The generally low bioavailability of iron in aerobic soil systems forced plants to evolve sophisticated genetic strategies improve the acquisition from sparingly soluble and immobile pools. To distinguish between conserved species-dependent components such strategies, we analyzed deficiency-induced changes transcriptome two model species, Arabidopsis (Arabidopsis thaliana) Medicago truncatula. Transcriptional profiling by RNA sequencing revealed a massive up-regulation genes coding for...

Due to its ease donate or accept electrons, iron (Fe) plays a crucial role in respiration and metabolism, including tetrapyrrole synthesis, virtually all organisms. In plants, Fe is component of the photosystems thus essential for photosynthesis. deficiency compromises chlorophyll (Chl) leading interveinal chlorosis developing leaves decreased photosynthetic activity. To gain insights into responses photosynthetically active cells deficiency, we conducted transcriptional profiling...

Iron (Fe) is an essential mineral nutrient and important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to evolution elaborate mechanisms that extract from soil solution. In Arabidopsis (Arabidopsis thaliana), acquired by orchestrated strategy comprises mobilization, chelation, reduction Fe3+ prior its uptake. Here, we show At3g12900, previously annotated as scopoletin 8-hydroxylase (S8H), participates...

Organisms need to balance sufficient uptake of iron (Fe) with possible toxicity. In plant roots, a regulon genes is transcriptionally activated under Fe deficiency, but it unknown how this response inactivated when becomes available. Here we describe the function 2 partially redundant E3 ubiquitin ligases, BRUTUS-LIKE1 (BTSL1) and BTSL2, in Arabidopsis thaliana provide evidence that they target transcription factor FIT, key regulator uptake, for degradation. The btsl double mutant failed...

Plants grown under iron deficiency show different morphological, biochemical and physiological changes. These changes include, among others, the elicitation of strategies to improve acquisition Fe from rhizosphere, adjustment homeostasis processes a reorganization carbohydrate metabolism. The application modern techniques that allow simultaneous untargeted analysis multiple proteins metabolites can provide insight into taking place in plants deficiency. objective this study was characterize...

Iron deficiency is a yield-limiting factor with major implications for field crop production in one-third of the world's agricultural areas, especially those high soil CaCO3. In present work, two-dimensional gel electrophoresis proteomic approach was combined study on riboflavin synthesis pathway, including qPCR and determination, to investigate Fe-deficiency responses Medicago truncatula plants grown without caused de novo accumulation DMRLs GTPcII, proteins involved biosynthesis, as well...

The root accumulation and excretion of riboflavin (Rbfl) Rbfl derivatives have been studied in the model legume species Medicago truncatula, grown hydroponics two different Fe deficiency conditions, with without CaCO3. Using high resolution mass spectrometry techniques coupled to liquid chromatography, three flavin not previously reported plants, putatively identified as 7-hydroxy-Rbfl, 7α-hydroxy-Rbfl 7-carboxy-Rbfl, were found along Fe-deficient M. truncatula roots. In presence CaCO3 most...

Pea seeds are widely consumed in their immature form, known as garden peas and petit pois, mostly after preservation by freezing or canning. Mature dry rich iron the form of ferritin, but little is about content, bioavailability stages seed development. Using specific antibodies in-gel staining, we show that ferritin loaded with accumulated gradually during Immunolocalization high-resolution secondary ion mass spectrometry (NanoSIMS) revealed iron-loaded was located at surface...

Iron deficiency is an enduring global health problem that requires new remedial approaches. absorption from soybean-derived ferritin, ∼550-kDa iron storage protein, comparable to bioavailable ferrous sulfate (FeSO4). However, the of ferritin reported involve endocytic mechanism, independent divalent metal ion transporter 1 (DMT-1), for nonheme iron. Our overall aim was examine potential purified peas (Pisum sativum) as a food supplement by measuring its stability under gastric pH treatment...

The mineral micronutrients zinc (Zn) and iron (Fe) are essential for plant growth human nutrition, but interactions between the homeostatic networks of these two elements not fully understood. Here we show that loss function BTSL1 BTSL2, which encode partially redundant E3 ubiquitin ligases negatively regulate Fe uptake, confers tolerance to Zn excess in Arabidopsis thaliana. Double btsl1 btsl2 mutant seedlings grown on high medium accumulated similar amounts roots shoots wild type,...

With the exception of grasses, plants rely on a reduction-based iron (Fe) uptake system that is compromised by high soil pH, leading to severe chlorosis and reduced yield in crop plants. We recently reported deficiency triggers production secondary metabolites are beneficial for Fe particular at external pH when present but not readily available. The exact function these metabolites, however, remains enigmatic. Here, we speculate mechanism which secreted roots from Fe-deficient improve...

Protein profiles of inner (IE) and outer (OE) chloroplast envelope membrane preparations from pea were studied using shotgun nLC-MS/MS two-dimensional electrophoresis, 589 protein species (NCBI entries) identified. The relative enrichment each in the IE/OE pair membranes was used to provide an integrated picture envelope. From 546 proteins identified with shotgun, 321 showed a significant differential distribution, 180 being enriched IE 141 OE. To avoid redundancy facilitate silico...

The changes in the root extract protein profile of Prunus hybrid GF 677 rootstock (P. dulcis × P. persica) grown hydroponics as affected by Fe deficiency and short-term (24 h) resupply have been studied 2-dimensional gel electrophoresis-based techniques. A total 335 spots were consistently found gels. Iron caused above 2-fold increases or >50% decreases relative abundance 10 6 spots, respectively, whereas one spot was only detected Fe-deficient plants. to plants 15 16 more Fe-resupplied...

Abstract Manganese (Mn) is pivotal for plant growth and development, but little information available regarding the strategies that evolved to improve Mn acquisition cellular homeostasis of Mn. Using an integrated RNA-based transcriptomic high-throughput shotgun proteomics approach, we generated a comprehensive inventory transcripts proteins showed altered abundance in response deficiency roots model Arabidopsis . A suite 22,385 was consistently detected three RNA-seq runs; LC-MS/MS-based...

Iron (Fe) and manganese (Mn) are two essential elements for plants that compete the same uptake transporters show conflicting interactions at regulatory level. In order to understand differential response both metal deficiencies in plants, proteomic techniques (two-dimensional gel electrophoresis label-free shotgun) were used study proteome profiles of roots from tomato grown under Fe or Mn deficiency. A total 119 proteins changing relative abundance confidently quantified identified,...

ABSTRACT Iron is an essential micronutrient but in excess toxic inside cells. Under iron deficiency, the expression of uptake genes increased, it not known how transcriptional response controlled to avoid too much iron. The hemerythrin E3 ligases BRUTUS (BTS) and BTS-LIKE (BTSL) have previously been identified as negative regulators deficiency response. Our phylogenetic analysis indicated that BTSL proteins are present dicotyledonous plants only form a separate clade from BTS homologs. BTSL1...