A5025606174- Drug-Induced Adverse Reactions
- Food Allergy and Anaphylaxis Research
- Nanoparticle-Based Drug Delivery
- Complement system in diseases
- Neuroscience and Neuropharmacology Research
- Inhalation and Respiratory Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- EEG and Brain-Computer Interfaces
- Traumatic Brain Injury and Neurovascular Disturbances
- Biosimilars and Bioanalytical Methods
- Nanoparticles: synthesis and applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Asthma and respiratory diseases
- Neonatal and fetal brain pathology
- Lipid metabolism and disorders
- Advanced MRI Techniques and Applications
- Advanced Drug Delivery Systems
- Allergic Rhinitis and Sensitization
- Mitochondrial Function and Pathology
- Neurological Disease Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Metabolism and Genetic Disorders
- Neural dynamics and brain function
Semmelweis University
1989-2024
Veroscience (United States)
2015
University of Pennsylvania
1984-1989
Max Planck Institute of Molecular Physiology
1978
Polyethylene glycol (PEG)-coated nanopharmaceuticals can cause mild to severe hypersensitivity reactions (HSRs), which occasionally be life threatening or even lethal. The phenomenon represents an unsolved immune barrier the use of these drugs, yet its mechanism is poorly understood. This study showed that a single i.v. injection in pigs low dose PEGylated liposomes (Doxebo) induced massive rise anti-PEG IgM blood, peaking at days 7–9 and declining over 6 weeks. Bolus injections...
Abstract: Iron oxide-based contrast agents have been in clinical use for magnetic resonance imaging (MRI) of lymph nodes, liver, intestines, and the cardiovascular system. Superparamagnetic iron oxide nanoparticles (SPIONs) high potential as a agent MRI, but no intravenous oxide-containing are currently approved imaging. The aim our work was to analyze hemocompatibility immuno-safety new type dextran-coated SPIONs (SPIONdex) characterize these with ultra-high-field MRI. Key parameters...
We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static dynamic cell culture conditions.Size, polydispersity ζ-potential were determined 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric iron oxide nanoparticles. Nanoparticle effects on primary human endothelial viability monitored using real-time live-cell microscopy conditions, a flow model arterial bifurcations.The majority...
Intravenous administration of liposomal drugs can entail infusion reactions, also known as hypersensitivity reactions (HSRs), that be severe and sometimes life-threatening in a small portion patients. One empirical approach to prevent these consists lowering the speed extending time drug. However, different have levels reactogenicity, which means optimal protocol for each drug may differ should identified evaluated make treatment safe convenient possible. The goal present study was explore...
The effect of the ganglioside GM1 on recovery local cerebral glucose metabolism (lCMRgl), kinetics cerebrocortical electrical activity, blood flow and redox state as well histological changes following focal ischemia has been studied in cat. Ischemia was produced by occlusion left middle artery (MCA), (30 mg/kg) injected intravenously at 30 min after MCA or time release occlusion, 120 min. Another group animals were subjected to same ischemic insult, but without treatment, sham-operated...
Abstract Pigs provide a sensitive and quantitative animal model of complement (C) activation-related pseudoallergy (CARPA) caused by liposomes wide range nanoparticulate drugs or drug nanocarriers (nanomedicines). The tetrad symptoms (hemodynamic, hematological, laboratory skin changes) that arise within minutes after i.v. injection reactogenic nanomedicines (RNMs) are highly reproducible among different pigs but the presence, direction relative severity very with RNMs their administration...
Background: It has been proposed that many hypersensitivity reactions to nanopharmaceuticals represent complement (C)-activation-related pseudoallergy (CARPA), and pigs provide a sensitive animal model study the phenomenon. However, recent suggested pulmonary hypertension, pivotal symptom of porcine CARPA, is not mediated by C in cases polystyrene nanoparticle (PS-NP)-induced reactions. Goals: To characterize PS-NPs reexamine contribution CARPA their reactivity pigs. Study design: activation...
Pigs provide a sensitive and quantitative animal model of non-IgE-mediated(pseudoallergic) hypersensitivity reactions (HSRs) caused by liposomes many other nanoparticulate drugs or drug-carrier nanosystems (nanomedicines). The rapidly arising symptoms, including cardiopulmonary, hemodynamic, hematological, blood chemistry skin changes, resemble the clinical picture in man undergoing infusion toreactogenic nanoparticles. In addition to summarizing basic features pig CARPA model,...
Abstract Pigs provide a highly sensitive and quantitative in vivo model for complement (C) activation-related pseudoallergy (CARPA), hypersensitivity reaction caused by some state-of-art nanomedicines. In an effort to understand the mechanism of pigs’ unique sensitivity CARPA, this review focuses on pulmonary intravascular macrophages (PIMs), which are abundantly present lung pigs. These cells represent macrophage subpopulation whose qualities explain characteristic symptoms CARPA species,...
Liposomes formulated from the 1,3-diamidophospholipid Pad-PC-Pad are shear-responsive and thus promising nano-containers to specifically release a vasodilator at stenotic arteries. The recommended preclinical safety tests for therapeutic liposomes of nanometer size include in vitro assessment complement activation evaluation associated risk activation-related pseudo-allergy (CARPA) vivo. For this reason, we measured by formulations human porcine sera, along with nanopharmaceutical-mediated...
Abstract To explore the role of complement (C) activation in hypersensitivity reactions (HSRs) to some anticancer drugs, as well use C biomarkers (C
The effect of the ganglioside GM1 was studied in a focal cerebral ischemia model 30 cats consisting 2 hours middle artery occlusion followed by 4 recirculation. cerebrocortical electrical activity, extracellular potassium and microcirculation indicated NAD/NADH fluorescence were measured during as well recirculation core territory, while metabolic rate for glucose (ICMRgl) at end classified into either mildly or moderately severe stroke groups based on depression activity occluded side. Of...