A5076543673- MicroRNA in disease regulation
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Hepatitis B Virus Studies
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Glioma Diagnosis and Treatment
- Cervical Cancer and HPV Research
- PI3K/AKT/mTOR signaling in cancer
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Extracellular vesicles in disease
- Nuclear Structure and Function
- MXene and MAX Phase Materials
- Cancer-related Molecular Pathways
- Gastrointestinal disorders and treatments
- Cancer Mechanisms and Therapy
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Gastric Cancer Management and Outcomes
Memorial Sloan Kettering Cancer Center
2015-2025
Kettering University
2017-2025
University of Palermo
2004-2025
University of Padua
2024
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2023
National Institute of General Medical Sciences
2021
Cancer Institute (WIA)
2021
National Cancer Institute
2021
National Institutes of Health
2021
Enzo Life Sciences (United States)
2020
Glutamine-based PET imaging takes advantage of gliomas’ glutamine addiction and can be used to assess metabolic nutrient uptake in gliomas.
A subset of childhood posterior fossa ependymomas with poor prognosis is epigenetically similar to H3K27M gliomas.
Significance MicroRNAs limit gene expression by recruiting a large protein complex known as the RNA-induced silencing (RISC) to target mRNAs. While attempting understand physiological regulation of RISC assembly, we found that most healthy adult tissues retain reserve microRNAs not stably associated with mRNA. Recruitment mRNA-containing complexes was accompanied an increase in their ability repress targets and regulated part phosphoinositide-3 kinase–RAC-alpha serine/threonine-protein...
Abstract MicroRNA 145 (miR145) has been proposed as a tumor suppressor. It was previously shown that miR145 targets the 3′ UTR of insulin receptor substrate‐1 (IRS‐1) and dramatically inhibits growth colon cancer cells. also type 1 insulin‐like factor (IGF‐IR). We show here an IRS‐1 lacking its is no longer down‐regulated by rescues cells from miR145‐induced inhibition growth. An IGF‐IR resistant to (again elimination UTR) not but fails rescue inhibition. These other results, taken together,...
ATP-dependent nucleosome-remodeling enzymes and covalent modifiers of chromatin set the functional state chromatin. However, how these enzymatic activities are coordinated in nucleus is largely unknown. We found that evolutionary conserved ATPase ISWI poly-ADP-ribose polymerase PARP genetically interact. present evidence showing target poly-ADP-ribosylation. Poly-ADP-ribosylation counteracts function vitro vivo. Our work suggests a physiological poly-ADP-ribosylation can be new, important...
Cellular proliferation depends on the integration of mitogenic stimuli with environmental conditions. Increasing evidence suggests that microRNAs play a regulatory role in this integration. Here we show during periods cellular quiescence, mature are stabilized and stored Argonaute protein complexes can be activated by stimulation to repress mitogen-stimulated targets, thus influencing subsequent responses. In quiescent cells, majority exist low molecular weight, protein-containing devoid...
Although virtually all gene networks are predicted to be controlled by miRNAs, the contribution of this important layer regulation tissue homeostasis in adult animals remains unclear. Gain and loss-of-function experiments have provided key insights into specific function individual but effective genetic tools study functional consequences global inhibition miRNA activity vivo lacking. Here we report generation characterization a genetically engineered mouse strain which miRNA-mediated...
Focal gene amplifications are among the most common cancer-associated mutations1 but have proven challenging to engineer in primary cells and model organisms. Here we describe a general strategy large (more than 1 Mbp) focal mediated by extrachromosomal DNAs (ecDNAs)2 spatiotemporally controlled manner mice. By coupling ecDNA formation with expression of selectable markers, track dynamics ecDNA-containing under physiological conditions presence specific selective pressures. We also apply...
Abstract Chronic lymphocytic leukemia (CLL) is the most common in world. The TCL1 gene, responsible for prolymphocytic T cell leukemia, also overexpressed human B malignancies and overexpression of Tcl1 protein occurs frequently CLL. Aging transgenic mice that overexpress under control μ immunoglobulin gene enhancer, develop a CD5+ lymphoproliferative disorder mimicking CLL implicating pathogenesis In current study, we exploited this mouse to investigate two different CLL-related issues:...
The bulk of RNA produced from the genome complex organisms consists a very large number transcripts lacking protein translational potential and collectively known as noncoding RNAs (ncRNAs). Initially thought to be mere products spurious transcriptional noise, ncRNAs are now universally recognized pivotal players in cell regulatory networks across broad spectrum biological processes. Owing their critical roles, ncRNA dysfunction is closely associated with etiopathogenesis various human...
ABSTRACT Focal gene amplifications are among the most common cancer-associated mutations, but their evolution and contribution to tumorigenesis have proven challenging recapitulate in primary cells model organisms. Here we describe a general approach engineer large (>1 Mbp) focal mediated by extrachromosomal circular DNAs (ecDNAs, also known as “double minutes”) spatiotemporally controlled manner cancer cell lines derived from genetically engineered mice. With this strategy, ecDNA...
Abstract Focal gene amplifications are among the most common cancer-associated mutations but have proven challenging to engineer in primary cells and model organisms. Here we describe a general strategy large (more than 1 Mbp) focal mediated by extrachromosomal DNAs (ecDNAs) spatiotemporally controlled manner mice. By coupling ecDNA formation with expression of selectable markers, track dynamics ecDNA-containing under physiological conditions presence specific selective pressures. We also...
Nucleosome remodeling and covalent modifications of histones play fundamental roles in chromatin structure function. However, much remains to be learned about how the action ATP-dependent factors histone-modifying enzymes is coordinated modulate organization transcription. The evolutionarily conserved chromatin-remodeling factor ISWI plays essential chromosome organization, DNA replication, transcription regulation. To gain insight into regulation mechanism ISWI, we conducted an unbiased...
CAM is used by about 40% of cancer patients in Western Countries, with peaks 80% for breast patients. Cancer use to boost immune function, control symptoms and treatment-related side effects, improve health-related quality life (HR-QoL) survival. Unfortunately, self-prescription natural remedies can lead unexpected toxicities reduce the effectiveness therapy. Although usually refers all "natural or organic" products/methods that are generally considered less toxic, there concerns drug...
Aging wine on lees results in the release of different yeast components, including peptides, whose role is unclear. In this study, peptides released a synthetic must, fermented with an oenological strain, and aged for 180 days were quantified (RP-HPLC) identified (LC-MS/MS) at time points. A rapid increase peptide concentration was observed first two months, over 2600 sequences identified. During following four remained constant, while their variety decreased slightly, probably due to...
Cell growth and proliferation require the coordinated activation of many cellular processes, including cap-dependent mRNA translation. MicroRNAs oppose translation set thresholds for expression target proteins. Emerging data suggest that microRNA function is enhanced by due in part to induction RNA-induced silencing complex (RISC) scaffold protein GW182. In current study, we demonstrate increased GW182 activated or transformed immune cells results from effects phosphoinositol...
We have examined several microRNAs (miRs) indicated by the databases as targeting signaling pathway of type-1 insulin-like growth factor receptor (IGF-IR). Most miRs tested had multiple targets, expected, leading to cell death. However, miR145 seemed affect its tumor suppressor activity largely downregulating docking protein IGF-IR, insulin substrate-1 (IRS-1). These results suggest that, despite many targets provided databases, in some cases a single target can be predominant determining...