanità
1991-1993
The central dogma of radiation biology, that biological effects ionizing are a direct consequence DNA damage occurring in irradiated cells, has been challenged by observations genetic/epigenetic changes occur unexposed "bystander cells" neighboring directly-hit due to cell-to-cell communication or soluble factors released cells. To date, the vast majority these described cell-culture systems, while vivo validation and assessment consequences within an organism remain uncertain. Here, we...
Abstract Loss-of-function mutations in Patched (Ptch1) are implicated constitutive activation of the Sonic hedgehog pathway human basal cell carcinomas (BCCs), and inherited Ptch1 underlie nevus syndrome which a typical feature is multiple BCC occurring with greater incidence portals radiotherapy. Mice one copy inactivated show increased susceptibility to spontaneous tumor development hypersensitivity radiation-induced tumorigenesis, providing an ideal vivo model study pathologies associated...
Epidemiological evidence suggests that low doses of ionising radiation (≤1.0 Gy) produce persistent alterations in cognition if the exposure occurs at a young age. The mechanisms underlying such are unknown. We investigated long-term effects total body gamma on neonatally exposed NMRI mice molecular and cellular level to elucidate neurodegeneration. Significant spontaneous behaviour were observed 2 4 months following single 0.5 or 1.0 Gy exposure. Alterations brain proteome, transcriptome,...
Therapeutic irradiation of pediatric and adult patients can profoundly affect neurogenesis, cognitive impairment manifests as a deficit in hippocampal-dependent functions. Age plays major role susceptibility to radiation, younger children are at higher risk decay when compared adults. Cranial affects hippocampal neurogenesis by induction DNA damage neural progenitors, through the disruption neurogenic microenvironment, defective integration newborn neurons into neuronal network. Our goal...
// Mariateresa Mancuso 1 , Emanuela Pasquali Ignacia Braga-Tanaka III 2 Satoshi Tanaka Alessandro Pannicelli 3 Paola Giardullo 4, 5 Simonetta Pazzaglia Soile Tapio 6 Michael J. Atkinson Anna Saran Laboratory of Radiation Biology and Biomedicine, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Rome, Italy Institute for Environmental Sciences (IES), Rokkasho, Aomori, Japan Technical Unit Energetic Efficiency, ENEA, 4 Department Physics,...
Abstract The Hedgehog (Hh) pathway is essential for embryonic development and tissue homeostasis. Aberrant Hh signaling may occur in a wide range of human cancers, such as medulloblastoma, the most common brain malignancy childhood. Here, we identify endoplasmic reticulum aminopeptidase 1 (ERAP1), key regulator innate adaptive antitumor immune responses, previously unknown player pathway. We demonstrate that ERAP1 binds deubiquitylase enzyme USP47, displaces USP47-associated βTrCP,...
The last decades have seen increased concern about the possible effects of low to moderate doses ionizing radiation (IR) exposure on cognitive function. An interdisciplinary group experts (biologists, epidemiologists, dosimetrists and clinicians) in this field gathered together framework European MELODI workshop non-cancer IR summarise state knowledge topic elaborate research recommendations for future studies area. Overall, there is evidence from both biology epidemiology, though a better...
Chronic inflammatory bowel disorders (IBD) are idiopathic diseases associated with altered intestinal permeability, which in turn causes an exaggerated immune response to enteric antigens a genetically susceptible host. A rise psych cognitive disorders, such as anxiety and depression, has been observed IBD patients. We here report investigations on model of chemically induced experimental colitis by oral administration sodium dextran sulfate (DSS) C57BL/6 mice. investigate, vivo, the...
Moving from the evidence that activation of type 4 metabotropic glutamate (mGlu4) receptors inhibits proliferation and promotes differentiation cerebellar granule cell neuroprogenitors, we examined expression function mGlu4 in medulloblastoma cells. were expressed 46 60 human samples. Expression varied relation to histotype (nodular desmoplastic>classic≫large-cell anaplastic) was inversely related tumor severity, spreading, recurrence. also found D283med, D341med, DAOY lines, where...
Patched1 heterozygous mice ( Ptch1 +/− ) are useful for basal cell carcinoma (BCC) studies, being remarkably susceptible to BCC induction by ultraviolet or ionizing radiation. Analogously, skin carcinogenesis-susceptible (Car-S) elective studies of papilloma and squamous (SCC) induction. We previously reported a striking effect gender on in mice, with total resistance females; likewise, Car-S females show increased tumor relative males. Here, we investigated the protective role endogenous...
In humans, in utero exposure to ionising radiation results an increased prevalence of neurological aberrations, such as small head size, mental retardation and decreased IQ levels. Yet, the association between early damaging events long-term neuronal anomalies remains largely elusive.Mice were exposed different X-ray doses, ranging 0.0 1.0 Gy, at embryonic days (E) 10, 11 or 12 subjected behavioural tests weeks age. Underlying mechanisms irradiation E11 further unravelled using magnetic...
Medulloblastoma, the most common brain tumor in childhood, appears to originate from cerebellar granule cell precursors (GCPs), located external granular layer (EGL) of cerebellum. The antiproliferative gene PC3 (Tis21/BTG2) promotes neurogenesis by inducing GCPs shift proliferation differentiation. To assess whether can prevent neoplastic transformation and medulloblastoma development, we crossed transgenic mice conditionally expressing (TgPC3) with Patched1 heterozygous (Ptc(+/-)), a model...
Molecular communication between irradiated and unirradiated neighbouring cells initiates radiation-induced bystander effects (RIBE) out-of-field (abscopal) which are both an example of the non-targeted (NTE) ionising radiation (IR). Exosomes small membrane vesicles endosomal origin newly identified mediators NTE. Although exosome-mediated changes well documented in therapy oncology, there is a lack knowledge regarding role exosomes derived from inside outside field early delayed induction...
Many genes controlling neuronal development also regulate adult neurogenesis. We investigated in vivo the effect of Shh signaling activation on patterning and neurogenesis hippocampus behavior Patched1 heterozygous mice (Ptch1+/-). demonstrated for first time, that Ptch1+/- exhibit morphological, cellular molecular alterations dentate gyrus (DG), including elongation reduced width DG as well deregulations at multiple steps during lineage progression from neural stem cells to neurons. By...
Abstract We examined the effects of hair cycle phase on basal cell carcinoma (BCC) tumorigenesis induced by radiation in mice lacking one Patched allele (Ptc1neo67/+). Our results show that Ptc1neo67/+ mouse skin irradiated early anagen is highly susceptible to tumor induction, as a 3.2-fold incidence visible BCC-like tumors was observed anagen-irradiated compared with telogen-irradiated mice. Microscopic nodular were also enhanced irradiation during active hair-follicle growth phases....