A5005947741- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Melanoma and MAPK Pathways
- Prion Diseases and Protein Misfolding
- Alzheimer's disease research and treatments
- Cancer Mechanisms and Therapy
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- interferon and immune responses
- Trace Elements in Health
- RNA Research and Splicing
- Synthesis and biological activity
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Ferroptosis and cancer prognosis
- Parkinson's Disease Mechanisms and Treatments
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Angiogenesis and VEGF in Cancer
- Salivary Gland Disorders and Functions
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
Florey Institute of Neuroscience and Mental Health
2016-2025
The University of Melbourne
2016-2025
The Royal Melbourne Hospital
2019-2024
Stem Cells Australia
2023
Austin Health
2012-2017
Olivia Newton-John Cancer Wellness & Research Centre
2013-2017
Ludwig Cancer Research
2014-2015
Ludwig Cancer Research
2011-2014
La Trobe University
2014
GlaxoSmithKline (Belgium)
2014
Abstract Prion diseases are fatal, transmissible neurodegenerative disorders associated with conversion of the host‐encoded prion protein (PrP C ) into an abnormal pathogenic isoform Sc ). Following exposure to infectious agent in acquired disease, infection is propagated lymphoid tissues prior neuroinvasion and spread within central nervous system. The mechanism dissemination perplexing due lack plausible PrP ‐containing mobile cells that could account for between infected uninfected...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Extracellular vesicles, including exosomes, are released by all cells, those of the nervous system. Capable delivering lipid, protein and nucleic acids to both nearby distal exosomes have been hypothesized play a role in progression many diseases To date, most analyses on these vesicles healthy diseased state relied studying from vitro sources, such as conditioned cell culture media, or body fluids. Here we taken critical approach enrichment characterization human frontal cortex. This method...
Lipid dyshomeostasis is associated with the most common form of dementia, Alzheimer's disease (AD). Substantial progress has been made in identifying positron emission tomography and cerebrospinal fluid biomarkers for AD, but they have limited use as front-line diagnostic tools. Extracellular vesicles (EVs) are released by all cells contain a subset their parental cell composition, including lipids. EVs from brain into periphery, providing potential source tissue specific lipid biomarkers....
Alzheimer's disease (AD) is the most common form of dementia and associated with deposition 39- to 43-amino acid beta-amyloid peptide (Abeta) in brain. C-terminal fragments (CTFs) amyloid precursor protein (APP) can accumulate endosomally derived multivesicular bodies (MVBs). These intracellular structures contain intraluminal vesicles that are released from cell as exosomes when MVB fuses plasma membrane. Here we have investigated role processing APP show these APP-CTFs, well Abeta. In...
Combination therapy with BRAF and MEK inhibition is currently in clinical development for the treatment of BRAF-mutated malignant melanoma. inhibitors are associated enhanced antigen-specific T-lymphocyte recognition vivo. Consequently, has been proposed as proimmunogenic there considerable enthusiasm combining immunotherapy. inhibit ERK phosphorylation regardless mutational status have reported to impair modulate dendritic cell function. In this study, we investigate effects on isolated T...
// Aparna Jayachandran 1 , 2 Matthew Anaka Prashanth Prithviraj Christopher Hudson Sonja J McKeown 3 Pu-Han Lo Laura Vella Colin R Goding 4 Jonathan Cebon Andreas Behren Ludwig Institute for Cancer Research, Melbourne-Austin Branch, Immunobiology Laboratory, Heidelberg, VIC 3084, Australia. Department of Medicine, University Melbourne, Victoria, 3010, Australia Anatomy and Neuroscience, Oxford, OX3 7DQ, UK Correspondence to: Behren, e-mail: andreas.behren@ludwig.edu.au Keywords:...
ABSTRACT Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processes by shuttling material out between cells. Tissue EVs may thus lend insights into disease mechanisms also betray when released easily accessed biological fluids. Since brain‐derived (bdEVs) their cargo serve as biomarkers neurodegenerative diseases, we evaluated modifications to published, rigorous protocol for separation from brain tissue studied effects processing variables on...
Alzheimer's disease is a progressive neurodegenerative disorder, with the strongest disease-associated changes observed at clinical or end-stage disease. Transcriptomic deregulation of miRNA expression can spread via "horizontal" RNA transfer through extracellular vesicles (EVs) to act in conjunction proteins, leading mRNA, which provide early signals indicate forthcoming neuropathological brain. Here, we analysed small content, particular, miRNA, contained brain-derived EVs isolated from...
TDP-43 proteinopathies are characterized by loss of nuclear expression and formation C-terminal fragmentation accumulation in the cytoplasm. Recent studies have shown that can accumulate RNA stress granules (SGs) response to cell stresses this could be associated with subsequent ubiquinated protein aggregates. However, initial mechanisms controlling endogenous SGs during chronic disease not understood. In study we investigated mechanism processing SH-SY5Y neuronal-like cells exposed...
Treatment of BRAF mutant melanoma with kinase inhibitors has been associated rapid tumor regression; however, this clinical benefit is short-lived, and most patients relapse. A number studies suggest that the extracellular environment promotes inhibitor resistance progression. Extracellular vesicles, such as exosomes, are functional mediators in environment. They small vesicles known to carry a concentrated group cargo serve intercellular communicators not only locally but also systemically....
We established a range of melanoma cell lines from patient material (Table 1) termed Ludwig-Melbourne-Melanoma (LM-MEL-) followed by unique number (method: Anaka et al., 2012). These have been human leucocyte antigen (HLA)-typed, their mutational status for common mutated genes in determined using the Sequenom MelCarta panel, and transcriptomes profiled. All are tested mycoplasma, ethical approval research purposes has granted Austin Health Human Research Ethics Committee (HREC). Therapeutic...
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease characterized by the deposition of misfolded proteins in motor cortex and neurons. Although multitude ALS-associated mutated have been identified, several linked to small extracellular vesicles such as exosomes involved cell−cell communication. This study aims determine proteome isolated from ALS subjects identify novel deregulated proteins. Motor (MCEVs) were human postmortem (n = 10) neurological control (NC, n 5) brain...
Parkinson's disease (PD) is an increasingly common neurodegenerative disease. It has been suggested that the etiology of idiopathic PD complex and multifactorial involving environmental contributions, such as viral or bacterial infections microbial dysbiosis, in genetically predisposed individuals. With advances our understanding gut-brain axis, there increasing evidence intestinal microbiota mammalian immune system functionally interact. Recent findings suggest a shift gut microbiome to...