A5052463710- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Research and Splicing
- HIV Research and Treatment
- Reproductive System and Pregnancy
- Cytomegalovirus and herpesvirus research
- Cancer-related molecular mechanisms research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CRISPR and Genetic Engineering
- Mentoring and Academic Development
- Cancer, Hypoxia, and Metabolism
- Healthcare professionals’ stress and burnout
- Advanced Nanomaterials in Catalysis
- Health and Medical Research Impacts
- Innovation and Socioeconomic Development
Johns Hopkins University
2017-2024
University of Baltimore
2024
Johns Hopkins Medicine
2017-2023
La Trobe University
2022
German Cancer Research Center
2022
Heidelberg University
2022
Senescent cells (SnCs) are increasingly recognized as central effector in age-related pathologies. Extracellular vesicles (EVs) potential cellular communication tools through which SnCs exert functions the local tissue environment. To test this hypothesis a medical indication that could be validated clinically, we evaluated EV production from enriched chondrocytes isolated human arthritic cartilage. increased dose-responsive manner concentration of increased. The EVs were capable...
Brain tissue-derived extracellular vesicles (bdEVs) play neurodegenerative and protective roles, including in Alzheimer's disease (AD). Extracellular (EVs) may also leave the brain to betray state of CNS periphery. Only a few studies have profiled proteome bdEVs source tissue. Additionally, focusing on bdEV cell type-specific surface markers are rare.We aimed reveal pathological mechanisms inside by profiling tissue proteomes AD patients. In addition, indicate targets for capturing molecular...
We wished to re-assess the relative stability of microRNAs (miRNAs) as compared with other RNA molecules, which has been confirmed in many contexts. When bound Argonaute proteins, miRNAs are protected from degradation, even when released into extracellular space ribonucleoprotein complexes, and or without protection membranes vesicles. Purified also appear present less a target for degradation than RNAs. Although by no means immune biological samples subjected prolonged incubation at room...
Hypoxia, or low oxygen tension, is frequently found in highly proliferative solid tumors such as anaplastic thyroid carcinoma (ATC) and believed to promote resistance chemotherapy radiation. Identifying hypoxic cells for targeted therapy may thus be an effective approach treating aggressive cancers. Here, we explore the potential of well-known hypoxia-responsive microRNA (miRNA) miR-210-3p a cellular extracellular biological marker hypoxia. We compare miRNA expression across several ATC...
Abstract Problem All trainees, especially those from historically minoritized backgrounds, experience stresses that may reduce their continuation in science, technology, engineering, math, and medicine (STEMM) careers. The Johns Hopkins University School of Medicine is one ∼45 institutions with a National Institutes Health funded Postbaccalaureate Research Education Program (PREP) provides mentoring year fulltime research to prepare students excluded groups for graduate school. Having...
Variants of the apolipoprotein E (APOE) gene are greatest known risk factors for sporadic Alzheimer disease (AD). Three major APOE isoform alleles, ε2, ε3, and ε4, encode produce proteins that differ by only 1-2 amino acids but have different binding partner interactions. Whereas ε2 is protective against AD relative to ε4 associated with an increased development. However, role in regulation pathogenesis has remained largely undetermined. Extracellular vesicles (EVs) lipid bilayer-delimited...
CRISPR/Cas9 systems have been advanced as promising tools in the HIV eradication armamentarium for sequence-specific disruption or latency reversal. Enthusiasm is balanced by concerns about off-target host genome modification and effects on evolution. In chronically HIV-1-infected U1 promonocytic model, we confirmed stimulation of HIV-1 production a mutant Cas9-transcriptional activator guide RNAs with two apparently more potent than one. However, significant increases were also observed...
Cervicovaginal secretions, or their components collected, are referred to as cervicovaginal lavage (CVL). CVL constituents have utility biomarkers and play protective roles in wound healing against HIV-1 infection. However, several of fluids less well understood, such extracellular RNAs carriers, for example, vesicles (EVs). EVs comprise a wide array double-leaflet membrane particles range diameter from 30 nm over one micron. The aim this study was determine whether differentially regulated...
ABSTRACT Hypoxia, or low oxygen tension, is frequently found in highly proliferative solid tumors such as anaplastic thyroid carcinoma (ATC) and believed to promote resistance chemotherapy radiation. Identifying hypoxic cells for targeted therapy may thus be an effective approach treating aggressive cancers. Here, we explore the potential of well-known hypoxia-responsive microRNA (miRNA) miR-210-3p a cellular extracellular biological marker hypoxia. We compare miRNA expression across several...
ABSTRACT Thyroid cancer is the most prevalent endocrine malignancy. We and others have shown that several microRNAs, which are post-transcriptional gene regulators, aberrantly expressed in anaplastic thyroid (ATC) papillary (PTC) tissues, as well cell lines derived from these cancers. In cell, miRNAs bound to Argonaute (AGO) proteins what could be termed low molecular weight RNA-Induced Silencing Complexes (LMW-RISCs) can assemble with additional proteins, mRNA, translation machinery into...
Abstract Introduction: Hypoxia is frequent in solid tumors and stabilizes Inducible Factor-1 (HIF-1). HIF-1, turn, activates several cellular pathways that promote tumor growth resistance to chemotherapy. MicroRNA (miRNA) miR-210 a direct HIF-1 target. binds Hypoxia-Response Element (HRE) the promoter, up-regulating its expression. miR-21 most widely reported oncogenic miRNA cancer. miRNAs complexed with Argonaute-2 (AGO2) bind target mRNAs inhibit translation. Given single can have hundreds...
Abstract The relative stability of microRNAs (miRNAs) as compared with other RNA molecules has been confirmed in many contexts. When bound to Argonaute (AGO) proteins, miRNAs are protected from degradation, even when released into the extracellular space ribonucleoprotein complexes, and or without protection membranes vesicles (EVs). Purified also appear present less a target for degradation than RNAs. Although by no means immune biological samples subjected prolonged incubation at room...
Abstract The goal of this study was to characterize extracellular vesicles (EVs) and miRNAs primate cervicovaginal lavage (CVL) during the menstrual cycle simian immunodeficiency virus (SIV) infection, determine if differentially regulated CVL might influence retrovirus replication. peripheral blood were collected from SIV-infected uninfected macaques. EVs enriched by stepped ultracentrifugation characterized thoroughly. miRNA profiles assessed with a medium-throughput stem-loop/hydrolysis...
ABSTRACT Alzheimer’s disease (AD) is a public health crisis that grows as populations age. Hallmarks of this neurodegenerative include aggregation beta-amyloid peptides and hyperphosphorylated tau proteins in the brain. Variants APOE gene are greatest known risk factors for sporadic AD. As emerging players AD pathophysiology, extracellular vesicles (EVs) contain proteins, lipids, RNAs involved disposal cellular toxins intercellular communication. AD-related changes molecular composition EVs...