A5037901255- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Genetic and phenotypic traits in livestock
- Immune Response and Inflammation
- Amoebic Infections and Treatments
- Animal Genetics and Reproduction
- Animal Nutrition and Physiology
- Toxoplasma gondii Research Studies
- Pediatric health and respiratory diseases
- Infant Nutrition and Health
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Neuropeptides and Animal Physiology
- Veterinary medicine and infectious diseases
- Milk Quality and Mastitis in Dairy Cows
- Neonatal Respiratory Health Research
- Veterinary Equine Medical Research
- T-cell and Retrovirus Studies
- Genetic Mapping and Diversity in Plants and Animals
- Antimicrobial Peptides and Activities
- Helminth infection and control
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Meat and Animal Product Quality
- Cytomegalovirus and herpesvirus research
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
1997-2024
Infectiologie Animale et Santé Publique
2015-2024
Université de Tours
2015-2024
Centre Val de Loire
2000-2024
Ipso Facto (France)
2023
Astrophysique, Instrumentation et Modélisation
2018
Arvalis - Institut du Végétal
2005-2018
Physiologie de la Reproduction et des Comportements
2018
Institut National de la Recherche Agronomique
1999-2015
Département Santé Animale
2014
Abstract Giardia lamblia infection of the human small intestine is a common protozoan cause diarrheal disease worldwide. Although luminal and generally self-limiting, secretory Abs are thought to be important in host defense, other defense mechanisms probably affect duration severity symptoms. Because intestinal epithelial cells produce NO, its stable end products, nitrite nitrate, detectable mainly on apical side, we tested hypothesis that NO production may constitute against G. lamblia....
ABSTRACT The recent cloning of chicken genes coding for interleukins, chemokines, and other proteins involved in immune regulation inflammation allowed us to analyze their expression during infection with Eimeria . levels different jejunal cecal RNA extracts isolated from uninfected chickens infected maxima or E. tenella were measured using a precise quantitative reverse transcription-PCR technique. Seven days after infection, the proinflammatory cytokine interleukin-1β (IL-1β) mRNA was...
Comparative genomics studies are central in identifying the coding and non-coding elements associated with complex traits, functional annotation of genomes is a critical step to decipher genotype-to-phenotype relationships livestock animals. As part Functional Annotation Animal Genomes (FAANG) action, FR-AgENCODE project aimed create reference maps domesticated animals by profiling landscape transcription (RNA-seq), chromatin accessibility (ATAC-seq) conformation (Hi-C) species representing...
Cryptosporidium parvum induces moderate levels of apoptosis cultured human intestinal epithelial cells, which are maximal at 24 h after infection. Apoptosis is further increased in C. parvum-infected cells by inhibition NF-kappaB. infection also attenuates induced strongly proapoptotic agents. The data suggest has developed strategies to limit order facilitate its growth and maturation the early period cell
Cryptosporidium parvum infects intestinal epithelial cells and does not invade deeper layers of the mucosa. Nonetheless, an inflammatory cell infiltrate that consists neutrophils mononuclear is often present in lamina propria, which underlies epithelium. This study investigated host response to C. by assessing vitro vivo expression production proinflammatory cytokines after infection. The human colon lines HCT-8 Caco-2 xenografts SCID mice were infected with parvum. secretion C-X-C...
Cryptosporidium parvum is a zoonotic protozoan parasite found worldwide, that develops only in the gastrointestinal epithelium and causes profuse diarrhea. Using mouse model of C. infection, we demonstrated by conditional depletion CD11c+ cells these are essential for control infection both neonates adults. Neonates highly susceptible to but self-limited, whereas adults resistant unless immunocompromised. We investigated contribution DC age-dependent susceptibility infection. presented...
Both neonatal and C57BL/6 gamma interferon (IFN-gamma) knockout (C57BL/6-GKO) mice are susceptible to Cryptosporidium parvum, but the course of infection is different. Neonatal able clear parasite within 3 weeks, whereas C57BL/6-GKO mice, depending on age, die rapidly or remain chronically infected. The mechanism by which IFN-gamma leads a protective immunity yet poorly understood. In order investigate effect other cytokines expressed in intestinal mucosa during C. parvum infection, we...
Abstract Protease-activated receptor-2 (PAR2), a receptor highly expressed in the respiratory tract, can influence inflammation at mucosal surfaces. Although effects of PAR2 innate immune response to bacterial infection have been documented, knowledge its role context viral is lacking. We thus investigated influenza pathogenesis vitro and vivo. In vitro, stimulation on epithelial cells inhibited virus type A (IAV) replication through production IFN-γ. vivo, using specific agonists protected...
