A5042025873- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Esophageal Cancer Research and Treatment
- Esophageal and GI Pathology
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Gastric Cancer Management and Outcomes
- Helminth infection and control
- Acute Lymphoblastic Leukemia research
- Multiple Sclerosis Research Studies
- Lung Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
- Energetic Materials and Combustion
- Animal Nutrition and Physiology
- Systemic Sclerosis and Related Diseases
- Bipolar Disorder and Treatment
- Lysosomal Storage Disorders Research
- Meat and Animal Product Quality
- Electrochemical Analysis and Applications
- Glycogen Storage Diseases and Myoclonus
- Steroid Chemistry and Biochemistry
- Solidification and crystal growth phenomena
- Insect and Pesticide Research
- Neonatal Respiratory Health Research
Novartis (Australia)
2018-2024
Teva Pharmaceuticals (Australia)
2018-2023
Queen Elizabeth Hospital Birmingham
2021-2022
University Hospital Southampton NHS Foundation Trust
2021
Australian Institute of Tropical Health and Medicine
2013-2020
James Cook University
2012-2020
Manchester University NHS Foundation Trust
2020
Royal Manchester Children's Hospital
2009-2020
University of Zurich
2015-2019
University of Otago
2019
Abstract Almost any warm-blooded creature can be an intermediate host for Toxoplasma gondii . However, sexual reproduction of T occurs only in felids, wherein fertilisation haploid macrogametes by microgametes, results diploid zygotes, around which a protective wall develops, forming unsporulated oocysts. Unsporulated oocysts are shed the faeces cats and meiosis gives rise to sporozoites within These, now infectious, sporulated contaminate environment as source infection people their...
The oocyst wall of coccidian parasites is a robust structure that resistant to variety environmental and chemical insults. This resilience allows oocysts survive for long periods, facilitating transmission from host host. bilayered formed by the sequential release contents two specialized organelles - forming body 1 2 found in macrogametocyte stage Coccidia. over 90% protein but few these proteins have been studied. One group cysteine-rich may be presumed crosslink via disulphide bridges,...
The apicomplexan parasite Toxoplasma gondii is cosmopolitan in nature, largely as a result of its highly flexible life cycle. Felids are only definitive hosts and wide range mammals birds serve intermediate hosts. latent bradyzoite stage orally infectious all warm-blooded vertebrates establishes chronic, transmissible infections. When bradyzoites ingested by felids, they transform into merozoites enterocytes expand asexually part their coccidian In other hosts, however, differentiate...
The protozoan Eimeria tenella is a common parasite of chickens, causing avian coccidiosis, disease on-going concern to agricultural industries. high prevalence E. can be attributed the resilient oocyst stage, which transmitted between hosts in environment. As related Coccidia, development eimerian appears dependent on completion parasite’s sexual cycle. RNA Seq transcriptome profiling offers insights into mechanisms governing biology stages (gametocytes) and potential identify targets for...
Toxoplasma gondii undergoes many phenotypic changes during its life cycle. The recent identification of AP2 transcription factors in T. has provided a platform for studying the mechanisms controlling gene expression. In present study, we report that recombinant protein encompassing TgAP2XI-4 domain was able to specifically bind DNA motif using gel retardation assays. is localized parasite nucleus throughout tachyzoite cycle vitro, with peak expression occurring after cytokinesis. We found...
Eimeria tenella infection leads to acute intestinal disorders responsible for important economic losses in poultry farming worldwide. The life-cycle of E. is monoxenous with the chicken as exclusive host; occurs caecal epithelial cells. However, vitro, complete parasite has only been propagated successfully primary kidney cells, which comprise undefined mixed cell populations; no line model able consistently support development sexual stages parasite. We therefore sought develop a new study...
Background Apicomplexan parasites are responsible for some of the most deadly parasitic diseases afflicting humans, including malaria and toxoplasmosis. These obligate intracellular exhibit a complex life cycle coordinated cell cycle-dependant expression program. Their division is multistep process. How this mechanism organised remains poorly understood. Methods Findings In study, we provide evidence link between heterochromatin, compartmentalisation nucleus in Toxoplasma gondii. We...
