A5054610818- Cellular transport and secretion
- Cutaneous Melanoma Detection and Management
- Nonmelanoma Skin Cancer Studies
- Bacteriophages and microbial interactions
- Cutaneous lymphoproliferative disorders research
- Viral gastroenteritis research and epidemiology
- Genetics, Aging, and Longevity in Model Organisms
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Fungal Infections and Studies
- Lipid Membrane Structure and Behavior
- Dermatologic Treatments and Research
- SARS-CoV-2 and COVID-19 Research
- Cancer and Skin Lesions
- Autophagy in Disease and Therapy
- Listeria monocytogenes in Food Safety
- Bacterial Genetics and Biotechnology
- COVID-19 Clinical Research Studies
- Escherichia coli research studies
- Microbial Inactivation Methods
- Parasitic Infections and Diagnostics
- Bacillus and Francisella bacterial research
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
Universität Hamburg
2024-2025
University Medical Center Hamburg-Eppendorf
2024-2025
Institut Pasteur
2015-2024
Klinikum Bremerhaven-Reinkenheide
2014-2024
Unidades Centrales Científico-Técnicas
2024
Instituto de Salud Carlos III
2022-2024
Centro Nacional de Microbiologia
2023-2024
Hochschule Bremerhaven
2023
Centro Nacional de Biotecnología
2021-2023
Universidad Autónoma de Madrid
2022-2023
In sexual reproduction of most animals, the spermatozoon provides DNA and centrioles, together with some cytoplasm organelles, to oocyte that is being fertilized. Paternal mitochondria their genomes are generally eliminated in embryo by an unknown degradation mechanism. We show that, upon fertilization, a Caenorhabditis elegans triggers recruitment autophagosomes within minutes subsequent paternal degradation. Whereas nematode-specific sperm membranous organelles ubiquitinated before...
Shigella bacteria invade macrophages and epithelial cells following internalization lyse the phagosome escape to cytoplasm. Galectin-3, an abundant protein in cells, belongs a family of beta-galactoside-binding proteins, galectins, with many proposed functions immune response, development, differentiation, cancer infection. Galectins are synthesized as cytosolic proteins non-classical secretion bind extracellular beta-galactosides. Here we analysed localization galectin-3 entry into cytosol...
In many cells endosomal vacuoles show clathrin coats of which the function is unknown. Herein, we that this coat predominantly present on early endosomes and has a characteristic bilayered appearance in electron microscope. By immunoelectron microscopy contains heavy as well light chain, but lacks adaptor complexes AP1, AP2, AP3, by it differs from endocytic vesicles recycling endosomes. The insensitive to short incubations with brefeldin A, disappears presence phosphatidylinositol 3-kinase...
Cell stress and infection promote the formation of ubiquitinated aggregates in both non-immune immune cells. These structures are recognised by autophagy receptor p62/sequestosome 1 substrates for selective autophagy. The intracellular growth Salmonella enterica occurs a membranous compartment, Salmonella-containing vacuole (SCV), is dependent on effectors translocated to host cytoplasm pathogenicity island-2 (SPI-2) encoded type III secretion system (T3SS). Here, we show that bacterial...
How the vesicular traffic of signaling molecules contributes to T cell receptor (TCR) signal transduction at immunological synapse remains poorly understood. In this study, we show that protein tyrosine kinase Lck, TCRζ subunit, and adapter LAT through distinct exocytic compartments, which are released in a differentially regulated manner. Lck release depends on MAL protein. Synaptic turn, conditions calcium- synaptotagmin-7-dependent fusion containing vesicles. Fusion vesicles synaptic...
Hearing relies on rapid, temporally precise, and sustained neurotransmitter release at the ribbon synapses of sensory cells, inner hair cells (IHCs). This process requires otoferlin, a six C
The enteropathogenic bacterium Shigella flexneri uses a type 3 secretion apparatus (T3SA) to transfer proteins dubbed translocators and effectors inside host cells, inducing bacterial uptake subsequent lysis of the entry vacuole. Once in cytoplasm, outer membrane protein IcsA induces actin polymerization, enabling cytoplasmic movement cell-to-cell spread bacteria. During this infectious process, S. is targeted by ATG8/LC3. effector IcsB was proposed inhibit LC3 recruitment masking region...
