A5002603079- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Parasitic Infections and Diagnostics
- Invertebrate Immune Response Mechanisms
- Bacteriophages and microbial interactions
- Malaria Research and Control
- RNA Interference and Gene Delivery
- interferon and immune responses
- HIV Research and Treatment
- Autophagy in Disease and Therapy
- Polyamine Metabolism and Applications
- RNA modifications and cancer
- Microbial infections and disease research
- Genomics and Chromatin Dynamics
- Genetics, Bioinformatics, and Biomedical Research
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Trypanosoma species research and implications
- Calcium signaling and nucleotide metabolism
- Mitochondrial Function and Pathology
- Insect Resistance and Genetics
Center for Infection and Immunity of Lille
2013-2025
Institut Pasteur de Lille
2012-2025
Inserm
2014-2025
Centre Hospitalier Universitaire de Lille
2016-2025
Université de Lille
2016-2025
Centre National de la Recherche Scientifique
2013-2025
Institut Pasteur
2012-2014
Université Lille Nord de France
2012-2013
Albert Einstein College of Medicine
2007-2011
Yeshiva University
2008-2011
Mechanisms of gene regulation are poorly understood in Apicomplexa, a phylum that encompasses deadly human pathogens like Plasmodium and Toxoplasma. Initial studies suggest epigenetic phenomena, including histone modifications chromatin remodeling, have profound effect upon expression virulence traits. Using the model organism Toxoplasma gondii, we characterized organization transcription patterns contiguous 1% T. gondii genome using custom oligonucleotide microarrays. We show methylation...
Members of the eukaryotic phylum Apicomplexa are cause important human diseases including malaria, toxoplasmosis, and cryptosporidiosis. These obligate intracellular parasites produce new invasive stages through a complex budding process. The cycle is remarkably flexible can varied numbers progeny to adapt different host-cell niches. How this process coordinated remains poorly understood. Using Toxoplasma gondii as genetic model, we show that key element coordination centrocone, unique...
Toxoplasma gondii undergoes many phenotypic changes during its life cycle. The recent identification of AP2 transcription factors in T. has provided a platform for studying the mechanisms controlling gene expression. In present study, we report that recombinant protein encompassing TgAP2XI-4 domain was able to specifically bind DNA motif using gel retardation assays. is localized parasite nucleus throughout tachyzoite cycle vitro, with peak expression occurring after cytokinesis. We found...
Toxoplasma gondii virulence depends on the expression of factors packed into specific organelles such as rhoptry and microneme. Although factor is tightly regulated, molecular mechanisms controlling their regulation remain poorly understood. ApiAP2 are a family conserved transcription (TFs) that play an important role in regulating gene apicomplexan parasites. TgAP2XI-5 able to bind transcriptionally active promoters genes expressed during S/M phase cell cycle, (rhoptries micronemes genes)....
Apicomplexan parasites have evolved efficient and distinctive strategies for intracellular replication where the timing of emergence daughter cells (budding) is a decisive element. However, molecular mechanisms that provide proper parasite budding remain unknown. Using Toxoplasma gondii as model Apicomplexan, we identified master regulator controls process. We show an ApiAP2 transcription factor, TgAP2IX-5, cell cycle events downstream centrosome duplication. TgAP2IX-5 binds to promoter...
Programmed-cell death is an antimicrobial defense mechanism that promotes clearance of intracellular pathogens. Toxoplasma counteracts host immune defenses by secreting effector proteins into cells; however, how the parasite evades lytic cell and effectors involved remain poorly characterized. We identified ROP55, a rhoptry protein survival preventing in absence IFN-γ stimulation. RNA-Seq analysis revealed ROP55 acts as repressor pro-inflammatory responses. In THP-1 monocytes ΔROP55...
Epigenetic factors play a role in the expression of virulence traits Apicomplexa. Apicomplexan genomes encode putative DNA cytosine methylation enzymes. To assess presence Toxoplasma gondii and Cryptosporidium parvum DNA, we used mass spectrometry analysis confirmed that these organisms lack detectable methylcytosine their DNA.
