A5079092710- Escherichia coli research studies
- Animal Nutrition and Physiology
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
- Livestock and Poultry Management
- Probiotics and Fermented Foods
- Coccidia and coccidiosis research
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Microbial infections and disease research
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Evolution and Genetic Dynamics
- Vibrio bacteria research studies
- Rabbits: Nutrition, Reproduction, Health
- Neuroendocrine Tumor Research Advances
Université de Tours
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Infectiologie Animale et Santé Publique
2014-2023
Biologie des Oiseaux et Aviculture
1999-2022
Institut National de la Recherche Agronomique
1996-2012
Centre Val de Loire
2004-2006
Département Génétique Animale
1994-1998
Laboratoire de Microbiologie et Génétique Moléculaires
1992
Extraintestinal pathogenic Escherichia coli (ExPEC) strains of human and avian origin show similarities that suggest the potentially have zoonotic properties. However, phylogenetic relationships between ExPEC are poorly documented, so this possibility is difficult to assess. We used PCR-based phylotyping multilocus sequence typing (MLST) determine 39 E. (APEC) serogroups O1, O2, O18, O78 51 strains. also compared virulence genotype pathogenicity for chickens APEC Twenty-eight 30 O18 were...
ABSTRACT In order to improve the identification of avian pathogenic Escherichia coli (APEC) strains, an extensive characterization 1,491 E. isolates was conducted, based on serotyping, virulence genotyping, and experimental pathogenicity for chickens. The originated from lesions colibacillosis ( n = 1,307) or intestines healthy animals 184) France, Spain, Belgium. A subset (460 isolates) this collection defined according their chicks. Six serogroups (O1, O2, O5, O8, O18, O78) accounted 56.5%...
ABSTRACT Extraintestinal pathogenic (ExPEC) Escherichia coli strains of serotype O18:K1:H7 are mainly responsible for neonatal meningitis and sepsis in humans belong to a limited number closely related clones. The same is also frequently isolated from the extraintestinal lesions colibacillosis poultry, but it not well known what extent human avian this particular related. Twenty-two ExPEC isolates origin 33 were compared on basis their virulence determinants, lethality chicks, pulsed-field...
bIL67 is a broad-host-range prolate-headed phage that active against Lactococcuscells. The complete genome sequence of 22195 bp was established. Thirty-seven open reading frames (ORFs) organized in two clusters were identified. Functions assigned to the putative products six ORFs on basis comparison deduced amino acid sequences known proteins, analysis structural features proteins and search for conserved motifs. These DNA polymerase, protein involved recombination, lysin, terminase subunit, holin.
The presence of ibeA, a gene encoding known virulence factor Escherichia coli strains responsible for neonatal meningitis in humans, was investigated the genome 213 avian pathogenic E. (APEC) and 55 non-pathogenic origin. Fifty-three were found to be ibeA +, all which belonged APEC group. is therefore positively linked pathogenicity (P<0·0001). Analysis serogroup revealed positive association with serogroups O18, O88 O2. On contrary, only 1/59 O78 are indicating negative this role strain BEN...
Avian pathogenic Escherichia coli (APEC) cause severe respiratory and systemic disease in chickens, commonly termed colibacillosis. Early immune responses after initial infection are highly important for the outcome of infection. In this study, early interactions between GFP-expressing APEC strains serotypes O1:K1:H7 O2:K1:H5 phagocytic cells lung CSF1R-reporter transgenic chickens were investigated. express fluorescent protein under control elements CSF1R promoter enhancer, such that...
The increase in antibiotic-resistant avian-pathogenic Escherichia coli (APEC), the causative agent of colibacillosis poultry, warrants urgent research and development alternative therapies. This study describes isolation characterization 19 genetically diverse, lytic coliphages, 8 which were tested combination for their efficacy controlling ovo APEC infections. Genome homology analysis revealed that phages belong to nine different genera, one them being a novel genus (Nouzillyvirus). One...
Locomotor disorders and infections by Escherichia coli represent major concerns to the poultry industry worldwide. Avian pathogenic E. (APEC) is associated with extraintestinal leading respiratory or systemic disease known as colibacillosis. The most common lesions seen in cases of colibacillosis are perihepatitis, airsacculitis, pericarditis, peritonitis/salpingitis arthritis. These diseases responsible for significant economic losses has been recently isolated from vertebral osteomyelitis...
Enterococcus cecorum is an important pathogen of poultry worldwide. It causes a number locomotor disorders and septicemia, particularly in fast-growing broilers.
