A5087064238- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Cell Image Analysis Techniques
- RNA modifications and cancer
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Viral Infectious Diseases and Gene Expression in Insects
- Gene expression and cancer classification
- Cardiac Fibrosis and Remodeling
- Connexins and lens biology
- Molecular Biology Techniques and Applications
- 14-3-3 protein interactions
German Cancer Research Center
2015-2024
Heidelberg University
2014-2024
Deutschen Konsortium für Translationale Krebsforschung
2024
University Hospital Heidelberg
2012-2024
La Trobe University
2022
Johns Hopkins University
2022
BioClinicum (Russia)
2019
Ruhr University Bochum
2008-2013
National Center for Tumor Diseases
2012
University Medical Center Hamburg-Eppendorf
2012
MicroRNAs (miRNAs), a class of post-transcriptional gene expression regulators, have recently been detected in human body fluids, including peripheral blood plasma as extracellular nuclease resistant entities. However, the origin and function circulating miRNA remain essentially unknown. Here, we confirmed that mature contrast to mRNA or snRNA is strikingly stable cell culture media. Furthermore, found most media completely passed through 0.22 µm filters but remained supernatant after...
Nuclease resistant extracellular miRNAs have been found in all known biological fluids. The function of remains questionable; however, strong evidence suggests that these can be more than just byproducts cellular activity. Some miRNA species might carry cell-cell signaling during various physiological and pathological processes. In this review, we discuss the state-of-the-art field intercellular transport highlight current theories regarding origin miRNAs.
In recent years, circulating miRNAs have attracted a great deal of attention as promising novel markers for various diseases. Here, we investigated their potential to serve minimally invasive, early detection breast cancer in blood plasma. We profiled extracted from the plasma stage patients (taken at time-point diagnosis) and healthy control individuals using TaqMan low-density arrays (TLDA). Selected candidates identified initial screen were further validated an extended study cohort 207...
The use of circulating tumor cells (CTC) as a prognostic marker in metastatic breast cancer (MBC) has been well established. However, their efficacy and accuracy are still under scrutiny mainly because methods enrichment identification. We hypothesized that miRNAs can predict the CTC status patients with MBC, tested for same. Furthermore, we aimed at establishing panel capable differentiating MBC cases from healthy controls.Circulating plasma CTC-positive CTC-negative controls, were profiled...
Studies of miRNA association with Argonaute (AGO) proteins in mammalian cells have indicated lack bias toward particular AGO. However, to our knowledge, the use quantitative methods for studying different AGOs has not been reported so far. In this work we compared total content AGO1 and AGO2 immunoprecipitates obtained from MCF7 adenocarcinoma using TaqMan Low Density Arrays successfully verified selected miRNAs qPCR. For most species profiles were well correlated, however, some demonstrated...
Over the last few years, circulating microRNAs (miRNAs) have emerged as promising novel and minimally invasive markers for various diseases, including cancer. We already showed that certain miRNAs are deregulated in plasma of breast cancer patients when compared to healthy women. Herein we further explored their potential serve early detection blood plasma. Circulating miR-127-3p, miR-376a miR-652, selected candidates from a miRNA array-based screening, were found be associated with first...
Development of novel approaches for regulating the expression angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease (TMPRSS2) is becoming increasingly important within context ongoing COVID-19 pandemic since these enzymes play a crucial role in cell infection. In this work we searched putative ACE2 TMPRSS2 regulation networks mediated by various miRNA isoforms (isomiR) across different human organs using publicly available paired miRNA/mRNA-sequencing data from The Cancer...
ABSTRACT Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processes by shuttling material out between cells. Tissue EVs may thus lend insights into disease mechanisms also betray when released easily accessed biological fluids. Since brain‐derived (bdEVs) their cargo serve as biomarkers neurodegenerative diseases, we evaluated modifications to published, rigorous protocol for separation from brain tissue studied effects processing variables on...
Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, trace amounts DNA or RNA still remains a major challenge, especially for degraded nucleic acids like circulating DNA. This together with high cost and time requirements impedes many important applications MPS in medicine fundamental science. We established fast, cheap highly efficient protocol called 'Capture Amplification by Tailing Switching' (CATS) to...
Extracellular vesicles (EVs) are released from different cell types in the central nervous system (CNS) and play roles regulating physiological pathological functions. Although brain-derived EVs (bdEVs) have been successfully collected brain tissue, there is not yet a "bdEV Atlas" of regions. To address this gap, we separated eight anatomical regions single individual subsequently characterized them by count, size, morphology, protein RNA content. The greatest particle yield was cerebellum,...
MicroRNAs (miRNAs) are 19-24 nt single-stranded RNAs which regulate gene expression by sequence-specific targeting of corresponding mRNAs. Extracellular miRNAs have been consistently detected in all human body fluids, and were shown to be prominent non-invasive biomarkers for various diseases including cancer. Albeit biological function cell-free miRNA remains questionable, some studies demonstrated that exosomes encapsulated extracellular could mediate inter-cellular signaling. While others...
Connexin43 (Cx43) is the most abundant gap junction protein of brain, where it predominantly expressed in astrocytes. Recent studies imply a role Cx43 regulation important cellular processes, including migration, proliferation, and shape formation. These processes are assumed to be reflected by proteome expressing cells. To analyze influence on astrocytic proteome, we used RNA interference downregulate expression this connexin cultures mouse We applied difference gel electrophoresis (DIGE)...
EDITORIAL article Front. Genet., 12 February 2014Sec. RNA Volume 5 - 2014 | https://doi.org/10.3389/fgene.2014.00030
Abstract MicroRNAs (miRNAs) are a family of short noncoding RNAs that posttranscriptionally regulate gene expression and play an important role in multiple cellular processes. A significant percentage miRNAs intragenic, which is often functionally related to their host genes playing either antagonistic or synergistic roles. In this study, we constructed analyzed the entire network intergenic interactions induced by intragenic miRNAs. We further focused on core network, was defined as union...
People living with HIV (PLH) have significantly higher rates of cognitive impairment (CI) and major depressive disorder (MDD) versus the general population. The enzyme neutral sphingomyelinase 2 (nSMase2) is involved in biogenesis ceramide extracellular vesicles (EVs), both which are dysregulated PLH, CI, MDD. Here we evaluated EcoHIV-infected mice for behavioral abnormalities relevant to depression cognition deficits, assessed biochemical effects nSMase2 inhibition. Mice were infected...