A5017859454- Advanced biosensing and bioanalysis techniques
- Polydiacetylene-based materials and applications
- RNA Interference and Gene Delivery
- Supramolecular Self-Assembly in Materials
- Electrochemical sensors and biosensors
- DNA and Nucleic Acid Chemistry
- Carbon Nanotubes in Composites
- Dendrimers and Hyperbranched Polymers
- Luminescence and Fluorescent Materials
- Advanced Biosensing Techniques and Applications
- Polymer Surface Interaction Studies
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Photoacoustic and Ultrasonic Imaging
- Molecular Sensors and Ion Detection
- Nanoparticles: synthesis and applications
- Photosynthetic Processes and Mechanisms
- Blood properties and coagulation
- Graphene and Nanomaterials Applications
- Conducting polymers and applications
- RNA and protein synthesis mechanisms
- Molecular Junctions and Nanostructures
- Analytical Chemistry and Sensors
- Nanoparticle-Based Drug Delivery
Tel Aviv University
2020-2025
Hebrew University of Jerusalem
2018-2019
Center for NanoScience
2018
Johannes Gutenberg University Mainz
2016
Philipps University of Marburg
2012-2013
Goethe University Frankfurt
2012
Nanomaterials offer opportunities to construct novel compounds for many different fields. Applications include devices energy, including solar cells, batteries, and fuel health, contrast agents mediators photodynamic therapy hyperthermia. Despite these promising applications, any new class of materials also bears a potential risk human health the environment. The advantages innovations must be thoroughly compared against risks evaluate each nanomaterial. Although nanomaterials are often used...
Colloidal nanoparticles (NPs) have become versatile building blocks in a wide variety of fields. Here, we discuss the state-of-the-art, current hot topics, and future directions based on following aspects: narrow size-distribution NPs can exhibit protein-like properties; monodispersity is not always required; assembled collective behavior; be one by one; there more to connected with NPs; designed smart; surface-modified directly reach cytosols living cells.
Polyelectrolyte multilayer (PEM) capsules are carrier vehicles with great potential for biomedical applications. With the future aim of designing biocompatible, effective therapeutic delivery systems (e.g., cancer), pathway internalization (uptake and fate) PEM was investigated. In particular following experiments were performed: (i) study capsule co-localization established endocytic markers, (ii) switching-off endocytotic pathways pharmaceutical/chemical inhibitors, (iii) characterization...
Gold nanoparticles (AuNPs) or gold nanorods (AuNRs) are loaded in polyacrylamide hydrogels cooperatively cross-linked by bis-acrylamide and nucleic acid duplexes boronate ester–glucosamine duplexes. The thermoplasmonic properties of AuNPs AuNRs used to control the stiffness hydrogels. irradiation AuNP-loaded (λ = 532 nm) AuNR-loaded 808 leads heating hydrogels, dehybridization DNA duplexes, formation with lower stiffness. By ON/OFF irradiation, switched between low- high-stiffness states....
Stimuli-responsive polyacrylamide hydrogels crosslinked by glucosamine–boronate/G-quadruplexes or azobenzene-functionalized DNA reveal controlled stiffness using chemical photochemical triggers.
Abstract Constitutional dynamic networks (CDNs) attract interest as signal-triggered reconfigurable systems mimicking natural networks. The application of CDNs to control material properties is, however, a major challenge. Here we report on the design CDN consisting four toehold-modified constituents, two which act bidentate units for chain-elongating, while other form tetradentate structure acting crosslinking unit. Their hybridization yields hydrogel medium stiffness controlled by balance...
MicroRNAs (miRNAs) are single-stranded non-coding short ribonucleic acid sequences that take part in many cellular and biological processes. Recent studies have shown altered expression of miRNAs is involved pathological processes, they can thus be considered biomarkers for the early detection various diseases. Here, we demonstrate a selection elimination process fluorescent single-walled carbon nanotube (SWCNT) sensors miRNA based on RNA-DNA hybridization with complementary DNA recognition...
Blood coagulation is a critical defense mechanism against bleeding that results in the conversion of liquid blood into solid clot through complicated cascade, which involves multiple clotting factors. One final steps pathway fibrinogen to insoluble fibrin mediated by thrombin. Because disorders can be life-threatening, development novel methods for monitoring cascade dynamics high importance. Here, we use near-infrared (NIR)-fluorescent single-walled carbon nanotubes (SWCNTs) image and...
Accurate analyte classification remains a significant challenge in sensor technologies. We present the Analyte Classification and Feature Selection Algorithm (ACFSA), computational tool designed to identify optimal combinations from unique fingerprint patterns for classification. applied ACFSA library of peptide-corona-functionalized single-walled carbon nanotubes (SWCNTs), developed as near-infrared fluorescent, semiselective fingerprinting set detecting heavy metal ions. Inspired by...
Intercommunication between dynamic chemical networks plays a major role in cellular transformations. Inspired by nature, we introduce the intercommunication two constitutional networks, CDNs, "S" and "T" composed, each, of four equilibrated supramolecular constituents AA′, AB′, BA′, BB′, CC′, CD′, DC′, DD′, respectively. Each is conjugated to Mg2+-ion-dependent DNAzyme unit that acts as reporter element for concentration respective constituent via catalyzed cleavage...