Coccidiosis, a disease caused by intestinal apicomplexan parasites Eimeria, is threat to poultry production. Eimeria tenella one of the most pathogenic species, frequently causing high prevalence opportunistic infections.The objective this study investigate role microbiota in pathogenesis severe infection.We have previously shown that can promote parasite development. To effect on infection, we used an experimental condition (inoculum 10 000 oocysts E. INRAE) which load similar between...
Three genes coding for a type I R-M system related to the class C enzymes have been identified on chromosome of Lactococcus lactis strain IL1403. In addition, plasmids were found that encode only HsdS subunit directs specificity. The presence these in IL1403 conferred new phenotype host, indicating plasmid-encoded is able interact with chromosomally encoded HsdR and HsdM subunits. Such combinational variation systems may facilitate evolution their specificity thus reinforce bacterial...
Summary Heterogeneity of infection and extreme shedding patterns are common features animal infectious diseases. Individual hosts that super‐shedders key targets for control strategies. Nevertheless, the mechanisms associated with emergence remain largely unknown. During chicken salmonellosis, a high heterogeneity is observed when animal‐to‐animal cross‐contaminations reinfections reduced. We hypothesized unlike super‐shedders, low‐shedders would be able to block first Salmonella...
Coccidiosis is a widespread intestinal disease of poultry caused by parasite the genus Eimeria. Eimeria tenella, one most virulent species that specifically colonizes caeca, an organ which harbors rich and complex microbiota. Our objective was to study impact microbiota on infection development using original model germ-free broilers. We observed chickens presented significantly much lower load oocysts in caecal contents than conventional chickens. This decrease measurable tissue RT-qPCR at...
Stressed bacteria can enter a dormant viable but non-culturable (VBNC) state. VBNC pathogens pose an increased health risk as they are undetectable by growth-based techniques and wake up back into virulent Although widespread in bacteria, the mechanisms governing this phenotypic switch remain elusive. Here, we investigate state transition human pathogen Listeria monocytogenes. We show that starved mineral water become converting osmotically stable cell wall-deficient coccoid forms,...
: The number of people detained in French penitentiary establishments has never been as high 2024. This figure should not, however, mask the reality human rights country's tropism for confinement.
The chicken embryo has emerged as a popular in vivo model with increasing application biomedical research, due to its simplicity, affordability, and adaptability the study of various biological phenomena. This been used investigate microbial pathogenicity, is becoming useful tool bacterial dormancy. viable but non-culturable (VBNC) state dormant which bacteria become metabolically quiescent resistant cultivation preserve their viability harsh environments. Under favorable conditions, VBNC...
The complete nucleotide sequence and genetic organization of a new genomic island (AGI-3) isolated from the extraintestinal avian pathogenic Escherichia coli strain BEN2908 is reported. This 49,600-bp inserted at selC locus contains putative mobile elements such as phage-related integrase gene, transposase genes, direct repeats. AGI-3 shows mosaic structure five modules. Some these modules are present in other E. strains bacterial species. gene cluster aec-35 to aec-37 module 1 encodes...
We identified a carbohydrate metabolic operon (frz) that is highly associated with extraintestinal pathogenic Escherichia coli (ExPEC) strains. The frz codes for three subunits of phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) transporter the fructose subfamily, transcriptional activator PTSs MgA family, two type II ketose-1,6-bisphosphate aldolases, sugar-specific kinase (repressor, open reading frame, family [ROK]), and protein cupin superfamily. proved promotes bacterial...
Avian pathogenic Escherichia coli (APEC) causes colibacillosis, the main bacterial disease in poultry leading to significant economic losses worldwide. Antibiotic treatments favor emergence of multidrug-resistant bacteria, and preventive measures are insufficient control disease. There is increasing interest using potential bacteriophages, not only for phage therapy but also prevention biocontrol. This study aimed evaluate efficacy a cocktail administered ovo prevent avian colibacillosis...
Prebiotics such as fructooligosaccharides (FOS) are increasingly being used in some countries for improving human and animal health an alternative to antibiotic growth promoters animals, with various degrees of success. It has been observed that FOS stimulate the proliferation probiotic bacteria and, at same time, decrease population associated disease. This observation assumes pathogenic do not metabolize therefore, lose their competitive advantage over beneficial bacteria. Here we present...
Background Although antimicrobial resistance is increasingly common in equine medicine, molecular and epidemiological data remains scarce. Objectives We estimated the prevalence of, risk factors for, shedding of multidrug resistant (MDR), extended spectrum β‐lactamase (ESBL)‐producing, AmpC β‐lactamase‐producing, or some combination these Escherichia coli horses France. characterized ESBL/AmpC isolates for susceptibility presence virulence ESBL/AmpC‐associated genes. Animals Fecal samples...