Toxoplasma gondii parasites cause toxoplasmosis, arguably the most widespread and prevalent parasitosis of humans animals. During clinically relevant stage its life cycle, divide by endodyogeny.
Toxoplasmosis is a neglected parasitic disease necessitating public health control. Host cell invasion by Toxoplasma occurs at different stages of the parasite's life cycle and crucial for survival establishment infection. In tachyzoites, which are responsible acute toxoplasmosis, involves formation molecular bridge between parasite host membranes, referred to as moving junction (MJ). The MJ shaped assembly AMA1 RON2, part complex involving additional RONs. While this essential process well...
Coordination between nucleus and mitochondria is essential for cell survival, thus numerous communication routes have been established these two organelles over eukaryotic evolution. One route organelle via membrane contact sites, functional appositions formed by molecular tethers. We describe a novel nuclear-mitochondrial site in the protozoan Toxoplasma gondii. identified specific contacts occurring at nuclear pore demonstrated an interaction components of mitochondrial protein translocon,...
Representations of the genome can be generated by selection a subpopulation restriction fragments using ligation-mediated PCR. Such representations form basis for number high-throughput assays, including HELP assay to study cytosine methylation. We find that data analysis is complicated not only PCR amplification heterogeneity but also complex and variable distribution To address this, we created an analytical pipeline novel normalization approach improves concordance between...
Background Apicomplexan parasites are responsible for some of the most deadly parasitic diseases afflicting humans, including malaria and toxoplasmosis. These obligate intracellular exhibit a complex life cycle coordinated cell cycle-dependant expression program. Their division is multistep process. How this mechanism organised remains poorly understood. Methods Findings In study, we provide evidence link between heterochromatin, compartmentalisation nucleus in Toxoplasma gondii. We...
Toxoplasma gondii is a eukaryotic parasite that forms latent cysts in the brain of immunocompetent individuals. The infection immune-privileged central nervous system linked to most complications. With no drug currently available eliminate infected hosts, consequences neurons' long-term are unknown. It has long been known T. specifically differentiates into form (bradyzoite) neurons, but how neuron responds remains be elucidated. We have established new vitro model resulting production...
ABSTRACT In eukaryotes, the high-mobility-group (HMG) nuclear factors are highly conserved throughout evolution and divided into three families, including HGMB, characterized by an HMG box domain. Some HMGB DNA structure specific preferentially interact with distorted sequences, trigger bending, hence facilitate binding of nucleoprotein complexes that in turn activate or repress transcription. Plasmodium falciparum , two were predicted: PfHMGB1 PfHMGB2. They small proteins, under 100 amino...
The sexual cycle of Plasmodium is required for transmission malaria from mosquitoes to mammals, but how parasites induce the expression genes stages not known. We disrupted yoelii gene encoding high mobility group nuclear factor hmgb2, which encodes a DNA-binding protein potentially implicated in transcriptional regulation expression. investigated its function vivo vertebrate and invertebrate hosts. Deltapyhmgb2 develop into gametocytes have drastic impairment oocyst formation. A global...
Expansion microscopy (ExM) is an innovative approach to achieve super-resolution images without using microscopes, based on the physical expansion of sample. The advent ExM has unlocked detail for a broader scientific circle, lowering cost and entry skill requirements field. One its branches, ultrastructure (U-ExM), become popular among research groups studying apicomplexan parasites, including acute stage
Toxoplasma gondii is a unicellular eukaryotic pathogen that belongs to the Apicomplexa phylum, which encompasses some of deadliest pathogens medical and veterinary importance. The centrosome key organisation coordination cell cycle division apicomplexan parasites. T. possesses particular bipartite structure (outer inner cores). One main roles ensure proper karyokinesis. However, how these 2 events are coordinated still unknown in gondii, for components poorly described. To gain more insights...