A5049859883- MicroRNA in disease regulation
- Telomeres, Telomerase, and Senescence
- Circular RNAs in diseases
- Bioinformatics and Genomic Networks
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Thyroid Cancer Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Cancer-related Molecular Pathways
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Clusterin in disease pathology
- Effects of Radiation Exposure
- Molecular Biology Techniques and Applications
- Cardiac Imaging and Diagnostics
- Histone Deacetylase Inhibitors Research
Helmholtz Zentrum München
2013-2022
Institute of Biological and Medical Imaging
2021
Technical University of Munich
2003-2013
Klinikum rechts der Isar
2013
Karolinska Institutet
2011
National Institutes of Health
2006
National Cancer Institute
2006
Max Planck Institute of Biochemistry
2003-2004
Ferroptosis is an iron-dependent form of regulated cell death linking iron, lipid, and glutathione levels to degenerative processes tumor suppression. By performing a genome-wide activation screen, we identified cohort genes antagonizing ferroptotic death, including GTP cyclohydrolase-1 (GCH1) its metabolic derivatives tetrahydrobiopterin/dihydrobiopterin (BH4/BH2). Synthesis BH4/BH2 by GCH1-expressing cells caused lipid remodeling, suppressing ferroptosis selectively preventing depletion...
Indoleamine-2,3-dioxygenase (IDO) inhibitors have entered clinical trials based on their ability to restore anti-tumor immunity in preclinical studies. However, the mechanisms leading constitutive expression of IDO human tumors are largely unknown. Here we analyzed pathways mediating cancer. IDO-positive tumor cells and tissues showed basal phosphorylation acetylation STAT3 as evidenced by western blotting immunoprecipitation. Inhibition IL-6 or using siRNA and/or pharmacological reduced...
In BriefO'Leary et al. find a long non-coding RNA called PARTICLE that is overexpressed following irradiation.PARTICLE represses tumor suppressor MAT2A via triplex formation and interaction with the polycomb repressor complex.PARTICLE also acts as cytosolic scaffold for in preparation exosomal transport from cell.
Radiation is a highly efficient therapy in squamous head and neck carcinoma (HNSCC) treatment. However, local recurrence metastasis are common complications. Recent evidence shows that cancer-cell-derived exosomes modify tumour cell movement metastasis. In this study, we link radiation-induced changes of to their ability promote migration recipient HNSCC cells. We demonstrate isolated from irradiated donor cells boost the motility BHY FaDu. Molecular data identified enhanced AKT-signalling,...
There is evidence that the extent of G2/M arrest following irradiation correlated with tumour cell survival and hence therapeutic success. We studied regulation cellular response to radiation treatment by miR-21-mediated modulation cycle progression in breast cancer cells analysed miR-21 expression tissue samples long-term follow up. The levels were quantified (qRT-PCR) a panel 86 cases invasive carcinomas relation metastasis free survival. radiosensitivity human after was determined...
A detailed understanding of the mechanisms that determine variable cellular sensitivity to radiation is needed for improved therapy as well identification individuals with innate hypersensitivity. MicroRNAs (miRNAs) are a class small non-coding RNAs post-transcriptionally regulate protein expression. Alterations in miRNA expression patterns response ionizing have been shown, but there almost no data describing functional impact these changes. We report here results studies on roles miRNAs...
MiR-221/-222 are frequently overexpressed in breast cancer and associated with increased malignancy. The specific modification of microRNAs (miRNAs) expression could be a promising strategy therapy, leading to the suppression tumourigenic processes tumour cells. expressions were analysed 86 tissues by quantitative RT–PCR tested for correlation immunohistochemistry data clinical follow-up. In vitro assays conducted using human cell lines lentiviral overexpression miR-221/-222. tissues,...
Radiation resistance presents a challenge to the effective treatment of cancer. If therapeutic compounds were capable resensitizing resistant tumours then concurrent chemo-radiation could be used overcome radiation resistance. We have developed phenotypic assay investigate response breast cancer cells grown in 3D-microtissue spheroids combinations and established chemotherapeutic drugs. The effects quantified by real time high content imaging GFP detection area over 14 days. Ten drugs tested...
// Omid Azimzadeh 1 , Tamara Azizova 2 Juliane Merl-Pham 3 Vikram Subramanian Mayur V. Bakshi Maria Moseeva Olga Zubkova Stefanie M. Hauck Nataša Anastasov Michael J. Atkinson 1, 4 Soile Tapio Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH), Institute of Radiation Biology, Neuherberg, Germany Southern Urals Biophysics Institute, Russian Federation, Ozyorsk, Russia Unit Protein Science, Munich, Chair Technical University Correspondence to: Tapio, email:...
