A5015234723- Food Allergy and Anaphylaxis Research
- Drug-Induced Adverse Reactions
- Anesthesia and Pain Management
- Cardiac Arrest and Resuscitation
- Veterinary Pharmacology and Anesthesia
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Monoclonal and Polyclonal Antibodies Research
- Allergic Rhinitis and Sensitization
- Advanced biosensing and bioanalysis techniques
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Interference and Gene Delivery
- Poisoning and overdose treatments
- Inhalation and Respiratory Drug Delivery
- Complement system in diseases
- Anesthesia and Sedative Agents
- Respiratory Support and Mechanisms
- Trauma and Emergency Care Studies
- Nanoparticle-Based Drug Delivery
- Hemoglobin structure and function
- Nanoparticles: synthesis and applications
- Biosimilars and Bioanalytical Methods
- Extracellular vesicles in disease
- Advanced Drug Delivery Systems
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pediatric Pain Management Techniques
Defense & Veterans Center for Integrative Pain Management
2011-2024
Henry M. Jackson Foundation
2018-2024
Uniformed Services University of the Health Sciences
2008-2024
Walter Reed National Military Medical Center
2022
Semmelweis University
2008-2012
Bay Zoltán Foundation for Applied Research
2012
Gottsegen National Cardiovascular Center
2006-2007
Reports in the recent experimental literature have provided contradicting results different animal species regarding efficacy of IV lipid emulsion (ILE) reversal cardiovascular and central nervous system symptoms local anesthetic other lipophilic drug overdoses. In particular, ILE seemed to be effective rats, rabbits, dogs, humans, but not swine, for which it only failed reverse adverse effects anesthetics, animals also developed a generalized cutaneous mottling or dusky appearance...
Abstract Pigs provide a sensitive and quantitative animal model of complement (C) activation-related pseudoallergy (CARPA) caused by liposomes wide range nanoparticulate drugs or drug nanocarriers (nanomedicines). The tetrad symptoms (hemodynamic, hematological, laboratory skin changes) that arise within minutes after i.v. injection reactogenic nanomedicines (RNMs) are highly reproducible among different pigs but the presence, direction relative severity very with RNMs their administration...
The unbalanced coagulation of blood is a life-threatening event that requires accurate and timely treatment. We introduce user-friendly biomolecular platform based on modular RNA-DNA anticoagulant fibers programmed for reversible extracellular communication with thrombin subsequent control anticoagulation via "kill-switch" mechanism restores hemostasis. To demonstrate the potential this reconfigurable technology, we designed tested set carry different thrombin-binding aptamers. All are...
Pigs provide a sensitive and quantitative animal model of non-IgE-mediated(pseudoallergic) hypersensitivity reactions (HSRs) caused by liposomes many other nanoparticulate drugs or drug-carrier nanosystems (nanomedicines). The rapidly arising symptoms, including cardiopulmonary, hemodynamic, hematological, blood chemistry skin changes, resemble the clinical picture in man undergoing infusion toreactogenic nanoparticles. In addition to summarizing basic features pig CARPA model,...
A 30-year-old man developed unexplained rhabdomyolysis, persistently increased creatine kinase and severe debilitating muscle cramps. After a nondiagnostic neurologic evaluation, he was referred for biopsy, to include histology/histochemistry, myoglobinuria panel, caffeine halothane contracture test. Only the test positive, subsequent ryanodine receptor type 1 gene evaluation revealed mutation functionally causative malignant hyperthermia. His identical twin brother, who suffering from...
Postsurgical follow-up calls enable nurses to assess a patient's condition, provide tailored education, and improve the experience. Despite benefits, barriers phone-based assessments may include patient nonresponse lack of time due demanding clinical schedules. The purpose this trial was examine feasibility utility smartphone app, mCare, for assessing pain, pain impact, peripheral nerve block effects in patients.Pilot randomized control trial.Eligible patients at military treatment facility...
Lipid emulsion (20%) is advocated as a rescue drug for local anesthetic toxicity. No study has measured the impact of lipid therapy on postmortem serum levels.We anesthetized Yorkshire swine (n = 11) and standard monitors were placed. The received 1.5 mg/kg/min IV ropivacaine until death (asystole). Blood samples drawn before infusion (baseline) at 5-minute intervals during measurement blood gases free, bound, total concentrations via high-performance liquid chromatography. Five only, 6 plus...
Admixtures of propofol-ketamine, propofol-ketamine-fentanyl, and propofol-ketamine-remifentanil were subjected to various clinically relevant conditions study their chemical stability. A novel high-performance liquid chromatography-mass spectrometry method revealed no degradation any compound by incubation at 37°C, constant mixing, or table-top storage for 6- 24-hour time periods, except variable recovery both propofol fentanyl in the admixtures propofol-ketamine-fentanyl suggesting possible...
Acute respiratory distress syndrome (ARDS) is a widespread and often fatal clinical marked by the acute onset of pulmonary edema inflammatory-mediated disruptions in alveolar-capillary permeability resulting impaired gas exchange tissue oxygenation with subsequent failure that accounts for 10.4% all intensive care unit admissions worldwide boasts mortality rate 38.5%. The current treatment ARDS remains largely supportive. This because many challenges achieving stable sustainable animal model...
Introduction Hemorrhage is the leading cause of preventable death in trauma both military and civilian settings worldwide. Medical studies from Operation Enduring Freedom (OEF) Iraqi (OIF) informed change prehospital medicine by influencing widespread tourniquet distribution training on their use to stop life-threatening extremity hemorrhage. In setting, there has been a significant reduction due exsanguination since implementation tourniquets within Department Defense. However,...
Environmental heat stress represents a pervasive threat to warfighters, athletes, and occupational workers, impacting performance increasing the risk of injury. Exertional illness (EHI) is spectrum clinical disorders severity. While frequently predictable, EHI can occur unexpectedly may be followed by long-term comorbidities, including cardiovascular dysfunction exercise intolerance. The objective this study was assess genetic factors contributing EHI. Whole-exome sequencing performed in...
Targeted regional optimization (TRO), a partial resuscitative endovascular balloon occlusion of the aorta strategy, may mitigate distal ischemia and extend window effectiveness for this adjunct. An automated device allow greater control precise regulation flow past balloon, while being less resource-intensive. The objective study was to assess technical feasibility novel advanced controller (APOC) in achieving TRO at multiple pressures.Female swine (n = 48, 68.1 ± 0.7 kg) were randomized...
The purpose of this article is to evaluate a free-breathing pulse sequence quantify myocardial T1 changes in swine model tachycardia-induced heart failure.Yorkshire were implanted with pacemakers and ventricularly paced at 200 beats/min induce failure. Animals scanned twice 1.5-T MRI scanner, once baseline A T1-mapping was performed during tidal respiration before 5 minutes after the administration gadolinium-chelate contrast agent. values compared between failure scans. percentage fibrosis...
Intravenous injection of complement (C)‐activating drugs in pigs can cause immediate cardiac anaphylaxis associated with myocardial ischemia and relative bradycardia during severe hypotension. We tested whether this paradoxical could be mediated by adenosine. Pigs were injected i.v. adenosine or various C‐activating without pretreatment nonselective receptor (A) blocker theophylline, selective A1/A2 antagonists cyclopentyl‐xanthine (CPX) ZM24135, respectively. ECG hemodynamic parameters...