A5012902587- Plant Micronutrient Interactions and Effects
- Metalloenzymes and iron-sulfur proteins
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Iron Metabolism and Disorders
- Electrocatalysts for Energy Conversion
- Metal-Catalyzed Oxygenation Mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Trace Elements in Health
- ATP Synthase and ATPases Research
- Enzyme Structure and Function
- Plant Parasitism and Resistance
- Agricultural Science and Fertilization
- Plant and animal studies
- Folate and B Vitamins Research
- Redox biology and oxidative stress
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Cell death mechanisms and regulation
University of East Anglia
2014-2025
John Innes Centre
2015-2025
Norwich Research Park
2012-2022
Norwich University
2017
Heidelberg University
2014
University of Cambridge
2005-2013
University of California, San Diego
2009
Philipps University of Marburg
2003-2007
Joint Genome Institute
2007
Institut de Biologie Physico-Chimique
2007
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It model system for studying chloroplast-based photosynthesis, as well the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited common ancestor animals, but lost in plants. We sequenced ∼120-megabase nuclear genome performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated...
Increasing the intrinsic nutritional quality of crops, known as biofortification, is viewed a sustainable approach to alleviate micronutrient deficiencies. In particular, iron deficiency anemia major global health issue, but content staple crops such wheat (Triticum aestivum) difficult change because genetic complexity and homeostasis mechanisms. To identify target genes for biofortification wheat, we functionally characterized homologs VACUOLAR IRON TRANSPORTER (VIT). The genome contains...
Wheat, like many other staple cereals, contains low levels of the essential micronutrients iron and zinc. Up to two billion people worldwide suffer from zinc deficiencies, particularly in regions with predominantly cereal-based diets. Although wheat flour is commonly fortified during processing, an attractive more sustainable solution biofortification, which requires developing new varieties inherently higher content their grains. Until now most studies aimed at increasing grains have...
In mammals, mitochondria have been shown to play a key intermediary role in apoptosis, morphologically distinct form of programmed cell death (PCD), for example, through the release cytochrome c, which activates proteolytic enzyme cascade, resulting specific nuclear DNA degradation and death. plants, PCD is feature normal development, including penultimate stage anther leading dehiscence pollen release. However, there little evidence that plant are involved PCD. wide range species, and/or...
In mammals mitochondria play a critical role in the activation of programmed cell death (PCD). One mechanism by which can commit to is translocating cytochrome c into cytosol where it activates caspases. However, release does not appear be feature caspase nematodes or insects, similarly, there no evidence for during caspase‐independent PCD that occur Dictyostelium cells. an attempt understand underlying regulation plants we investigated if mitochondrial components were released when plant...
Two transcriptional activators, Aft1 and Aft2, regulate iron homeostasis in Saccharomyces cerevisiae. These factors induce the expression of regulon genes iron-deficient yeast but are inactivated iron-replete cells. Iron inhibition Aft1/Aft2 is abrogated cells defective for Fe-S cluster biogenesis within mitochondrial matrix (Chen, O. S., Crisp, R. J., Valachovic, M., Bard, Winge, D. R., Kaplan, J. (2004) Biol. Chem. 279, 29513-29518). To determine whether sensing by requires function export...
Respiratory complex I (NADH:ubiquinone oxidoreductase) is a large mitochondrial inner membrane enzyme consisting of 45 subunits and 8 iron-sulfur (Fe/S) clusters. While dysfunction the most common reason for diseases, assembly its Fe/S cofactors remains elusive. Here, we identify human P-loop NTPase, designated huInd1, that critically required I. huInd1 can bind an cluster via conserved CXXC motif in labile fashion. Knockdown HeLa cells by RNA interference technology led to strong decreases...
An ATP-binding cassette transporter located in the inner mitochondrial membrane is involved iron-sulfur cluster and molybdenum cofactor assembly cytosol, but transported substrate unknown. ATM3 (ABCB25) from Arabidopsis thaliana its functional orthologue Atm1 Saccharomyces cerevisiae were expressed Lactococcus lactis studied inside-out vesicles purified form. Both proteins selectively glutathione disulfide (GSSG) not reduced agreement with a 3-fold stimulation of ATPase activity by GSSG. By...
Abstract The molybdenum cofactor (Moco) is a prosthetic group required by number of enzymes, such as nitrate reductase, sulfite oxidase, xanthine dehydrogenase, and aldehyde oxidase. Its biosynthesis in eukaryotes can be divided into four steps, which the last three are proposed to occur cytosol. Here, we report that mitochondrial ABC transporter ATM3, previously implicated maturation extramitochondrial iron-sulfur proteins, has crucial role also Moco biosynthesis. In ATM3 insertion mutants...
Organisms need to balance sufficient uptake of iron (Fe) with possible toxicity. In plant roots, a regulon genes is transcriptionally activated under Fe deficiency, but it unknown how this response inactivated when becomes available. Here we describe the function 2 partially redundant E3 ubiquitin ligases, BRUTUS-LIKE1 (BTSL1) and BTSL2, in Arabidopsis thaliana provide evidence that they target transcription factor FIT, key regulator uptake, for degradation. The btsl double mutant failed...
Abstract Wheat is the staple food crop in temperate countries and increasingly consumed developing countries, displacing traditional foods. However, wheat products are typically low bioavailable iron zinc, contributing to deficiencies these micronutrients where as a food. Two factors contribute contents of zinc wheat: concentrations minerals white flour, which most widely consumed, presence phytates mineral‐rich bran fractions. Although high types have been developed by conventional plant...
Soluble P loop NTPases represent a large protein family and are involved in diverse cellular functions. Here, we functionally characterized the first member of Mrp/Nbp35 subbranch this family, essential Nbp35p Saccharomyces cerevisiae . The resides cytosol nucleus carries an Fe/S cluster at its N terminus. Assembly requires mitochondrial (ISC)-assembly -export machineries. Depletion strongly impairs activity cytosolic protein, isopropylmalate isomerase (Leu1p), whereas enzymes unaffected....
Abstract The ATP-binding cassette transporters of mitochondria (ATMs) are highly conserved proteins, but their function in plants is poorly defined. Arabidopsis (Arabidopsis thaliana) has three ATM genes, namely ATM1, ATM2, and ATM3. Using a collection insertional mutants, we show that only ATM3 an important for plant growth. Additional atm3 alleles were identified among sirtinol-resistant lines, correlating with decreased activities aldehyde oxidases, cytosolic enzymes convert sirtinol into...
Previous studies have indicated that the essential protein Nfs1 performs a crucial role in cellular iron-sulfur (Fe/S) maturation. The is located predominantly mitochondria, yet low amounts are present cytosol and nucleus. Here we examined several aspects concerning molecular function of yeast Nfs1p as model protein. First, demonstrated purified facilitates vitro assembly Fe/S proteins by using cysteine its specific substrate. Thus, eukaryotic functional orthologue bacterial desulfurase...
The assembly of cytosolic and nuclear iron-sulfur (Fe/S) proteins in yeast is dependent on the cluster export machineries mitochondria three recently identified extramitochondrial proteins, P-loop NTPases Cfd1 Nbp35 hydrogenase-like Nar1.However, molecular mechanism Fe/S protein cytosol far from being understood, more components are anticipated to take part this process.Here, we have functionally characterized a novel WD40 repeat protein, designated Cia1, as an essential component required...