Abstract Background Proteases regulate pathogenesis in apicomplexan parasites but investigations of proteases have been largely confined to the asexual stages Plasmodium falciparum and Toxoplasma gondii . Thus, little is known about other Apicomplexa, particularly sexual stages. We screened Eimeria tenella genome database for proteases, classified these into families determined their stage specific expression. Results Over forty protease genes were identified E. genome. These distributed...
Background Oral infection of C57BL/6J mice with the protozoan parasite Toxoplasma gondii leads to a lethal inflammatory ileitis. Principal Findings Mice lacking purinergic receptor P2X7R are acutely susceptible toxoplasmic ileitis, losing significantly more weight than and exhibiting much greater intestinal pathology in response only 10 cysts T. gondii. This susceptibility is not dependent on ability P2X7R-deficient control parasite, which they accomplish just as efficiently mice. Rather,...
Contemporary information relating to the prevalence of Toxoplasma gondii in humans is lacking for UK population, with even less available about human parasite Scotland. To address this, two different study groups were used determine and genotypes Scottish population. The first group included serum samples from blood donors (n = 3273) over a four-year period (2006–2009) second comprised DNA extracted brains 151) five-year (2008–2012). A T. IgG ELISA was performed seroprevalence sera...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA General Synthesis of 4,5-Unsaturated 2-Oxasteroids. A 2-OxacortisolRalph. Hirschmann, N. G. Steinberg, and Robert. WalkerCite this: J. Am. Chem. Soc. 1962, 84, 7, 1270–1278Publication Date (Print):April 1, 1962Publication History Published online1 May 2002Published inissue 1 April 1962https://pubs.acs.org/doi/10.1021/ja00866a043https://doi.org/10.1021/ja00866a043research-articleACS PublicationsRequest reuse permissionsArticle...
Three cases of tetanus occurring in a community southern West Virginia five years prompted survey the immunization status population this region. An history was obtained from 540 consecutive patients seen at three different health care facilities. Of these, 386 (71.5%) had received prior protection, 65 (12%) never been immunized, and 89 (16.5%) were uncertain or incomplete immunization. nonimmunized patients, 54 (83%) older than 50 only 11 (17%) younger. Compared to total group sampled,...
ABSTRACT Sexual-stage glycoproteins of Eimeria are important components the oocyst wall, a structure that ensures efficient transmission these and related parasites. In this study, primary enzyme in glycosylation pathway tenella , glucosamine:fructose-6-phosphate aminotransferase (EtGFAT), has been characterized as macrogamete-specific protein. Although transcription Et GFAT was observed early macrogamete development, protein expression restricted to mature macrogametes, prior their...
Abstract Toxoplasmic encephalitis is an AIDS-defining condition. The decline of IFN-γ-producing CD4 + T cells in AIDS a major contributing factor reactivation quiescent Toxoplasma gondii to actively replicating stage infection. Hence, it important characterize CD4-independent mechanisms that constrain acute T. We investigated the vivo regulation IFN-γ production by CD8 cells, DN and NK response Our data show processing these non-CD4 dependent on both IL-12 IL-18 secretion bioactive requires...
Background: Evidence from network meta-analyses (NMAs) and real-world propensity score (PS) analyses suggest monoclonal antibodies (mAbs) offer a therapeutic advantage over currently available oral therapies and, therefore, warrant consideration as distinct group of high-efficacy disease-modifying (DMTs) for patients with relapsing multiple sclerosis (RMS). This is counter to the current perception these by some stakeholders, including payers. Objectives: A multifaceted indirect treatment...
Eimeria is an important genus of apicomplexan parasites. A defining feature these parasites the oocyst, which transmitted into environment via faeces definitive hosts. The oocyst wall contains cross-linked, tyrosine-rich proteins and protects eight infectious sporozoites, housed in pairs within a second walled structure, sporocyst. biochemical basis for sporocyst formation not known. Here, we report discovery novel protein, EtSWP1, tenella. Like wall, EtSWP1 intrinsically disordered protein...
Background Treatment of MS often begins with low-efficacy injectable disease-modifying therapy (iDMT). Objectives To compare the effect fingolimod 0.5 mg/day on clinical, MRI, patient-reported, and safety outcomes, in treatment-naïve previously treated (≥1 iDMT) patients early MS. Methods EARLIMS was a multicentre, open-label, non-randomized, parallel-group phase 3 b/4 study Australia Spain. Patients relapsing–remitting MS, Expanded Disability Status Scale (EDSS) score <4.0, ≥1–5 years...