A5028527760- Blood Coagulation and Thrombosis Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Gastrointestinal motility and disorders
- Neuropeptides and Animal Physiology
- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- Metabolism and Genetic Disorders
- Helicobacter pylori-related gastroenterology studies
- Complement system in diseases
- Pulmonary Hypertension Research and Treatments
- Congenital Heart Disease Studies
- Platelet Disorders and Treatments
- Gut microbiota and health
- Diet and metabolism studies
- Pancreatitis Pathology and Treatment
- Protease and Inhibitor Mechanisms
- Dermatology and Skin Diseases
- Inflammatory Bowel Disease
- Digestive system and related health
- Apelin-related biomedical research
- Cell Adhesion Molecules Research
- Porphyrin Metabolism and Disorders
- Urinary Bladder and Prostate Research
- S100 Proteins and Annexins
- Urticaria and Related Conditions
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
University of Calgary
2013-2025
Université Toulouse III - Paul Sabatier
2016-2025
Université de Toulouse
2016-2025
Inserm
2016-2025
Digestive Health Research Institute
2016-2025
École Nationale Vétérinaire de Toulouse
2016-2025
Centre National de la Recherche Scientifique
2011-2023
Unité de Technologies Chimiques et Biologiques pour la Santé
2022
Centre Hospitalier Universitaire de Toulouse
2010-2021
Mediators involved in the generation of symptoms patients with irritable bowel syndrome (IBS) are poorly understood. Here we show that colonic biopsy samples from IBS release increased levels proteolytic activity (arginine cleavage) compared to asymptomatic controls. This was dependent on activation NF-kappaB. In addition, measured vivo, washes control patients. Trypsin and tryptase expression were biopsies subjects. Biopsies (but not controls) released mediators sensitized murine sensory...
Inflammatory proteases (mast cell tryptase and trypsins) cleave protease-activated receptor 2 (PAR ) on spinal afferent neurons cause persistent inflammation hyperalgesia by unknown mechanisms. We determined whether transient potential vanilloid 1 (TRPV1), a cation channel activated capsaicin, protons, noxious heat, mediates PAR -induced hyperalgesia. was coexpressed with TRPV1 in small- to medium-diameter of the dorsal root ganglia (DRG), as immunofluorescence. agonists increased...
Exacerbated sensitivity to mechanical stimuli that are normally innocuous or mildly painful (mechanical allodynia and hyperalgesia) occurs during inflammation underlies diseases. Proteases generated disease cleave protease‐activated receptor 2 (PAR ) on afferent nerves cause hyperalgesia in the skin intestine by unknown mechanisms. We hypothesized PAR ‐mediated requires sensitization of ion channel transient potential vanilloid 4 (TRPV4). Immunoreactive TRPV4 was coexpressed rat dorsal root...
Lactic acid–producing bacteria engineered to produce the antiprotease Elafin restore colon homeostasis in mice with colitis and protect human tissue from inflammation.
Proteases that are released during inflammation and injury cleave protease-activated receptor 2 (PAR2) on primary afferent neurons to cause neurogenic hyperalgesia. PAR2-induced thermal hyperalgesia depends sensitization of transient potential vanilloid 1 (TRPV1), which is gated by capsaicin, protons noxious heat. However, the signalling mechanisms PAR2 sensitizes TRPV1 not fully characterized. Using immunofluorescence confocal microscopy, we observed was colocalized with protein kinase (PK)...
Proteinase-activated receptor 2 (PAR2) has been suggested to play a role in inflammatory reactions. Because leukocyte-endothelial cell interactions are critical events during reactions, and because PAR2 is expressed both on endothelium leukocytes, we have examined the effects of PAR2-activating peptides (PAR2-APs) leukocyte rolling adhesion mesenteric venules recruitment into peritoneal cavity. Using intravital microscopy, rolling, flux, rat postcapillary were quantified. Topical addition...
In the present study, we have observed development of an inflammatory reaction in rat hindpaw, following injection specific agonists PAR 2 (two activating peptides). This inflammation was characterized by oedema and granulocyte infiltration. Two selective peptides, SLGRL‐NH trans ‐cinnamoyl‐LIGRLO‐NH induced significant hindpaw from 1–6 h subplantar injection. Six hours after ‐activating peptide injection, paw tissues showed a complete disruption tissue architecture along with cell...
The proteinase-activated receptor 2 (PAR-2) is a member of family G protein-coupled receptors for proteases. Proteases cleave PARs within the extracellular N-terminal domains to expose tethered ligands that bind and activate cleaved receptors. PAR-2 highly expressed in colon epithelial neuronal elements. In this study we show activation prevents development induces healing T helper cell type 1-mediated experimental colitis induced by intrarectal administration 2,4,6-trinitrobenzene sulfonic...
Citrobacter rodentium is a bacterial pathogen that causes murine infectious colitis equivalent to enterohemorrhagic Escherichia coli infection in humans. Colonic luminal fluid from C. -infected mice, but not sham-infected contains active serine proteinases can activate proteinase-activated receptor-2 (PAR 2 ). We have identified granzyme A and trypsins be present fluid, as determined by mass spectrometry Western blot analysis. Inflammatory indices (colonic mucosa macroscopic damage score,...
Transient Receptor Potential Melastatin–8 (TRPM8), a recently identified member of the transient receptor potential (TRP) family ion channels, is activated by mild cooling and chemical compounds such as supercooling agent, icilin. Since cooling, possibly involving TRPM8 stimulation, diminishes injury-induced peripheral inflammation, we hypothesized that activation may also attenuate systemic inflammation. We thus studied involvement in regulating colonic inflammation using two mouse models...
<h3>Background</h3> Although evidence points to a role for histamine and serotonin in visceral hypersensitivity, activation of calcium channels such as transient receptor potential vanilloid 4 (TRPV4) also causes hypersensitivity. We hypothesised that TRPV4 is important the generation mediating histamine- serotonin-induced <h3>Methods</h3> In response histamine, and/or agonist (4αPDD), signals localisation studies were performed on dorsal root ganglia (DRG) neurons projecting from colon. To...