A5033413132- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced Proteomics Techniques and Applications
- Advanced Combustion Engine Technologies
- Political Economy and Marxism
- Radiopharmaceutical Chemistry and Applications
- Extracellular vesicles in disease
- Social and Demographic Issues in Germany
- Advanced machining processes and optimization
- Chemical synthesis and alkaloids
- Flexible and Reconfigurable Manufacturing Systems
- Retinal Development and Disorders
- Welding Techniques and Residual Stresses
- Advanced Surface Polishing Techniques
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Laser and Thermal Forming Techniques
- Microbial Metabolic Engineering and Bioproduction
- Social Policies and Healthcare Reform
- Immunotherapy and Immune Responses
- Metabolomics and Mass Spectrometry Studies
- Health and Medical Studies
- Innovation, Technology, and Society
- Quality and Supply Management
Graz University of Technology
2016-2024
Technische Universität Berlin
2014-2022
Helmholtz Zentrum München
2019-2022
Amazon (Germany)
2022
Wilhelm Löhe University for Applied Sciences
2016-2021
Wayne State University
2020-2021
Vorarlberg University of Applied Sciences
2021
Swim Across America
2019
Institute of Automation
2014-2019
Comprehensive Cancer Center Erlangen
2019
Chemical cross-linking combined with mass spectrometry has proven useful for studying protein-protein interactions and protein structure, however the low density of cross-link data so far precluded its use in determining structures de novo. Cross-linking been typically limited by chemical selectivity standard reagents that are commonly used cross-linking. We have implemented a heterobifunctional reagent, sulfosuccinimidyl 4,4'-azipentanoate (sulfo-SDA), combining traditional...
Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses protein cargo EVs may lead to discovery biomarkers for diseases. However, a comprehensive MS-based proteomics analysis, an optimal lysis is required. Six methods extraction from secreted by head and neck cell line BHY were compared. Commercial radioimmunoprecipitation assay (RIPA) buffer outperformed...
<div class="section abstract"><div class="htmlview paragraph">In order to combat the predicted consequences of climate change, major efforts are required in all industries. The use hydrogen combustion engines can make a valuable contribution as CO2-free bridging technology. A challenge development suitable process is high ignitibility with an impact on stability due anomalies. avoidance such phenomena highest relevance for ensuring long-term stable engine operation far possible....
Irreversible T cell exhaustion limits the efficacy of programmed death 1 (PD-1) blockade. We observed that dual CD40-TLR4 stimulation within a single tumor restored PD-1 sensitivity and this regimen triggered systemic tumor-specific CD8+ response. This approach effectively treated established tumors in diverse syngeneic cancer models, effect was dependent on injected tumor, indicating were converted into necessary components therapy. Strikingly, associated with absence exhausted PD-1hi cells...
Abstract Certain protein‐design calculations involve using an experimentally determined high‐resolution structure as a template to identify new sequences that can adopt the same fold. This approach has led successful design of many novel, well‐folded, native‐like proteins. Although any atomic‐resolution serve in such calculations, most designs have used crystal structures. Because there are proteins for which structures not available, it is interest whether nuclear magnetic resonance (NMR)...
Quantification of nanoparticle (NP) uptake in cells or tissues is very important for safety assessment. Often, electron microscopy based approaches are used this purpose, which allow imaging at high resolution. However, precise quantification NP numbers and remains challenging. The aim study was to present a novel approach, that combines NPs individual together with resolution their intracellular distribution on focused ion beam/ scanning (FIB/SEM) slice view approaches.We quantified...
Accurately predicted contacts allow to compute the 3D structure of a protein. Since solution space native residue-residue contact pairs is very large, it necessary leverage information identify relevant regions space, i.e. correct contacts. Every additional source can contribute narrowing down candidate regions. Therefore, recent methods combined evolutionary and sequence-based as well physicochemical information. We develop new predictor (EPSILON-CP) that goes beyond current by combining...