Background Anaplastic lymphoma kinase (ALK)-positive anaplastic large cell is characterized by the t(2;5) chromosomal translocation, resulting in expression of a fusion protein formed nucleophosmin (NPM) and ALK. Recently, we reported abnormal transcription factor CCAAT/enhancer binding protein-beta (C/EBPβ) ALK-positive lymphomas, demonstrated its dependence on NPM-ALK activity.Design Methods In this study, role C/EBPβ proliferation survival lymphomas was investigated, as well mechanism...
Abstract Background Although lentiviral transduction methods are widely used, their broader application is dependent upon the optimization of efficiency for a broad range cell types. In present study, we focus on evaluation two chemical classes with respect to ability increase without cytotoxicity. Methods We compared activity adjuvants that already used lentivirus delivery novel selected basis and physical characteristics. Results The poloxamer synperonic F108 demonstrated superior...
Gonadotroph adenomas comprise 15-40% of all pituitary tumors, are usually non-functioning and often large invasive at presentation. Surgery is the first-choice treatment, but complete resection not always achieved, leading to high recurrence rates. As gonadotroph poorly respond conventional pharmacological therapies, novel treatment strategies needed. Their identification has been hampered by our incomplete understanding molecular pathogenesis these tumors. Recently, we demonstrated that...
Pancreatic cancer has a poor prognosis. New treatment options are urgently required to improve patient outcomes. One promising new class of anticancer drugs synthetic histone deacetylase inhibitors (HDACi) which modulate chromatin structure and gene expression by blocking deacetylation. In this study, we aimed at comparing the in vitro capacities HDACi SAHA CUDC-101 increase radiosensitivity human pancreatic tumor cell lines. Therefore, three lines (Su.86.86, MIA Paca-2, T3M-4) were treated...
Abstract Background Breast cancer is the most common malignancy in female patients worldwide. Because of its heterogeneity terms prognosis and therapeutic response, biomarkers with potential to predict survival or assist making treatment decisions breast are essential for an individualised therapy. Epigenetic alterations genome cells, such as changes DNA methylation pattern, could be a novel marker important role initiation progression cancer. Method RNA-seq datasets from The Cancer Genome...
Log in or Register Subscribe to journalSubscribe Get new issue alertsGet alerts Enter your Email address: Wolters Kluwer Health may email you for journal and information, but is committed maintaining privacy will not share personal information without express consent. For more please refer our Privacy Policy. eTOC Secondary Logo Journal All Articles Images Videos Podcasts Blogs Advanced Search Toggle navigation Login & Issues Current IssuePrevious IssuesPublished Ahead-of-Print Authors...
C/EBPβ (CCAAT enhancer binding protein) is a transcription factor that plays crucial role in survival and transformation of ALK+ anaplastic large cell lymphoma (ALCL). The aim this study was to identify the downstream targets responsible for ALK-mediated oncogenesis. knocked down ALCL lines with C/EBPβ-shRNA, followed by gene expression profiling (GEP). GEP analysis revealed reproducible signature genes were significantly regulated C/EBPβ. Classification into biological categories...
A 3D microtissues using T47D and JIMT-1 cells were generated to analyze tissue-like response of breast cancer after combined human epidermal growth factor receptor 2 (HER2)-targeted treatment radiation. Following lentiviral knockdown HER2, we compared rate alterations 2D monolayers, microtissues, mouse xenografts. Additionally, model therapeutic strategies, treated HER2-depleted trastuzumab (anti-HER2 antibody) in combination with irradiation. Comparison HER2 corresponding controls revealed...
Glioblastoma stem-like cells (GSLCs) in glioblastoma limit effective treatment and promote therapeutic resistance tumor recurrence. Using a combined radiation drug-screening platform, we tested the combination of histone deacetylase inhibitor (HDACi) MAPK/ERK kinase (MEKi) with to predict efficacy against GSLCs. To mimic phenotype, glioblastoma-derived spheres were used treated HDACi (MS-275) MEKi (TAK-733 or trametinib) 4 Gy irradiation. The sphere-forming ability after radiochemotherapy...
RNA interference is a powerful tool for the functional analysis of proteins by specific gene knockdown. In this study, we devised rapid and efficient way to screen suitable siRNA sequences subsequently employ them knockdown in usually hard-to-transfect lymphoid cell lines, using self-inactivating lentiviral vector. Two with different half-lives were chosen, cyclin D1 STAT3. A lacZ reporter fusion assay was used identify highly effective sequences. Only molecules more than 85% efficiency...