Targeting parasitic endonuclease CPSF3 with an oxaborole offers a potent and selective candidate treatment against cryptosporidiosis.
Coccidiosis, a disease caused by intestinal apicomplexan parasites Eimeria, is threat to poultry production. Eimeria tenella one of the most pathogenic species, frequently causing high prevalence opportunistic infections.The objective this study investigate role microbiota in pathogenesis severe infection.We have previously shown that can promote parasite development. To effect on infection, we used an experimental condition (inoculum 10 000 oocysts E. INRAE) which load similar between...
ABSTRACT Cryptosporidium parvum is a protozoan parasite that infects intestinal epithelial cells and induces inflammation of the intestine. To better understand inflammatory process occurring during cryptosporidiosis, we investigated in this study kinetics chemokine expression mucosa mice by quantitative reverse transcription-PCR. Our results demonstrate among mRNAs studied, gamma interferon (IFN-γ)-inducible protein 10 (IP-10), monokine induced IFN-γ (MIG), i-TAC, lymphotactin, macrophage...
ABSTRACT Cryptosporidium parvum is an important cause of diarrhea in humans and several animal species. Prostaglandins play a central role regulating intestinal fluid secretion models cryptosporidiosis, but their cellular sources mechanisms induction are unclear. Here, we show that C. infection directly activates prostaglandin H synthase 2 expression E F 2α production human epithelial cells.
The neonatal intestinal immune system is still undergoing development at birth, leading to a higher susceptibility mucosal infections. In this study, we investigated the effect of poly(I:C) on controlling enteric infection by protozoan Cryptosporidium parvum in mice. After administration, rapid reduction parasite burden was observed and proved be dependent CD11c+ cells TLR3/TRIF signaling. Protection against C. required additional signals provided gut flora through TLR5 MyD88 This...
CCL20 is a chemokine with antimicrobial activity. We investigated its expression and role during neonatal cryptosporidiosis, worldwide protozoan enteric disease leading to severe diarrhea. Surprisingly, infection by Cryptosporidium parvum, production the intestine of mice reduced mechanism independent both flora interferon γ, key cytokine for resolution this infection. However, oral administration recombinant significantly parasite load that was immune cell recruitment occurred instead...
Eimeria tenella infection leads to acute intestinal disorders responsible for important economic losses in poultry farming worldwide. The life-cycle of E. is monoxenous with the chicken as exclusive host; occurs caecal epithelial cells. However, vitro, complete parasite has only been propagated successfully primary kidney cells, which comprise undefined mixed cell populations; no line model able consistently support development sexual stages parasite. We therefore sought develop a new study...
Coccidiosis is a widespread intestinal disease of poultry caused by parasite the genus Eimeria. Eimeria tenella, one most virulent species that specifically colonizes caeca, an organ which harbors rich and complex microbiota. Our objective was to study impact microbiota on infection development using original model germ-free broilers. We observed chickens presented significantly much lower load oocysts in caecal contents than conventional chickens. This decrease measurable tissue RT-qPCR at...
The Apicomplexa comprise a large phylum of single-celled, obligate intracellular protozoa that include Toxoplasma gondii , Plasmodium and Cryptosporidium spp., which infect humans animals cause severe parasitic diseases. Available therapeutics against these diseases are limited by suboptimal efficacy frequent side effects, as well the emergence spread resistance. We use drug repurposing strategy identify altiratinib, compound originally developed to treat glioblastoma, promising candidate...
Neonates are particularly vulnerable to infections, in part because of the incomplete development their immune system. Recent advances immunostimulatory treatments based on conserved microbial components led us assess potential oligodeoxynucleotides (ODNs) for decreasing sensitivity neonates Cryptosporidium parvum infection.Neonate mice were treated orally or intraperitoneally (ip) with CpG ODNs non-CpG 24 h before C. infection, and parasite load cytokine up-regulation evaluated.CpG ODN 1668...
Intestinal epithelial cells form a single layer separating the intestinal lumen containing nutriments and microbiota from underlying sterile tissue therefore play key role in maintaining homeostasis. We investigated factors contributing to alteration of barrier function during Cryptosporidium parvum infection. Infected polarized cell monolayers exhibit drop transepithelial resistance associated with delocalization E-cadherin β-catenin their intercellular area contact, adherens junction...