A5025718257- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Nanopore and Nanochannel Transport Studies
- Genomics and Phylogenetic Studies
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Venous Thromboembolism Diagnosis and Management
- RNA Research and Splicing
- Cancer, Stress, Anesthesia, and Immune Response
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Long-Term Effects of COVID-19
- Amyotrophic Lateral Sclerosis Research
- Microfluidic and Bio-sensing Technologies
- Immune cells in cancer
- Intensive Care Unit Cognitive Disorders
- Interdisciplinary Research and Collaboration
- Asthma and respiratory diseases
Johns Hopkins University
2020-2024
Johns Hopkins Medicine
2020-2024
Technical University of Munich
2016-2023
LMU Klinikum
2018-2021
Ludwig-Maximilians-Universität München
2016-2021
Committee on Publication Ethics
2019
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
ABSTRACT Extracellular vesicles (EVs) are intercellular communicators with key functions in physiological and pathological processes have recently garnered interest because of their diagnostic therapeutic potential. The past decade has brought about the development commercialization a wide array methods to isolate EVs from serum. Which subpopulations captured strongly depends on isolation method, which turn determines how suitable resulting samples for various downstream applications. To...
Abstract Septic shock is a common medical condition with mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators sepsis urgently needed. High‐throughput technologies assessing circulating micro RNA s represent an important tool biomarker identification, but the blood‐compartment specificity these mi has not yet been investigated. We characterized profiles from serum exosomes, total blood...
Small RNA-Seq has emerged as a powerful tool in transcriptomics, gene expression profiling and biomarker discovery. Sequencing cell-free nucleic acids, particularly microRNA (miRNA), from liquid biopsies additionally provides exciting possibilities for molecular diagnostics, might help establish disease-specific signatures. The complexity of the small workflow, however, bears challenges biases that researchers need to be aware order generate high-quality data. Rigorous standardization...
This report summarises the presentations and activities of ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among key messages was broad agreement about importance biospecimen science. Much greater attention needs to be paid towards provenance collected samples. The workshop also highlighted clear gaps our knowledge pre-analytical factors that alter vesicles (EVs). future utility certified standards for credentialing instruments software, analyse...
Abstract Cell‐free microRNAs (miRNAs) are transferred in disease state including inflammatory lung diseases and often packed into extracellular vesicles (EVs). To assess their suitability as biomarkers for community‐acquired pneumonia (CAP) severe secondary complications such sepsis, we studied patients with CAP (n = 30), sepsis 65) healthy volunteers 47) subdivided a training 67) validation 75) cohort. After precipitating crude EVs from sera, associated small RNA was profiled by...
Background Extracellular vesicles (EVs) are mediators of cell-to-cell communication in inflammatory lung diseases. They function as carriers for miRNAs which regulate mRNA transcripts and signaling pathways after uptake into recipient cells. We investigated whether associated with circulating EVs immunologic processes COVID-19. Methods prospectively studied 20 symptomatic patients COVID-19 pneumonia, mechanically ventilated severe (severe acute respiratory corona virus-2 syndrome, ARDS)...
Extracellular vesicles (EVs), including microvesicles and exosomes, deliver bioactive cargo mediating intercellular communication in physiological pathological conditions. EVs are increasingly investigated as therapeutic agents targets, but also disease biomarkers. However, a definite consensus regarding EV isolation methods is lacking, which makes it intricate to standardize research practices eventually reach desirable level of data comparability. In our study, we performed an...
Abstract Editor’s Perspective What We Already Know about This Topic Article Tells Us That Is New Background Extracellular vesicles and their microRNA cargo are crucial facilitators of malignant cell communication could mediate effects anesthetics on tumor biology during cancer resection. The authors performed a proof-of-concept study to demonstrate that propofol sevoflurane have differential vesicle-associated microRNAs influence signaling pathways involved in progression metastasis. Methods...
Tumor-derived extracellular vesicles (EVs) have been associated with immune evasion and tumor progression. We show that the RNA-sensing receptor RIG-I within cells governs biogenesis immunomodulatory function of EVs. Cancer-intrinsic activation releases EVs, which mediate dendritic cell maturation T antitumor immunity, synergizing checkpoint blockade. Intact RIG-I, autocrine interferon signaling, GTPase Rab27a in are required for immunostimulatory Active intrinsic signaling composition EV...
