A5059039151- Mitochondrial Function and Pathology
- Toxoplasma gondii Research Studies
- Metabolism and Genetic Disorders
- Metalloenzymes and iron-sulfur proteins
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Autophagy in Disease and Therapy
- Metal-Catalyzed Oxygenation Mechanisms
- Plant Parasitism and Resistance
- Plant Stress Responses and Tolerance
- interferon and immune responses
- Microbial Metabolic Engineering and Bioproduction
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- ATP Synthase and ATPases Research
- Biochemical Acid Research Studies
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Herpesvirus Infections and Treatments
- Legume Nitrogen Fixing Symbiosis
- Trace Elements in Health
- Malaria Research and Control
- Electrocatalysts for Energy Conversion
University of Glasgow
2019-2024
Wellcome Centre for Molecular Parasitology
2019-2024
John Innes Centre
2015-2021
University of East Anglia
2017-2021
Norwich Research Park
2018
The mitochondrial electron transport chain (mETC) and F 1 o -ATP synthase are of central importance for energy metabolism in eukaryotic cells. Apicomplexa, important pathogens humans causing diseases such as toxoplasmosis malaria, depend on their mETC every known stage complicated life cycles. Here, using a complexome profiling proteomic approach, we have characterised the Toxoplasma complexes synthase. We identified assigned 60 proteins to II, IV , which 16 not been previously. Notably, our...
During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double nramp3 nramp4 mutant unable to mobilize Fe stores does not develop absence of external Fe. We used RNA sequencing compare gene expression wild type during germination early seedling development. Even though sufficient was supplied, Fe-responsive transcription factors bHLH38, 39, 100, 101 their downstream targets FRO2 IRT1 mediating uptake were strongly...
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Summary Apicomplexan parasites cause diseases such as malaria and toxoplasmosis. The apicomplexan mitochondrion shows striking differences from common model organisms, including fundamental processes mitochondrial translation. Despite evidence that translation is essential for parasite survival, it largely understudied. Progress has been restricted by the absence of functional assays to detect translation, a lack knowledge proteins involved in process inability identify mitoribosomes. We...
Coordination between nucleus and mitochondria is essential for cell survival, thus numerous communication routes have been established these two organelles over eukaryotic evolution. One route organelle via membrane contact sites, functional appositions formed by molecular tethers. We describe a novel nuclear-mitochondrial site in the protozoan Toxoplasma gondii. identified specific contacts occurring at nuclear pore demonstrated an interaction components of mitochondrial protein translocon,...
The mitochondrial electron transport chain (mETC) is a series of membrane embedded enzymatic complexes critical for energy conversion and metabolism. In commonly studied eukaryotes, including humans animals, complex II, also known as succinate dehydrogenase (SDH), an essential four-subunit enzyme that acts entry point to the mETC, by harvesting electrons from TCA cycle. Apicomplexa are pathogenic parasites with significant impact on human animal health. phylum includes Toxoplasma gondii...
Abstract Iron–sulfur (Fe–S) clusters are ubiquitous cofactors in all life and used a wide array of diverse biological processes, including electron transfer chains several metabolic pathways. Biosynthesis machineries for Fe–S exist plastids, the cytosol, mitochondria. A single monothiol glutaredoxin (GRX) is involved cluster assembly mitochondria yeast mammals. In plants, role mitochondrial homolog GRXS15 has only partially been characterized. Arabidopsis (Arabidopsis thaliana) grxs15 null...
Mitochondria play a key role in the biosynthesis of two metal cofactors, iron-sulfur (FeS) clusters and molybdenum cofactor (Moco). The pathways intersect at several points, but scarcity mutants has hindered studies to better understand these links. We screened collection sirtinol-resistant Arabidopsis thaliana for lines with decreased activities cytosolic FeS enzymes Moco enzymes. identified new mutant allele ATM3 (ABC transporter mitochondria 3), encoding ATP-binding cassette 3 (systematic...
