A5101610684- Single-cell and spatial transcriptomics
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Colorectal Cancer Screening and Detection
- Cancer Cells and Metastasis
- Adipokines, Inflammation, and Metabolic Diseases
- Traditional Chinese Medicine Studies
- Acupuncture Treatment Research Studies
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Diverticular Disease and Complications
- Alcohol Consumption and Health Effects
- Bacteriophages and microbial interactions
- Liver Disease Diagnosis and Treatment
- Fungal and yeast genetics research
- CAR-T cell therapy research
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Nutrition and Health in Aging
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
- Vagus Nerve Stimulation Research
- Cancer Risks and Factors
Vanderbilt University
2022-2025
Harbin Medical University
2022-2025
Case Western Reserve University
2014-2024
Vanderbilt University Medical Center
2022-2024
University School
2024
University Hospitals of Cleveland
2018-2024
First Affiliated Hospital of Xiamen University
2024
University of Wisconsin–Madison
2022-2023
Zoucheng People's Hospital
2022
Center for Environmental Health
2018
Colorectal cancer exhibits dynamic cellular and genetic heterogeneity during progression from precursor lesions toward malignancy. Analysis of spatial multi-omic data 31 human colorectal specimens enabled phylogeographic mapping tumor evolution that revealed individualized trajectories accompanying microenvironmental clonal alterations. Phylogeographic ordered events, classified tumors by their evolutionary dynamics, placed regions along global pseudotemporal encompassing the chromosomal...
Summary: Research on precancers, as defined at-risk tissues and early lesions, is of high significance given the effectiveness intervention. We discuss need for risk stratification to prevent overtreatment, an emphasis role genetic epigenetic aging when considering risk, importance integrating macroenvironmental factors with molecules cells in lesions normal developing effective intervention health policy strategies.
Temporal ordering of cellular events offers fundamental insights into biological phenomena. Although this is traditionally achieved through continuous direct observations
Abstract Background Chronic inflammation is a risk factor for colorectal cancer (CRC). Circulating C-reactive protein (CRP) also moderately associated with CRC risk. However, observational studies are susceptible to unmeasured confounding or reverse causality. Using genetic variants as instrumental variables, we investigated the causal relationship between genetically elevated CRP concentration and risk, using Mendelian randomization approach. Methods Individual-level data from 30 480 cases...
Aerobic glycolysis plays a key role in cancer cell metabolism and contributes to tumorigenesis, including that of non-small lung (NSCLC). Tanshinone IIA (Tan IIA), an active compound Salvia miltiorrhiza, exhibits antitumor properties. Multiple mechanisms are involved the action Tan cancer, such as inhibiting growth, promoting apoptosis influencing cellular metabolism. However, effects on NSCLC cells its remain unclear. The present study shows dose-dependently attenuated growth vitro...
DNA is a universal and programmable signal of living organisms. Here we develop cell-based sensors by engineering the naturally competent bacterium Bacillus subtilis (B. subtilis) to detect specific sequences in environment. The sensor strains can identify diverse bacterial species including major human pathogens with high specificity. Multiplexed detection genomic from different complex samples be achieved coupling sensing mechanism orthogonal fluorescent reporters. We also demonstrate that...
Journal Article Structure of the human gene for monoamine oxidase type A Get access Zheng-Yi Chen, Chen Search other works by this author on: Oxford Academic PubMed Google Scholar Gokhan S. Hotamisligil, Hotamisligil 1Neuroscience Center (Neurology), Massachusetts General Hospital-EastBuilding 149, Charlestown, MA 02129, USA2Department Microbiology and Molecular Genetics, Harvard Medical SchoolBoston, 02115, USA Jenq-Kuen Huang, Huang 3Department Chemistry, Western Illinois UniversityMacomb,...
The tumor microenvironment plays a key role in the pathogenesis of colorectal tumors and contains various cell types including epithelial, immune, mesenchymal cells. Characterization interactions between these is necessary for revealing complex nature tumors. In this study, we used single-cell RNA-seq (scRNA-seq) to compare microenvironments mouse model sporadic adenoma (Lrig1 CreERT2/+ ;Apc 2lox14/+ ) inflammation-driven cancer induced by azoxymethane dextran sodium sulfate (AOM/DSS). While...
