A5031509890- Epigenetics and DNA Methylation
- Metalloenzymes and iron-sulfur proteins
- Pancreatitis Pathology and Treatment
- RNA modifications and cancer
- RNA Research and Splicing
- Galectins and Cancer Biology
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- Cellular transport and secretion
- Pancreatic function and diabetes
- Cancer-related Molecular Pathways
- Hereditary Neurological Disorders
- Genomics and Chromatin Dynamics
- Peptidase Inhibition and Analysis
- Calcium signaling and nucleotide metabolism
- Endoplasmic Reticulum Stress and Disease
- Skin and Cellular Biology Research
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Advanced biosensing and bioanalysis techniques
- Cancer and Skin Lesions
- Cancer Mechanisms and Therapy
Loewe Center for Synthetic Microbiology
2024
Philipps University of Marburg
2009-2024
Springer Nature (Germany)
2021
University of Ottawa
2012
University of Göttingen
2000
Southwestern Medical Center
1995-1999
The University of Texas Southwestern Medical Center
1995-1999
King's College London
1995
Zentrum für Pathologie und Zytodiagnostik
1989
Heinrich Heine University Düsseldorf
1984
Mammalian adrenodoxin (ferredoxin 1; Fdx1) is essential for the synthesis of various steroid hormones in adrenal glands. As a member [2Fe-2S] cluster-containing ferredoxin family, Fdx1 reduces mitochondrial cytochrome P450 enzymes, which then catalyze; e.g., conversion cholesterol to pregnenolone, aldosterone, and cortisol. The high protein sequence similarity between its yeast homologue (Yah1) suggested that Fdx1, like Yah1, may be involved biosynthesis heme A Fe/S clusters, two versatile...
Members of the bacterial and mitochondrial iron-sulfur cluster (ISC) assembly machinery include so-called A-type ISC proteins, which support a subset Fe/S apoproteins. The human genome encodes two termed ISCA1 ISCA2, are related to Saccharomyces cerevisiae Isa1 Isa2, respectively. An additional protein, Iba57, physically interacts with Isa2 in yeast. To test cellular role ISCA1, IBA57, HeLa cells were depleted for any these proteins by RNA interference technology. Depleted contained...
We searched for novel proteins in lysosomal membranes, tentatively participating molecular transport across the membrane and/or interactions with other compartments. In membranes purified from placental lysosomes, we identified 58 proteins, known to reside at least partially membrane. These included 17 polypeptides comprising or associated vacuolar adenosine triphosphatase. report on additional 86 that were significantly enriched fraction. Among these, 12 of unknown functions found. Three...
Respiratory complex I (NADH:ubiquinone oxidoreductase) is a large mitochondrial inner membrane enzyme consisting of 45 subunits and 8 iron-sulfur (Fe/S) clusters. While dysfunction the most common reason for diseases, assembly its Fe/S cofactors remains elusive. Here, we identify human P-loop NTPase, designated huInd1, that critically required I. huInd1 can bind an cluster via conserved CXXC motif in labile fashion. Knockdown HeLa cells by RNA interference technology led to strong decreases...
Polyelectrolyte multilayer (PEM) capsules are carrier vehicles with great potential for biomedical applications. With the future aim of designing biocompatible, effective therapeutic delivery systems (e.g., cancer), pathway internalization (uptake and fate) PEM was investigated. In particular following experiments were performed: (i) study capsule co-localization established endocytic markers, (ii) switching-off endocytotic pathways pharmaceutical/chemical inhibitors, (iii) characterization...
The biogenesis of iron-sulfur (Fe/S) proteins in eukaryotes is a complex process involving more than 20 components. So far, functional investigations have mainly been performed Saccharomyces cerevisiae. Here, we analyzed the role human cysteine desulfurase Nfs1 (huNfs1), which serves as sulfur donor biogenesis. protein located predominantly mitochondria, but small amounts are present cytosol/nucleus. huNfs1 was depleted efficiently HeLa cells by interfering RNA (siRNA) approach, resulting...
Significance Lacking a canonical signal peptide for translocation into the endoplasmic reticulum, galectin-3 (Gal3) is exported by unconventional secretion to play key roles in various cellular processes of fundamental importance. However, molecular details about sorting components, signals involved, and underlying mechanism have remained elusive. Here, we identify highly conserved tetrapeptide motif P(S/T)AP amino-terminal domain Gal3 that directly interacts with endosomal complex required...
The maturation of iron-sulfur (Fe/S) proteins in eukaryotes has been intensively studied yeast. Hardly anything is known so far about the process higher eukaryotes, even though high conservation yeast components most Eukarya suggests similar mechanisms. Here, we developed a cell culture model which RNA interference (RNAi) technology was used to deplete potential component Fe/S protein maturation, frataxin, human HeLa cells. This lowered humans with neuromuscular disorder Friedreich's ataxia...
The regenerative capacity of the different cell types in rat exocrine pancreas has been studied a model hormone-induced acute pancreatitis which pancreatic edema, inflammation, and acinar destruction were induced within 12 h infusion supramaximal concentrations cerulein (5 μg/kg/h). A sequential biochemical structural analysis daily intervals was combined with autoradiographic quantitation labeling indices five populations following 3H-thymidine injection at days 1–7 after induction...
Myc plays a key role in homeostasis of the skin. We show that Miz1, which mediates repression gene expression, is expressed epidermal basal layer. A large percentage genes regulated by Myc-Miz1 complex keratinocytes encode proteins involved cell adhesion, and some, including alpha6 beta1 integrins, are directly bound Miz1 vivo. Using mutant deficient binding (MycV394D), we required for effects on keratinocyte responsiveness to TGF-beta. Myc, but not MycV394D, decreases adhesion spreading. In...
The autofluorescent substance monodansylcadaverine has recently been reported as a specific in vivo marker for autophagic vacuoles. However, the mechanism this labeling remained unclear. Our results reveal that common model of ion trapping acidic compartments cannot completely account observed vacuole staining. Because vacuoles are characterized by myelin-like membrane inclusions, we tested whether lipid-rich environment is responsible staining properties monodansylcadaverine. In vitro...
Four molecules of the tumor suppressor p53 assemble to cooperatively bind proapoptotic target genes. The structural basis for cooperativity consists interactions between adjacent DNA binding domains. Mutations at interaction interface that compromise were identified in cancer patients, suggesting a requirement suppression. We report on an analysis mutant p53E177R mice. Apoptotic functions triggered by damage and oncogenes abolished these mice, whereas cell-cycle control, senescence,...
Eighty percent of human ovarian and endometrial cancers express receptors for luteinizing hormone-releasing hormone (LHRH). These might be used targeted chemotherapy with cytotoxic LHRH analogs such as AN-152, in which doxorubicin is linked to agonist carrier [D-Lys6]LHRH. The antiproliferative effects AN-152 were assessed receptor-positive (EFO-21, EFO-27) (HEC-1A, Ishikawa) cancer cell lines well receptor negative SKOV-3 MFE-296 lines. mechanism action was investigated by a blockage using...
Polyethylenimines (PEIs) of a molecular weight between 25 and about 800 kDa have successfully been used for in vitro vivo gene delivery approaches. Recent publications indicated that PEI molecules lower small range are also efficient transfection reagents with much cytotoxicity compared to high PEIs. Here, we describe the application sieve chromatography fractionate commercially available 25-kDa PEI. We generated three pools PEIs ranges 70-360 (I), 10-70 (II), 0.5-10 (III), respectively....