Abstract Iron-sulfur (Fe-S) clusters are ubiquitous inorganic cofactors required for numerous essential cellular pathways. Since they cannot be scavenged from the environment, Fe-S synthesised de novo in compartments such as apicoplast, mitochondrion and cytosol. The cytosolic cluster biosynthesis pathway relies on transport of an intermediate mitochondrial pathway. An ATP binding cassette (ABC) transporter called ABCB7 is responsible this role commonly studied organisms, but its medically...
Iron-sulfur (Fe-S) clusters are ubiquitous inorganic cofactors required for numerous essential cellular pathways. Since they cannot be scavenged from the environment, Fe-S synthesized
Complex I deficiency is a common cause of mitochondrial disease, resulting from mutations in genes encoding structural subunits, assembly factors or defects gene expression. Advances genetic diagnostics and sequencing have led to identification several variants NUBPL (nucleotide binding protein-like), an factor complex I, which are potentially pathogenic. To help assign pathogenicity learn more about the function NUBPL, amino acid substitutions were recreated homologous Ind1 protein yeast...
Abstract Plants have evolutionarily conserved NifU (NFU)-domain proteins that are targeted to plastids or mitochondria. “Plastid-type” NFU1, NFU2, and NFU3 in Arabidopsis (Arabidopsis thaliana) play a role iron–sulfur (Fe–S) cluster assembly this organelle, whereas the type-II NFU4 NFU5 not been subjected mutant studies any plant species determine their biological role. Here, we confirmed The were constitutively produced all parts of plant, suggesting housekeeping function. Double nfu4 nfu5...
ABSTRACT During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double nramp3 nramp4 mutant unable to mobilize Fe stores does not develop absence of external Fe. We used RNA sequencing compare gene expression wild type during germination early seedling development. Even though sufficient was supplied, Fe-responsive transcription factors bHLH38, 39, 100 101 their downstream targets FRO2 IRT1 mediating uptake were strongly...
Abstract Complex I deficiency is a common cause of mitochondrial disease, resulting from mutations in genes encoding structural subunits, assembly factors or defects gene expression. Advances genetic diagnostics and sequencing have led to identification several variants NUBPL, an factor complex I, which are potentially pathogenic. To help assign pathogenicity learn more about the function amino acid substitutions were recreated homologous Ind1 protein yeast model Yarrowia lipolytica . L102P...
Abstract Iron-sulfur (Fe-S) clusters are ubiquitous cofactors in all life and used a wide array of diverse biological processes, including electron transfer chains several metabolic pathways. Biosynthesis machineries for Fe-S exist plastids, the cytosol mitochondria. A single monothiol glutaredoxin (GRX) has been shown to be involved cluster assembly mitochondria yeast mammals. In plants, role mitochondrial homologue GRXS15 only partially characterized. Arabidopsis grxs15 null mutants not...
Abstract The mitochondrial electron transport chain (mETC) and F 1 o -ATP synthase are of central importance for energy metabolism in eukaryotic cells. Apicomplexa, important pathogens humans causing diseases such as toxoplasmosis malaria, depend on their mETC every known stage complicated life cycles. Here, using a complexome profiling proteomic approach, we have characterised the Toxoplasma complexes synthase. We identified assigned 60 proteins to II, IV , which 16 not been previously....
Abstract Mitochondria play a key role in the biosynthesis of two metal cofactors, iron-sulfur (FeS) clusters and molybdenum cofactor (Moco). The pathways intersect at several points, but scarcity mutants has hindered studies to better understand these links. We screened collection sirtinol-resistant Arabidopsis thaliana for lines with decreased activities cytosolic FeS enzymes Moco enzymes. identified new mutant allele ATM3 , encoding ATP-binding cassette Transporter 3 (systematic name...
Summary Apicomplexan parasites cause diseases such as malaria and toxoplasmosis. The apicomplexan mitochondrion shows striking differences from common model organisms, including in fundamental processes mitochondrial translation. Despite evidence that translation is essential for survival, it largely understudied. Progress has been restricted by the absence of functional assays to detect translation, a lack knowledge proteins involved process inability identify mitoribosomes. Using mRNA...