A5045237366- Nanoparticle-Based Drug Delivery
- Atherosclerosis and Cardiovascular Diseases
- Electrospun Nanofibers in Biomedical Applications
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Characterization and Applications of Magnetic Nanoparticles
- Erythrocyte Function and Pathophysiology
- Connective Tissue Growth Factor Research
- Bone Tissue Engineering Materials
- Cerebrovascular and Carotid Artery Diseases
- Coronary Interventions and Diagnostics
- Nanoparticles: synthesis and applications
- Angiogenesis and VEGF in Cancer
- Biomarkers in Disease Mechanisms
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Cardiovascular Disease and Adiposity
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Hydrogels: synthesis, properties, applications
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Nanoplatforms for cancer theranostics
- Graphene and Nanomaterials Applications
Else Kröner-Fresenius-Stiftung
2015-2024
Universitätsklinikum Erlangen
2015-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2013-2024
Max Planck Institute for Molecular Biomedicine
2022
Nanomed (Brazil)
2018
Creative Research Enterprises (United States)
2015
Leibniz Supercomputing Centre
2011
Nuremberg Hospital
2005-2011
Bavarian Academy of Sciences and Humanities
2011
Natural and Medical Sciences Institute
2004
Due to the relatively poor cell-material interaction of alginate hydrogel, alginate-gelatin crosslinked (ADA-GEL) hydrogel was synthesized through covalent crosslinking di-aldehyde (ADA) with gelatin that supported cell attachment, spreading and proliferation. This study highlights evaluation physico-chemical properties ADA-GEL hydrogels different compositions compared in form films. Moreover, vitro on investigated by using normal human dermal fibroblasts. Viability, proliferation...
Background: Rising criticism of currently available contrast agents for magnetic resonance imaging, either due to their side effects or limited possibilities in terms functional evoked the need safer and more versatile agents. We previously demonstrated suitability novel dextran-coated superparamagnetic iron oxide nanoparticles (SPION Dex ) biomedical applications safety biocompatibility. Methods: In present study, we investigated size-dependent cross-linking process these particles as well...
Abstract: The promising potential of superparamagnetic iron oxide nanoparticles (SPIONs) in various nanomedical applications has been frequently reported. However, although many different synthesis methods, coatings, and functionalization techniques have described, not core-shell SPION drug delivery systems are available for clinicians at the moment. Here, bovine serum albumin was adsorbed onto lauric acid-stabilized SPIONs. agglomeration behavior, zeta potential, their dependence on...
Abstract: Iron oxide-based contrast agents have been in clinical use for magnetic resonance imaging (MRI) of lymph nodes, liver, intestines, and the cardiovascular system. Superparamagnetic iron oxide nanoparticles (SPIONs) high potential as a agent MRI, but no intravenous oxide-containing are currently approved imaging. The aim our work was to analyze hemocompatibility immuno-safety new type dextran-coated SPIONs (SPIONdex) characterize these with ultra-high-field MRI. Key parameters...
A facile and flexible approach for the integration of biomimetically branched microvasculature within bulk hydrogels is presented. For this, sacrificial scaffolds thermoresponsive poly(2-cyclopropyl-2-oxazoline) (PcycloPrOx) are created using melt electrowriting (MEW) in an optimized predictable way subsequently placed into a customized bioreactor system, which then filled with hydrogel precursor solution. The aqueous environment above lower critical solution temperature (LCST) PcycloPrOx at...
Atherosclerotic blood vessels overexpress connective tissue growth factor (CTGF) mRNA, but the role of CTGF in atherosclerosis remains controversial. To assess hypothesis that is involved atherosclerotic plaque progression, we investigated protein expression and distribution different types morphology.Serial cross-sections 45 human carotid plaques were immunohistochemically analyzed for presence protein, neovascularization (von Willebrand factor), macrophages (CD68), T cells (CD3). The...
Abstract Statins were shown to possess immunomodulating properties, but the mechanisms of statin effects on immune system are poorly understood. We analyzed influence statins professional antigen-presenting dendritic cells (DC). Immature DC cultivated from monocytes healthy donors. maturation was induced by lipopolysaccharide (LPS; 1 μg/mL). Unstimulated and LPS-stimulated treated with simvastatin or atorvastatin (0.1–1 μM). The expression CD40, CD83, CD86, human leukocyte antigen-DR...
The longevity of valved right ventricle to pulmonary artery (RV-PA) conduits is limited due calcification and degeneration non-viable structures. Xenografts are commonly used because the restricted availability cryopreserved homografts. Tissue-engineered (de-cellularised) valves (TEPVs) were thought be a valuable alternative homografts postoperative seeding with viable autologous vascular endothelial cells.From July 2007 December 2008, xenogenous TEPV (Matrix P plus) implanted in 16 patients...
Rupture of atherosclerotic plaques is one the main causes ischemic strokes. The aim this study was to investigate carotid plaque vulnerability markers in relation blood flow direction and mechanisms leading rupture at upstream side stenoses.Frequency location rupture, endothelial erosion, neovascularization, hemorrhage were determined longitudinal sections 80 human specimens. Plaques immunohistochemically analyzed for vulnerability. Plaque geometry measured reconstruct shape profiles...
Abstract: Signaling pathways induced by the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) play a key role in cellular responses to inflammation and injury. In cardiovascular system, TNF-α -activated signal transduction may contribute vascular dysfunction, development progression of atherosclerosis, adverse cardiac remodeling following myocardial infarction heart failure. This review addresses physiology disease. Furthermore, therapeutic benefits systemic antagonism autoimmune...
Novel hybrid hydrogels based on alginate and keratin were successfully produced for the first time. The self-assembly properties of keratin, its ability to mimic extracellular matrix combined with excellent chemical mechanical stability biocompatibility produce 2D 3D hydrogels. These prepared using two different approaches: sonication, obtain hydrogels, a pressure-driven extrusion technique All results indicated that composition had significant effect their physical properties, they can...
Flow cytometry for intracellular SPION quantification: specificity and sensitivity in comparison with spectroscopic methods Ralf P Friedrich,1 Christina Janko,1 Marina Poettler,1 Philipp Tripal,1 Jan Zaloga,1 Iwona Cicha,1 Stephan Dürr,1,2 Johannes Nowak,3 Stefan Odenbach,3 Ioana Slabu,4 Maik Liebl,4 Lutz Trahms,4 Marcus Stapf,5 Ingrid Hilger,5 Lyer,1 Christoph Alexiou1 1Department of Otorhinolaryngology, Head Neck Surgery, Section Experimental Oncology Nanomedicine, University...
We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static dynamic cell culture conditions.Size, polydispersity ζ-potential were determined 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric iron oxide nanoparticles. Nanoparticle effects on primary human endothelial viability monitored using real-time live-cell microscopy conditions, a flow model arterial bifurcations.The majority...
Apoptosis of polymorphonuclear neutrophils (PMN) is currently discussed as a key event in the control inflammation. This study determined PMN apoptosis and its underlying mechanisms controls (C), patients with stable (SAP) or unstable angina (UAP), acute myocardial infarction (AMI). Blood was drawn from 15 subjects each C, SAP, UAP, AMI. measured by flow cytometry isolated (propidium iodide staining) whole blood (CD16, FcgammaRIII). Serum cytokines were enzyme-linked immunosorbent assay....