Transport of neo-synthesized influenza A virus (IAV) viral ribonucleoproteins (vRNPs) from the nucleus to plasma membrane involves Rab 11 but precise mechanism remains poorly understood. We used metal-tagging and immunolabeling visualize proteins cellular endomembrane markers by electron microscopy IAV-infected cells. Unexpectedly, we provide evidence that vRNP components Rab11 protein are present at a modified, tubulated endoplasmic reticulum (ER) extends all throughout cell, on irregularly...
Hepatocyte growth factor regulated tyrosine kinase substrate (Hrs), a main component of the 'bilayered' clathrin coat on sorting endosomes, was originally identified as activated receptors. We have analysed Hrs phosphorylation in response to epidermal (EGF) stimulation and show that evolutionary conserved tyrosines Y329 Y334 provide principal sites. is proposed concentrate ubiquitinated receptors within clathrin-coated regions via direct interaction with its UIM (ubiquitin motif) domain....
Summary Recent progress in biology and microscopy has made it possible to acquire multidimensional data on rapid cellular activities. Unfortunately, the analysis needed describe observed biological process still remains a major bottleneck. We here present novel method of studying membrane trafficking by monitoring vesicular structures moving along three‐dimensional cytoskeleton network. It allows dynamics such be qualitatively quantitatively investigated. Our tracking uses kymogram extract...
Microbial pathogens have evolved mechanisms to overcome immune responses and successfully infect their host. Here, we studied how Listeria monocytogenes evades detection by peptidoglycan (PGN) modification. By analyzing L. muropeptides, detected O-acetylated muramic acid residues. We identified an O-acetyltransferase gene, oatA, in the genome sequence. Comparison of PGN from parental isogenic oatA mutant strains showed that OatA O-acetylates PGN. also found O-acetylation confers resistance...
Bacterial pathogens recruit clathrin upon interaction with host surface receptors during infection. Here, using three different infection models, we observed that host–pathogen interactions induce tyrosine phosphorylation of heavy chain. This modification was critical for recruitment actin at bacteria–host adhesion sites bacterial internalization or pedestal formation. At the interface, assembled to form coated pits conventional size. Because such structures cannot internalize large...
ABSTRACT The genes encoding the ribonucleases RNase J1 and Y have long been considered essential for Bacillus subtilis cell viability, even before there was concrete knowledge of their function as two most important enzymes RNA turnover in this organism. Here we show that characterization is incorrect Δ rnjA rny mutants are both viable. As expected, strains grow relatively slowly, with doubling times hour range rich medium. Knockout major defects sporulation competence development programs....
Microsporidia are fungi-related intracellular pathogens that may infect virtually all animals, but poorly understood. The nematode Caenorhabditis elegans has recently become a model host for studying microsporidia through the identification of its natural microsporidian pathogen Nematocida parisii. However, it was unclear how widespread and diverse infections in C. or other related nematodes wild. Here we describe isolation culture 47 with infections. N. parisii is found to be most common...
Similar to many eukaryotic viruses (and unlike bacteriophages), infecting archaea are often encased in lipid-containing envelopes. However, the mechanisms of their morphogenesis and egress remain unexplored. Here, we used dual-axis electron tomography (ET) characterize Sulfolobus spindle-shaped virus 1 (SSV1), prototype family Fuselloviridae representative most abundant archaea-specific group viruses. Our results show that SSV1 assembly concomitant occur at cellular cytoplasmic membrane via...
Significance Shigella spp . are responsible for devastating diarrheal diseases, primarily in children, within underdeveloped countries. invades the mucosa of large intestine, causing inflammation and damage to epithelium. Here, we have measured progression infection a small animal model disease better understand mechanism invasion colonic mucosa. The novelty our approach relies on tracking fluorescent bacteria inside infected tissue at various time points using confocal microscopy subsequent...