A5083186525- Connective Tissue Growth Factor Research
- Liver physiology and pathology
- Fibroblast Growth Factor Research
- Systemic Sclerosis and Related Diseases
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- TGF-β signaling in diseases
- Connective tissue disorders research
- Pancreatitis Pathology and Treatment
- Proteoglycans and glycosaminoglycans research
- Biomarkers in Disease Mechanisms
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Gastrointestinal disorders and treatments
- Phagocytosis and Immune Regulation
- Liver Disease and Transplantation
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Growth Hormone and Insulin-like Growth Factors
- Liver Diseases and Immunity
- Virus-based gene therapy research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive Biology and Fertility
- Circular RNAs in diseases
Nationwide Children's Hospital
2016-2025
The Ohio State University Wexner Medical Center
2010-2025
The Ohio State University
2009-2019
Jilin University
2014-2015
Park Plaza Hospital
2012
Level (Czechia)
2012
Columbus Community Clinical Oncology Program
2012
Columbus Oncology and Hematology Associates
2012
Children’s Institute
2003-2007
Friedrich-Alexander-Universität Erlangen-Nürnberg
2005
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Connective tissue growth factor (CCN2) drives fibrogenesis in hepatic stellate cells (HSC). Here we show that CCN2 up-regulation fibrotic or steatotic livers, culture-activated ethanol-treated primary mouse HSC, is associated with a reciprocal down-regulation of microRNA-214 (miR-214). By using protector reporter assays to investigate the 3'-untranslated region (UTR) mRNA, found induction expression HSC by fibrosis-inducing stimuli was due reduced miR-214, which otherwise inhibited directly...
A proposal is put forth to unify the nomenclature of CCN family secreted, cysteine rich regulatory proteins. In order their description in literature, CCN1 (CYR61), CCN2 (CTGF), CCN3 (NOV), CCN4 (WISP-1), CCN5 (WISP-2), and CCN6 (WISP-3) constitute a matricellular proteins that regulate cell adhesion, migration, proliferation, survival, differentiation, at least part through integrin mediated mechanisms. This endorsed by International Society will serve eliminate confusion from multiple...
Connective tissue growth factor (CCN2, also known as CTGF) is a matricellular protein that appears to play an important role in hepatic stellate cell (HSC)-mediated fibrogenesis. After signal peptide cleavage, the full-length CCN2 molecule comprises four structural modules (CCN21–4) and susceptible proteolysis by HSC yielding isoforms comprising essentially 3 4 (CCN23–4) or module alone (CCN24). In this study we show rat activated are capable of adhesion all three via binding integrin αvβ3,...
Uterine growth factors are potential effector molecules in embryo signaling pathways. Pig uterine luminal flushings contained a heparin-binding factor (HBGF) that required 0.8 M NaCl for elution from heparin columns and was termed HBGF-0.8. This factor, which heat- acid-labile of Mr 10,000 as assessed by gel filtration, stimulated DNA synthesis fibroblasts smooth muscle cells but not endothelial cells. Two forms HBGF-0.8, HBGF-0.8-P1 HBGF-0.8-P2, exhibited differential properties....
Matrix metalloproteinases (MMPs) exert both pro- and antiangiogenic functions by the release of cytokines or proteolytically generated angiogenic inhibitors from extracellular matrix basement membrane remodeling. In Mmp2−/− mouse neovascularization is greatly reduced, but mechanistic aspects this remain unclear. Using isotope-coded affinity tag labeling proteins analyzed multidimensional liquid chromatography tandem mass spectrometry we explored proteome differences between cells those...
Rationale: An increase in the number of mononuclear phagocytes lung biopsies from patients with idiopathic pulmonary fibrosis (IPF) worsens prognosis. Chemokines that recruit phagocytes, such as CC chemokine ligand 2 (CCL2), are elevated bronchoalveolar lavage (BAL) fluid (BALF) IPF. However, little attention is given to role phagocyte survival and recruitment factor, macrophage colony-stimulating factor (M-CSF), fibrosis.Objectives: To investigate M-CSF fibrosis.Methods: Wild-type,...
A hallmark of liver fibrosis is the activation hepatic stellate cells (HSC), which results in their production fibrotic molecules, a process that largely regulated by connective tissue growth factor (CCN2). CCN2 increasingly expressed during HSC because diminished expression microRNA-214 (miR-214), product dynamin 3 opposite strand (DNM3os) directly suppresses mRNA. We show an E-box miR-214 promoter binds basic helix-loop-helix transcription factor, Twist1, drives and suppression. Twist1 was...
The lack of approved therapies for hepatic fibrosis seriously limits medical management patients with chronic liver disease. Since extracellular vesicles (EVs) function as conduits intercellular molecular transfer, we investigated if EVs from healthy individuals have anti-fibrotic properties. Hepatic fibrogenesis or in carbon tetrachloride (CCl4)- thioacetic acid-induced injury models male female mice were suppressed by serum normal (EVN) but not fibrotic (EVF). CCl4-treated undergoing EVN...
In recent months, four different systems have been reported in the literature which CCN2 transgenes were individually expressed podocytes, hepatocytes, cardiomyocytes or respiratory epithelial cells to achieve overexpression in, respectively, kidney, liver, heart, lung. These transgenic provided valuable information about contribution of fibrosis vivo and begun reveal complexities underlying mechanisms involved. On one hand, studies these animals revealed that does not necessarily lead...
Atherosclerotic blood vessels overexpress connective tissue growth factor (CTGF) mRNA, but the role of CTGF in atherosclerosis remains controversial. To assess hypothesis that is involved atherosclerotic plaque progression, we investigated protein expression and distribution different types morphology.Serial cross-sections 45 human carotid plaques were immunohistochemically analyzed for presence protein, neovascularization (von Willebrand factor), macrophages (CD68), T cells (CD3). The...
Stimulation of endogenous β-cell expansion could facilitate regeneration in patients with diabetes. In mice, connective tissue growth factor (CTGF) is expressed embryonic β-cells and adult during periods expansion. We discovered that embryos CTGF necessary for proliferation, increased promotes proliferation immature (MafA−) insulin-positive cells. overexpression, under nonstimulatory conditions, does not increase proliferation. this study, we tested the ability to promote after partial...