Abstract To counteract the anticipated consequences of climate change, significant efforts are required across all industries. The use hydrogen in internal combustion engines can make a valuable contribution as carbon-neutral bridging technology. One challenge developing suitable process is high ignition propensity hydrogen, impacting stability due to anomalies. Avoiding such phenomena utmost relevance ensure long-term stable engine operation. Oil entry into chamber impact occurrence...
ABSTRACT Hybrid approaches combine computational methods with experimental data. The information contained in the data can be leveraged to probe structure of proteins otherwise elusive methods. Compared methods, structures produced by hybrid exhibit some degree validation. In spite these advantages, most have not yet been validated blind tests, hampering their development. Here, we describe first test a specific cross‐link based method CASP. This was coordinated CASP organizers and utilized...
Intestinal absorption of the water-soluble vitamins biotin and pantothenic acid is carrier mediated involves sodium-dependent multivitamin transporter (SMVT; product SLC5A6 gene). We recently observed that intestinal-specific (conditional) knockout mouse Slc5a6 gene (SMVT-cKO) associated with growth retardation, development spontaneous severe inflammation, abnormal histology in large intestine, altered gut permeability, early death. Our aim this study was to examine possibility...
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, detailed understanding of the normal response to necessary predict risk and development strategies for protection. One component that continuously exposed during therapeutic irradiation circulating population peripheral blood mononuclear cells (PBMC). PBMCs are highly sensitive ionizing (IR); however, little known about how IR affects PBMC on systemic level. It was aim this study investigate whether capable...
Increased levels of the chaperone protein GRP78 have been implicated in poorer outcomes cancer therapy. We therefore explored functional connection between expression and development radioresistance metastatic behavior HNSCC.The association gene survival HNSCC patients was examined using TCGA database. The influence ionizing radiation on cell lines, their secreted extracellular vesicles (EV) non-irradiated EV-recipient cells investigated by Western Blot FACS. consequences chemical inhibition...
Abstract LEW rats were treated intravenously with recombinant rat interferon-γ (IFN-γ) for 3 days to achieve intravascular accumulation, proliferation, and activation of monocytes. Monocytes, defined by their expression the ED1, ED9, Ox41 antigens, recovered from vasculature perfusion PBS/EDTA, subsequently depleted erythrocytes granulocytes Percoll density gradient centrifugation, analyzed flow cytometry immunocytology. In untreated control-infused specified pathogen-free (SPF) rats,...
Hybrid methods combine experimental data and computational modeling to analyze protein structures that are elusive structure determination. To spur the development of hybrid methods, we propose test them in context CASP experiment would like invite groups participate this initiative.
A new automated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) sample spotting technique that allows the integration of MALDI preparation in workflow combinatorial polymer research is described. The performed utilizing a commercially available synthetic robot and was first evaluated with standards known composition later on used for monitoring living cationic ring-opening polymerization 2-ethyl-2-oxazoline. carried out as multiple layer approach,...
We introduce a novel contact prediction method that achieves high accuracy by combining evolutionary and physicochemical information about native contacts. obtain from multiple-sequence alignments predicted ab initio protein structures. These structures represent low-energy states in an energy landscape thus capture the encoded function. Such are likely to contain contacts, even if their overall fold is not native. To differentiate non-native contacts those structures, we develop graph-based...
We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and crosslink identification software Xi. Sequential enhances peptide selective shortening long tryptic peptides. demonstrate our simple 12-fraction protocol for multi-protein complexes cell lysates, quantitative analysis, high-density crosslinking, without requiring specific crosslinker features. This overall approach reveals dynamic protein-protein...
Cancer immunotherapy with dendritic cell-tumor cell fusion hybrids induces polyclonal stimulation against a variety of tumor antigens, including unknown antigens. Hybrid cells can prime CTLs, which subsequently develop antitumor responses. The aim this study was to enhance the known effect hybrid vaccination (HC-Vacc) and hybrid-primed adoptive T-cell therapy (HC-ACT) using poorly immunogenic Lewis lung carcinoma (LLC1) model. strategy used combination double HC-Vacc alternating HC-ACT...