A5011492082- Advanced Electron Microscopy Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Electron and X-Ray Spectroscopy Techniques
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Force Microscopy Techniques and Applications
- Escherichia coli research studies
- Listeria monocytogenes in Food Safety
- Cell Image Analysis Techniques
- Genomics and Phylogenetic Studies
- Lipid Membrane Structure and Behavior
- Photoacoustic and Ultrasonic Imaging
- ATP Synthase and ATPases Research
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Microfluidic and Capillary Electrophoresis Applications
- Material Dynamics and Properties
- Theoretical and Computational Physics
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Phytoplasmas and Hemiptera pathogens
Institut Pasteur
2010-2024
Max Planck Institute of Biochemistry
2005-2007
Max Planck Society
2005-2007
Institut Curie
2002-2003
Imperial College London
1983-1999
Abstract The orchestration of intercellular communication is essential for multicellular organisms. One mechanism by which cells communicate through long, actin-rich membranous protrusions called tunneling nanotubes (TNTs), allow the transport various cargoes, between cytoplasm distant in vitro and vivo. With most studies failing to establish their structural identity examine whether they are truly open-ended organelles, there a need study anatomy TNTs at nanometer resolution. Here, we use...
Mitochondria are essential and highly dynamic organelles, constantly undergoing fusion fission. We analyzed mitochondrial dynamics during infection with the human bacterial pathogen Listeria monocytogenes show that this profoundly alters by causing transient network fragmentation. Mitochondrial fragmentation is specific to pathogenic , it not observed nonpathogenic innocua species or several other intracellular pathogens. Strikingly, efficiency of affected in cells where either fission has...
Most archaea divide by binary fission using an FtsZ-based system similar to that of bacteria, but they lack many the divisome components described in model bacterial organisms. Notably, among multiple factors tether FtsZ membrane during cell constriction, only possess SepF-like homologs. Here, we combine structural, cellular, and evolutionary analyses demonstrate SepF is anchor human-associated archaeon Methanobrevibacter smithii. 3D super-resolution microscopy quantitative analysis...
Bacterial pathogens recruit clathrin upon interaction with host surface receptors during infection. Here, using three different infection models, we observed that host–pathogen interactions induce tyrosine phosphorylation of heavy chain. This modification was critical for recruitment actin at bacteria–host adhesion sites bacterial internalization or pedestal formation. At the interface, assembled to form coated pits conventional size. Because such structures cannot internalize large...
Segmented filamentous bacteria (SFB) are intestinal commensals that promote immune system development and pathogen protection through intimate attachment to the ileal epithelium. Attachment occurs via tip of unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), before outgrowth into filaments. To characterize this critical stage SFB life-cycle, we imaged using cryo-electron microscopy tomography. IOs were surrounded by a repetitive surface (S)-layer became replaced...
Stressed bacteria can enter a dormant viable but non-culturable (VBNC) state. VBNC pathogens pose an increased health risk as they are undetectable by growth-based techniques and wake up back into virulent Although widespread in bacteria, the mechanisms governing this phenotypic switch remain elusive. Here, we investigate state transition human pathogen Listeria monocytogenes. We show that starved mineral water become converting osmotically stable cell wall-deficient coccoid forms,...
The HIV-1 Env protein is exposed at the surface of virions and infected cells. fluctuates between different closed open structural states these conformations influence both viral infectivity sensitivity to antibody binding neutralization. We established a flow virometry assay visualize proteins human immunodeficiency virus type 1 (HIV-1) virions. performed on ultracentrifuged fluorescent particles that are stained with panel broadly neutralizing antibodies (bNAbs) nonneutralizing (nnAbs)...
We consider fluid wetting on a corrugated substrate using effective interfacial Hamiltonian theory and show that breaking the translational invariance along wall can induce an unbending phase transition in addition to unbinding. Both first-order second-order transitions occur at out of coexistence. Results for systems with short-ranged long-ranged forces establish critical point is characterized by hyperuniversal scaling behavior. that, bulk coexistence, adsorption universal multiple...
Recent technical developments allowed the accurate correlation of fluorescently labelled organelles in living cells to cryo-electron micrographs. We aimed at expanding this approach Plasmodium berghei sporozoites, motile forms a rodent malaria parasite, which can be imaged by tomography toto without need for sectioning. Sporozoites are crescent shaped eukaryotic that move on flat supports including EM grids circular, unidirectional manner. While sporozoites visualized with fluorescent light...
ABSTRACT Extracellular vesicles (EVs) are membrane-bound particles secreted by cells from all domains of life and implicated in a variety important processes, intercellular communication to pathogenesis. Here, we characterize EVs produced the dominant human gut methanogen, Methanobrevibacter smithii , which, unlike most archaea, contains peptidoglycan cell wall. Using quantitative proteomics, show that M. enriched various proteins responsible for chromatin structure, including histones, DNA...
Extract HTML view is not available for this content. However, as you have access to content, a full PDF via the ‘Save PDF’ action button. Extended abstract of paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4,
Extract HTML view is not available for this content. However, as you have access to content, a full PDF via the ‘Save PDF’ action button. Extended abstract of paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4,
We propose a theoretical investigation of the physical adsorption neutral comb-polymers with an adsorbing skeleton and non-adsorbing side-chains on flat surface. Such polymers are particularly interesting as "dynamic coating" matrices for bio-separations, especially DNA sequencing, capillary electrophoresis lab-on-chips. Separation performances increased by coating inner surface capillaries polymers. This method allows to screen charges, thus prevent electro-osmosis flow adhesion charged...
Abstract Cell-to-cell communication it is a fundamental mechanism by which unicellular and multicellular organisms maintain relevant functions as development or homeostasis. Tunneling nanotubes (TNTs) are type of contact-mediated cell-to-cell defined being membranous structures based on actin that allow the exchange different cellular material. TNTs have been shown to unique structural features compared with other protrusions contain cell adhesion molecule N-Cadherin. Here, we investigated...