Although selenium deficiency correlates with colorectal cancer (CRC) risk, the roles of selenium-rich antioxidant selenoprotein P (SELENOP) in CRC remain unclear. In this study, we defined SELENOP's contributions to sporadic CRC. human single-cell cRNA-Seq (scRNA-Seq) data sets, discovered that SELENOP expression rose as normal colon stem cells transformed into adenomas progressed carcinomas. We next examined effects Selenop KO a mouse adenoma model involved conditional, intestinal...
Droplet-based single-cell RNA-seq (scRNA-seq) data are plagued by ambient contaminations caused nucleic acid material released dead and dying cells. This is mixed into the buffer co-encapsulated with cells, leading to a lower signal-to-noise ratio. Although there exist computational methods remove post-hoc, reliability of algorithms in generating high-quality from low-quality sources remains uncertain. Here, we assess quality before filtering set quantitative, contamination-based metrics...
Abstract Background Evobrutinib, a third-generation Bruton's tyrosine kinase (BTK) inhibitor, shows great promise for treating neuroinflammatory diseases due to its small molecular size, ease of absorption, and ability cross the blood–brain barrier. Although previous studies have confirmed significant BTK expression in microglia, potential Evobrutinib treat ischemic stroke by modulating microglial function underlying mechanisms remain be elucidated. Methods Male C57BL/6 mice with cerebral...
Abstract An active immune system plays a central role in preventing the progression from precancerous lesions to invasive cancers. Identifying molecular markers that dictate effective surveillance is essential for advancing cancer prevention strategies. A higher level of cell infiltration observed serrated lesions, subtype colorectal premalignant comparison other common subtype, conventional adenoma. However, mechanism underlying elevated response remains poorly understood because...
It has been reported that the nervous system can regulate immune reactions through various mechanisms. However, role of splenic sympathetic nerve activity in autoimmune during pathogenesis experimental encephalomyelitis (EAE) remained unclear. Here, we blocked and found number adaptive cells, such as CD4+ T CD8+ cells B were upregulated. Additionally, there was an increase secretion inflammatory cytokines spleen, neurological symptoms EAE exacerbated. In vitro experiments, norepinephrine...
During menopause, up to 80% of individuals experience vasomotor symptoms, such as hot flashes, that severely impact quality life. Fezolinetant is a novel oral drug shows promise in treating flashes by acting neurokinin 3 receptor antagonist restores hypothalamic thermoregulation. This review explores the effectiveness reducing menopausal symptoms compared current pharmacological standard care. We evaluate strengths an alternative treatment for patients are eligible traditional hormone...
BACKGROUND Multitarget stool DNA (mt‐sDNA) is an approved method for colon cancer screening that especially relevant patients who cannot undergo colonoscopy. Although the test performance has been evaluated in a large clinical trial, it was limited to predominantly white population. Given differences epidemiology and biology of African American individuals, authors sought compare mt‐sDNA between racial groups. METHODS The prospectively identified aged ≥40 years were referred colonoscopy at...
The zebrafish mechanosensory lateral line (LL) is a model system for the study of hair cell development, survival and regeneration. Recently, histone modifications have attracted considerable amount interest because their indispensable roles in various kinds cellular processes including differentiation, proliferation, apoptosis function. Lysine specific demethylase 1 (LSD1) an important enzyme that regulates methylation. As transcriptional regulator, this has broad functional...
Abstract Tissue profiling technologies present opportunities for understanding transition from precancerous lesions to malignancy, which may impact risk stratification, prevention, and even cancer treatment. A human precancer atlas building effort is ongoing tackle the significant challenge of decoding heterogeneity among cells, specimens, patients. Here, we discuss findings resulting atlases built across types, including those found in colon, breast, lung, stomach, cervix, skin, using bulk,...
Journal Article An X chromosome inactivation assay based on differential methylation of a CpG island coupled to VNTR polymorphism at the 5′ end monoamine oxidase A gene Get access Rudolf W. Hendriks, Hendriks 1Genetics Laboratory, Department Biochemistry, University OxfordSouth Parks Road, Oxford OX1 3QU, UK2Division lmmunobiology and Genetics, Immunohaematology, Medical CentreLeiden, The Netherlands Search for other works by this author on: Academic PubMed Google Scholar Zheng-Yi Chen, Chen...
Undifferentiated intestinal stem cells (ISCs) are crucial for maintaining homeostasis and resolving injury. Lgr5+ in the crypt base constantly divide, pushing daughter upward along axis where they differentiate into specialized cell types. Coordinated execution of complex transcriptional programs is necessary to allow maintenance undifferentiated while permitting differentiation wide array homeostasis. Previously, members myeloid translocation gene (MTG) family have been identified as...