In this study, we report on a redox-controllable and reversible complete "ON"/"OFF"-switchable aptamer binding to ATP. A series of methylene blue-modified ATP–aptamers was synthesized, revealing improved affinities toward ATP as compared the nonmodified aptamer. These were dependent conjugation site redox label scaffold. Importantly, find that oxidized aptamers bind with micromolar affinity, while reduced form lacks affinity ATP, resulting in an unprecedented "ON"/"OFF" switch. We...
Abstract Quantification of insulin is essential for diabetes research in general, and the study pancreatic β‐cell function particular. Herein, fluorescent single‐walled carbon nanotubes (SWCNT) are used recognition real‐time quantification insulin. Two approaches rendering SWCNT sensors compared, using surface functionalization with either a natural aptamer known affinity to insulin, or synthetic lipid‐poly(ethylene glycol) (PEG) (C 16 ‐PEG(2000Da)‐Ceramide), both which show modulation...
Abstract The accumulation of metal ions in organisms and the presence heavy metals water cause adverse effects on ecosystems results numerous human health issues such as cancer neurogenerative diseases. Therefore, development novel platforms for metal‐scavenging rapid detection situ applications are high importance. Here, this challenge is tackled by taking advantage chelation ability a melanin‐inspired material combination with near‐infrared (NIR) fluorescence response single‐walled carbon...
Hydrogels formed via supramolecular self-assembly of fluorenylmethyloxycarbonyl (Fmoc)-conjugated amino acids provide excellent scaffolds for 3D cell culture, tissue engineering, and recovery matrices. Such hydrogels are usually characterized by rheology or electron microscopy, which invasive cannot real-time information. Here, we incorporate near-infrared fluorescent single-walled carbon nanotubes (SWCNTs) into Fmoc-diphenylalanine as probes, reporting in on the morphology time-dependent...
Cellular transformations are driven by environmentally triggered complex dynamic networks, which include signal-triggered feedback processes, cascaded reactions, and switchable transformations. We apply the structural functional information encoded in sequences of nucleic acids to construct constitutional networks (CDNs) that mimic functions natural networks. Using predesigned hairpin structures as triggers, network generates strands, stabilize one or other constituents network, leading...
Following the significance of dissipative, out-of-equilibrium biological processes controlling living systems, we introduce nucleic acid-based dissipative constitutional dynamic networks (CDNs) that exhibit tunable transient composition changes dictated by auxiliary fuel strands. CDN "X" composed four equilibrated acid constituents, AA′, AB′, BA′, and BB′, accompanying "dormant" structures T1L1 T2L2 nicking enzyme Nt.BbvCI, undergoes orthogonal transitions to "Y" back or "Z" back. In...
Caenorhabditis elegans (C. elegans) nematodes serve as a model organism for eukaryotes, especially due to their genetic similarity. Although they have many advantages like small size and transparency, autofluorescence in the entire visible wavelength range poses challenge imaging tracking fluorescent proteins or dyes using standard fluorescence microscopy. Herein, near-infrared (NIR) single-walled carbon nanotubes (SWCNTs) are utilized vivo within gastrointestinal track of C. elegans. The...
Abstract The production of oncometabolites is the direct result mutagenesis in key cellular metabolic enzymes, appearing typically cancers such as glioma, leukemia, and glioblastoma. Once accumulated, promote cancerous transformations by interfering with important functions. Hence, ability to sense quantify essential for cancer research clinical diagnosis. Here, authors present a near‐infrared optical nanosensor known oncometabolite, D‐2‐hydroxyglutarate (D2HG), discovered screening library...
Functional composite materials that can change their spectral properties in response to external stimuli have a plethora of applications fields ranging from sensors biomedical imaging. One the most promising types used design spectrally active composites are fluorescent single-walled carbon nanotubes (SWCNTs), noncovalently functionalized by synthetic amphiphilic polymers. These coated SWCNTs exhibit modulations fluorescence spectra interactions with target analytes. Hence, identifying new...
Abstract The evolution of networks is a fundamental unresolved issue in developing the area systems chemistry. We introduce versatile rewiring mechanism that leads to emergence nucleic‐acid‐based constitutional dynamic (CDNs). A two‐component constituent AA′ functionalized with Mg 2+ ‐ion‐dependent DNAzyme activator unit forms complex an intact hairpin H BB′ composed B and B′ sequences. Cleavage BB′, its yields CDN X equilibrated constituents AA′, AB′, BA′, BB′. In analogy, subjecting CC′...
Plasmonic gold nanoparticles are normally used as sensor to detect analytes permanently bound their surface. If the interaction between analyte and nanosensor surface is negligible, it only diffuses through sensor's sensing volume, causing a small temporal shift of plasmon resonance position. By using very sensitive fast detection scheme, we able these fluctuations in resonance. With help theoretical model consistent with our geometry, determine analyte's diffusion coefficient. The method...
Single‐walled carbon nanotubes (SWCNTs) have unique optical and physical properties, with numerous biomedical imaging sensing applications, owing to their near‐infrared (nIR) fluorescence which overlaps the biological transparency window. However, longer emission wavelengths compared emitters in visible range result a lower resolution due diffraction limit. Moreover, elongated high‐aspect‐ratio structure of SWCNTs poses an additional challenge on super‐resolution techniques that assume point...