A5063693691- Glioma Diagnosis and Treatment
- Meningioma and schwannoma management
- Brain Metastases and Treatment
- Extracellular vesicles in disease
- Traumatic Brain Injury and Neurovascular Disturbances
- Lung Cancer Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Biomedical Ethics and Regulation
- Pituitary Gland Disorders and Treatments
- History of Medical Practice
- MicroRNA in disease regulation
- Ethics in Clinical Research
- Surgical Simulation and Training
- Head and Neck Surgical Oncology
- Palliative Care and End-of-Life Issues
- Immune cells in cancer
- Artificial Intelligence in Healthcare and Education
- Anatomy and Medical Technology
- RNA Interference and Gene Delivery
- Management of metastatic bone disease
- Cardiac, Anesthesia and Surgical Outcomes
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Neurosurgical Procedures and Complications
- Diversity and Career in Medicine
Medisch Centrum Haaglanden
2016-2025
Leiden University Medical Center
2016-2025
Harvard University
2010-2025
Massachusetts General Hospital
2016-2025
Oncode Institute
2024
Brigham and Women's Hospital
2016-2023
Harvard NeuroDiscovery Center
2019-2023
Leiden University
2018-2023
University Medical Center Utrecht
2011-2022
Parnassia Groep
2022
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Nano-sized extracelullar vesicles (EVs) released by various cell types play important roles in a plethora of (patho)physiological processes and are increasingly recognized as biomarkers for disease. In addition, engineered EV EV-inspired liposomes hold great potential drug delivery systems. Major technologies developed high-throughput analysis individual include nanoparticle tracking (NTA), tunable resistive pulse sensing (tRPS) high-resolution flow cytometry (hFC). Currently, there is need...
Glioblastoma multiforme (GBM) is the most common primary brain tumor and without exception lethal. GBMs modify immune system, which contributes to aggressive nature of disease. Particularly, cells monocytic lineage, including monocytes, macrophages microglia, are affected. We investigated influence GBM-derived extracellular vesicles (EVs) on phenotype cells. Proteomic profiling showed GBM EVs be enriched with proteins functioning in matrix interaction leukocyte migration. appeared skew...
Background Understanding the pathogenic role of extracellular vesicles (EVs) in disease and their potential diagnostic therapeutic utility is extremely reliant on in‐depth quantification, measurement identification EV sub‐populations. Quantification EVs has presented several challenges, predominantly due to small size such as exosomes availability various technologies measure nanosized particles, each technology having its own limitations. Materials Methods A standardized methodology...
Gliomas are primary, diffusely infiltrating brain tumors. Microglia innate immune cells in the CNS and make up a substantial portion of tumor mass. Glioma shape their microenvironment, communicating with reprogramming surrounding cells, resulting enhanced angiogenesis, suppression, remodeling extracellular matrix. communicate microglia, part by releasing vesicles (EVs). Mouse glioma stably expressing palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null...
Background: Cells secrete different types of membrane vesicles (MVs), which may act as important entities in normal human physiology and various pathological processes. The established methods for quantification MVs require purification or preanalytical handling samples with labeling moieties. Aim: authors' aim was to develop a method high-throughput, labeling-free nonpurified MVs. Materials & methods: Scanning ion occlusion sensing technology, relies on the detection particles upon their...
Abstract BACKGROUND Although survival statistics in patients with glioblastoma multiforme (GBM) are well-defined at the group level, predicting individual patient remains challenging because of significant variation within strata. OBJECTIVE To compare statistical and machine learning algorithms their ability to predict GBM deploy best performing model as an online calculator. METHODS Patients undergoing operation for a histopathologically confirmed were extracted from Surveillance...
Epileptogenic glioblastomas are thought to convey a favorable prognosis, either due early diagnosis or potential antitumor effects of antiepileptic drugs. We investigated the relationship between survival and epilepsy at presentation, diagnosis, drug therapy in glioblastoma patients. Multivariable Cox regression was applied data 647 consecutive patients diagnosed with de novo 2005 2013 order investigate association In addition, we quantified valproic acid (VPA) treatment. Epilepsy correlated...
Abstract Background Breast cancer (BC) brain metastases (BM) can have discordant hormonal or human epidermal growth factor receptor 2 (HER2) expression compared with corresponding primary tumors. This study aimed to describe incidence, predictors, and survival outcomes of receptors associated subtype switching in BM. Methods BCBM patients seen at 4 tertiary institutions who had undergone BM resection biopsy were included. Surgical pathology reports retrospectively assessed determine...
Glioblastomas are the most common and lethal primary brain tumors. Microglia, resident immune cells of brain, survey their environment respond to pathogens, toxins, Glioblastoma communicate with microglia, in part by releasing extracellular vesicles (EVs). Despite presence large numbers microglia glioblastoma, tumors continue grow, these neuroimmune appear incapable keeping tumor check. To understand this process, we analyzed gene expression interacting glioblastoma cells.We used RNASeq...
OBJECTIVE If not anticipated and prearranged, hospital stay can be prolonged while the patient awaits placement in a rehabilitation unit or skilled nursing facility following elective spine surgery. Preoperative prediction of likelihood postoperative discharge to any setting other than home (i.e., nonroutine discharge) after inpatient surgery would helpful terms decreasing length stay. The purpose this study was use machine learning algorithms develop an open-access web application for...