ABSTRACT Extracellular vesicles (EVs) play central physiological and pathophysiological roles in intercellular communication. Biomarker studies addressing disorders such as cardiovascular diseases often focus on circulating microRNAs (miRNAs) may, depending the type of disease clinic routine, utilise patient specimens sampled from arterial or venous blood vessels. Thus, it is essential to test whether miRNA profiles depend respective sampling site. We assessed potential differences cell‐free...
Abstract Background Active smoking has been reported among 7% of teenagers worldwide, with ages ranging from 13 to 15 years. An epidemiological study suggested that preconceptional paternal is associated adolescent obesity in boys. We developed a murine model before conception investigate the molecular causes changes offspring’s phenotype. Method Male and female C57BL/6J mice were exposed increasing doses mainstream cigarette smoke (CS) onset puberty for 6 weeks mated room air (RA) controls....
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, detailed understanding of the normal response to necessary predict risk and development strategies for protection. One component that continuously exposed during therapeutic irradiation circulating population peripheral blood mononuclear cells (PBMC). PBMCs are highly sensitive ionizing (IR); however, little known about how IR affects PBMC on systemic level. It was aim this study investigate whether capable...
Hypoxia, or low oxygen tension, is frequently found in highly proliferative solid tumors such as anaplastic thyroid carcinoma (ATC) and believed to promote resistance chemotherapy radiation. Identifying hypoxic cells for targeted therapy may thus be an effective approach treating aggressive cancers. Here, we explore the potential of well-known hypoxia-responsive microRNA (miRNA) miR-210-3p a cellular extracellular biological marker hypoxia. We compare miRNA expression across several ATC...
In colorectal cancer (CRC) with microsatellite instability (MSI), >90% of cases are affected by inactivating frameshift mutations transforming growth factor β receptor type 2 (TGFBR2). TGFBR2 deficiency is considered to drive MSI tumor progression abrogating downstream TGF‑β signaling. This pathway can alter the expression coding and non‑coding RNAs, including microRNAs (miRNAs), which also present in extracellular vesicles (EVs) as post‑transcriptional modulators gene expression. our...
Extracellular vesicles (EVs) such as exosomes are key regulators of intercellular communication that can be found in almost all bio fluids. Although studies the last decade have made great headway discerning role EVs many physiological and pathophysiological processes, bioavailability impact dietary their cargo still remain to elucidated. Due its widespread consumption high content EV-associated microRNAs proteins, a major focus this field has been set on bovine milk colostrum. Despite...
Today, pancreatic cancer is the seventh leading cause of cancer-related deaths worldwide with a five-year overall survival rate less than 7%. Only 15–20% patients are eligible for curative intent surgery at time diagnosis. Therefore, neoadjuvant treatment regimens have been introduced in order to downsize tumor by chemotherapy and radiotherapy. To further increase efficacy radiotherapy, novel molecular biomarkers urgently needed define subgroup who would benefit most from MicroRNAs (miRNAs)...
Purpose: Radioresistance in pancreatic cancer patients remains a critical obstacle to overcome.Understanding the molecular mechanisms underlying radioresistance may achieve better response radiotherapy and thereby improving poor treatment outcome.The aim of present study was elucidate leading by detailed characterization isogenic radioresistant radiosensitive cell lines.Methods: The human lines, Panc-1 MIA PaCa-2 were repeatedly exposed radiation generate (RR) lines.The surviving cells...
Abstract Background Progranulin is a widely expressed pleiotropic growth factor with central regulatory effect during the early immune response in sepsis. signaling has not been systematically studied and compared between sepsis, community-acquired pneumonia (CAP), COVID-19 sterile systemic inflammatory (SIRS). We delineated molecular networks of progranulin by next-generation sequencing (NGS), determined plasma concentrations quantified diagnostic performance to differentiate...
Triple-negative breast cancer (TNBC) is a particularly aggressive subtype of with limited options for clinical intervention. As many solid tumors, TNBC known to promote invasiveness and metastasis by secreting extracellular vesicles (EVs) capable modulating the behaviour recipient cells. Recent investigations have demonstrated that high expression levels glucocorticoid receptor (GR) in are linked therapy resistance, higher recurrence rates increased mortality. In addition activating...